Generating transgenic mice from bacterial artificial chromosomes: Transgenesis efficiency, integration and expression outcomes

Transgenic Research

Volumn 18, Issue 5, 2009, Pages 769-785

  van Keuren, Margaret L ^a   Gavrilina, Galina B ^a   Filipiak, Wanda E ^a   Zeidler, Michael G ^a   Saunders, Thomas L ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

BAC; Chromosomes, artificial, bacterial; DNA; Electrophoresis, gel, pulsed field; Gene expression; Gene transfer techniques; Mice, transgenic

Indexed keywords

BACTERIAL DNA;

ANIMAL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BIOSYNTHESIS; GENE TRANSFER; GENETICS; MOUSE; TRANSGENIC MOUSE;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DNA, BACTERIAL; GENE TRANSFER TECHNIQUES; MICE; MICE, TRANSGENIC;

ANIMALIA; BACTERIA (MICROORGANISMS); MUS; MUS MUSCULUS;

EID: 70349977406     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-009-9271-2     Document Type: Article

References (46)

1. Antoch MP, Song EJ, Chang AM, Vitaterna MH, Zhao Y, Wilsbacher LD, Sangoram AM, King DP, Pinto LH, Takahashi JS (1997) Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. Cell 89:655-667. doi:10.1016/S0092-8674(00)80246-9.
2. Baoan J, Song J, Tsou L, Bi Y, Gaiser S, Mortensen R, Logsdon C (2008) Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis 35:390-395.
3. Bishop JO (1996) Chromosomal insertion of foreign DNA. Reprod Nutr Dev 36:607-618.
4. Brinster RL, Chen HY, Trumbauer ME, Yagle MK, Palmiter RD (1985) Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci USA 82:4438-4442. doi:10.1073/pnas.82.13.4438.
5. Bronson SK, Plaehn EG, Kluckman KD, Hagaman JR, Maeda N, Smithies O (1996) Single-copy transgenic mice with chosen-site integration. Proc Natl Acad Sci USA 93:9067-9072. doi:10.1073/pnas.93.17.9067.
6. Callow MJ, Stoltzfus LJ, Lawn RM, Rubin EM (1994) Expression of human apolipoprotein B and assembly of lipoprotein(a) in transgenic mice. Proc Natl Acad Sci USA 91:2130-2134. doi:10.1073/pnas.91.6.2130.
7. Camper SA, Saunders TL (2000) Transgenic rescue of mutant phenotypes using large DNA fragments. In: Accili D (ed) Genetic manipulation of receptor expression and function. Wiley-Liss, New York, pp 1-22.
8. Chandler KJ, Chandler RL, Broeckelmann EM, Hou Y, Southard-Smith EM, Mortlock DP (2007) Relevance of BAC transgene copy number in mice: transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression. Mamm Genome 18:693-708. doi:10.1007/s00335-007-9056-y.
9. Chiesa G, Johnson DF, Yao Z, Innerarity TL, Mahley RW, Young SG, Hammer RH, Hobbs HH (1993) Expression of human apolipoprotein B100 in transgenic mice. Editing of human apolipoprotein B100 mRNA. J Biol Chem 268:23747-23750.
10. Cole KD, Tellez CM (2002) Separation of large circular DNA by electrophoresis in agarose gels. Biotechnol Prog 18:82-87. doi:10.1021/bp010135o.
11. Court DL, Sawitzke JA, Thomason LC (2002) Genetic engineering using homologous recombination. Annu Rev Genet 36:361-388. doi:10.1146/annurev.genet.36.061102.093104.
12. Deal KK, Cantrell VA, Chandler RL, Saunders TL, Mortlock DP, Southard-Smith EM (2006) Distant regulatory elements in a Sox10-betaGEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues. Dev Dyn 235:1413-1432. doi:10.1002/dvdy.20769.
13. Dunnick WA, Shi J, Graves KA, Collins JT (2004) Germline transcription and switch recombination of a transgene containing the entire H chain constant region locus: effect of a mutation in a STAT6 binding site in the gamma1 promoter. J Immun 173:5531-5539.
