Easy gene walking

BioTechniques

Volumn 22, Issue 4, 1997, Pages 650-653

  Harrison, Robert W ^a,b   Miller, Janice C ^a   D'Souza, Mary J ^a   Kampo, George ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE;

ANIMAL CELL; ARTICLE; DNA DEGRADATION; DNA SEQUENCE; GENE AMPLIFICATION; GENETIC TRANSFECTION; GOOD LABORATORY PRACTICE; HYPOPHYSIS CELL; MOUSE; NONHUMAN;

EID: 0030972395     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224bm17     Document Type: Article

References (13)

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2. Devon, R.S., D.J. Porteous and A.J. Brookes. 1995. Splinkerettes - improved veclorettes for greater efficiency in PCR walking. Nucleic Acids Res. 23:1644-1645.
3. Dominguez, O. and C. Lopez-Larrea. 1994. Gene walking by unpredictably primed PCR. Nucleic Acids Res. 22:3247-3248.
4. Fors, L., R.A. Saavedra and L. Hood. 1990. Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction. Nucleic Acids Res. 18:2793-2799.
5. Ghiso, N.S., H. Parekh and G.G. Lennon. 1993. A subset of 1200 hexamers is sufficient to sequence over 95% of cDNAs by hexamer string primer walking. Genomics 17:798-799.
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9. Jones, D.H. and S.C. Winistorfer. 1993. Genome walking with 2-4-kb steps using pan-handle PCR. PCR Methods Appl. 2:197-203.
10. Rosenthal, A. and D.S. Jones. 1990. Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction. Nucleic Acids Res. 18:3095-3096.
11. Sarkar, G.S., R.T. Turner and M.E. Bolander. 1993. Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers. PCR Methods Appl. 2:318-322.
12. Screaton, G.R., C.R. Bangham and J.I. Bell. 1993. Direct sequencing of single primer PCR products: a rapid method to achieve short chromosomal walks. Nucleic Acids Res. 21:2263-2264.
13. Siebert, P.D., A. Chenchik, D.E. Kellogg, K.A. Lukyanov and S.A. Lukyanov. 1995. An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res. 23:1087-1088.