14. Dunnick WA, Shi J, Graves KA, Collins JT (2005) The 3′ end of the heavy chain constant region locus enhances germline transcription and switch recombination of the four gamma genes. J Exp Med 201:1459-1466. doi:10.1084/jem.20041988.
15. Fiorenza MT, Bevilacqua A, Bevilacqua S, Mangia F (2001) Growing dictyate oocytes, but not early preimplantation embryos, of the mouse display high levels of DNA homologous recombination by single-strand annealing and lack DNA nonhomologous end joining. Dev Biol 233:214-224. doi:10.1006/dbio.2001.0199.
16. Giraldo P, Montoliu L (2001) Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res 10:83-103. doi:10.1023/A:1008918913249.
17. Hammer RE, Krumlauf R, Camper SA, Brinster RL, Tilghman SM (1987) Diversity of alpha-fetoprotein gene expression in mice is generated by a combination of separate enhancer elements. Science 235:53-58. doi:10.1126/science.2432657.
18. Jones JM, Datta P, Srinivasula SM, Ji W, Gupta S, Zhang Z, Davies E, Hajnocyzky SaundersTL, van Keuren ML, Fernandes-Alnemri T, Meisler MH, Alnemri ES (2003) Loss of Omi mitochondrial protease activity causes the neuromuscular disorder of mnd2 mutant mice. Nature 425:721-727. doi:10.1038/nature02052.
19. Keegan CE, Karolyi IJ, Burrows H, Camper SA, Seasholtz AF (1994) Homologous recombination in fertilized mouse eggs and assessment of heterologous LCR function. Transgenics 1:439-449.
20. Kjer-Nielsen L, Holmberg K, Perera JD, McCluskey J (1992) Impaired expression of chimaeric major histocompatibility complex transgenes associated with plasmid sequences. Transgenic Res 1:182-187. doi:10.1007/BF02522537.
21. Krebs CJ, Larkins LK, Price R, Tullis KM, Miller RD, Robins DM (2003) Regulator of sex-limitation (Rsl) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression. Genes Dev 17:2664-2674. doi:10.1101/gad.1135703.
22. Larin Z, Monaco AP, Lehrach H (1991) Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc Natl Acad Sci USA 88:4123-4127. doi:10.1073/pnas.88.10.4123.
23. Lehoczky JA, Innis JW (2008) BAC transgenic analysis reveals enhancers sufficient for Hoxa13 and neighborhood gene expression in mouse embryonic distal limbs and genital bud. Evol Dev 10:421-432. doi:10.1111/j.1525-142X.2008.00253.x.
24. Lo CW (1986) Localization of low abundance DNA sequences in tissue sections by in situ hybridization. J Cell Sci 81:143-162.
25. Luciw PA, Bishop JM, Varmus HE, Capecchi MR (1983) Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. Cell 33:705-716.
26. Mensah-Osman E, Labut E, Zavros Y, El-Zaatari M, Law DJ, Merchant JL (2008) Regulated expression of the human gastrin gene in mice. Regul Pept 151:115-122. doi:10.1016/j.regpep.2008.03.009.
27. Montoliu L, Bock CT, Schutz G, Zentgraf H (1995) Visualization of large DNA molecules by electron microscopy with polyamines: application to the analysis of yeast endogenous and artificial chromosomes. J Mol Biol 246:486-492. doi:10.1006/jmbi.1994.0100.
28. Nielsen LB, McCormick SP, Pierotti V, Tam C, Gunn MD, Shizuya H, Young SG (1997) Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes. Evidence that a distant 5′-element confers appropriate transgene expression in the intestine. J Biol Chem 272:29752-29758. doi:10.1074/jbc.272.47.29752.
29. Okita K, Ichisaka T, Yamanaka S (2007) Generation of germline-competent induced pluripotent stem cells. Nature 448:313-317. doi:10.1038/nature05934.
30. Oliver ER, Saunders TL, Tarlé SA, Glaser T (2004) Ribosomal protein L24 defect in Belly spot and tail (Bst), a mouse Minute. Development 131:3907-3920. doi:10.1242/dev.01268.
31. Osoegawa K, Tateno M, Woon PY, Frengen E, Mammoser AG, Catanese JJ, Hayashizaki Y, de Jong PJ (2000) Bacterial artificial chromosome libraries for mouse sequencing and functional analysis. Genome Res 10:116-128.
32. Probst FJ, Fridell RA, Raphael Y, Saunders TL, Wang A, Liang Y, Morell RJ, Touchman JW, Lyons RH, Noben-Trauth K, Friedman TB, Camper SA (1998) Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene. Science 280:1444-1447. doi:10.1126/science.280.5368.1444.
33. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491. doi:10.1126/science.2448875.
34. Schedl A, Larin Z, Montoliu L, Thies E, Kelsey G, Lehrach H, Schütz G (1993a) A method for the generation of YAC transgenic mice by pronuclear microinjection. Nucleic Acids Res 21:4783-4787. doi:10.1093/nar/21.20.4783.
35. Schedl A, Montoliu L, Kelsey G, Schutz G (1993b) A yeast artificial chromosome covering the tyrosinase region confers copy number dependent expression in transgenic mice. Nature 362:258-261. doi:10.1038/362258a0.
36. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503-517. doi:10.1016/S0022-2836(75)80083-0.
37. Sparwasser T, Gong S, Li JY, Eberl G (2004) General method for the modification of different BAC types and the rapid generation of BAC transgenic mice. Genesis 38:39-50. doi:10.1002/gene.10249.
38. Sun H, Yang TL, Yang A, Wang X, Ginsburg D (2003) The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis. Blood 102:2856-2861. doi:10.1182/blood-2003-04-1225.
39. Tacken PJ, van der Zee A, Beumer TL, Florijn RJ, Gijpels MJ, Havekes LM, Frants RR, van Dijk KW, Hofker MH (2001) Effective generation of very low density lipoprotein receptor transgenic mice by overlapping genomic DNA fragments: high testis expression and disturbed spermatogenesis. Transgenic Res 10:211-221. doi:10.1023/A:1016682520887.
40. Testa G, Zhang Y, Vintersten K, Benes V, Pijnappel WW, Chambers I, Smith AJ, Smith AG, Stewart AF (2003) Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles. Nat Biotechnol 21:443-447. doi:10.1038/nbt804.
41. Townes TM, Lingrel JB, Chen HY, Brinster RL, Palmiter RD (1985) Erythroid-specific expression of human beta-globin genes in transgenic mice. EMBO J 4:1715-1723.
42. Wagner SD, Gross G, Cook GP, Davies SL, Neuberger MS (1996) Antibody expression from the core region of the human IgH locus reconstructed in transgenic mice using bacteriophage P1 clones. Genomics 35:405-414. doi:10.1006/geno.1996.0379.
43. Wang M, Lai E (1995) Pulsed field separation of large supercoiled and open-circular DNAs and its application to bacterial artificial chromosome cloning. Electrophoresis 16:1-7. doi:10.1002/elps.1150160102.
44. Wilson C, Bellen HJ, Gehring WJ (1990) Position effects on eukaryotic gene expression. Annu Rev Cell Biol 6:679-714. doi:10.1146/annurev.cb.06.110190.003335.
45. Zar JH (1998) Biostatistical analysis, 4th edn. Prentice Hall, Upper Saddle River.
46. Zeller W, Meier G, Burki K, Panoussis B (1998) Adverse effects of tribromoethanol as used in the production of transgenic mice. Lab Anim 32:407-413. doi:10.1258/002367798780599811.