Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene

Science

Volumn 280, Issue 5368, 1998, Pages 1444-1447

  Probst, Frank J ^a   Fridell, Robert A ^b   Raphael, Yehoash ^e   Saunders, Thomas L ^a   Wang, Aihui ^c,d   Liang, Yong ^c,d   Morell, Robert J ^b   Touchman, Jeffrey W ^f,g   Lyons, Robert H ^h   Noben Trauth, Konrad ^b   Friedman, Thomas B ^b   Camper, Sally A ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

^d NIDCD   (United States)

^e UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^f NATIONAL INSTITUTES OF HEALTH   (United States)

^g NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^h UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN STEM RESPONSE; CYTOSKELETON; DNA SEQUENCE; GENE MUTATION; HAIR CELL; HEARING IMPAIRMENT; HISTOPATHOLOGY; MOUSE; NONHUMAN; PRIORITY JOURNAL; TRANSGENIC MOUSE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BRAIN; CHROMOSOMES, BACTERIAL; DEAFNESS; EAR, INNER; FEMALE; GENETIC COMPLEMENTATION TEST; HAIR CELLS; HUMANS; LIVER; MALE; MICE; MICE, INBRED C57BL; MICE, MUTANT STRAINS; MICE, TRANSGENIC; MYOSINS; PHENOTYPE; POINT MUTATION; TRANSGENES;

ANIMALIA; BACTERIA (MICROORGANISMS); MUS MUSCULUS;

EID: 17644442703     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5368.1444     Document Type: Article

References (37)

1. N. Dobrovolskaia-Zavasdkaia, Arch. Biol. 38, 457 (1928); G. D. Snell and L. W. Law, J. Hered. 30, 447 (1939).
2. N. Dobrovolskaia-Zavasdkaia, Arch. Biol. 38, 457 (1928); G. D. Snell and L. W. Law, J. Hered. 30, 447 (1939).
3. T. B. Friedman et al., Nature Genet. 9, 86 (1995).
4. Y. Liang et al., Am. J. Hum. Genet. 62, 904 (1998).
5. A. Sobin and A. Flock, Adv. Oto-Rhino-Laryngol. 25, 12 (1981); D. Kohrman, E. Lambert, D. V. Garrett, D. F. Dolan, Y. Raphael, paper presented at the 21st Midwinter Research Meeting of the Association for Research in Otolaryngology, St. Petersburg Beach, FL, 1998 (www.aro.org); A. Sobin, M. Anniko, A. Flock, Arch. Oto-Rhino-Laryngol. 236, 1 (1982).
6. A. Sobin and A. Flock, Adv. Oto-Rhino-Laryngol. 25, 12 (1981); D. Kohrman, E. Lambert, D. V. Garrett, D. F. Dolan, Y. Raphael, paper presented at the 21st Midwinter Research Meeting of the Association for Research in Otolaryngology, St. Petersburg Beach, FL, 1998 (www.aro.org); A. Sobin, M. Anniko, A. Flock, Arch. Oto-Rhino-Laryngol. 236, 1 (1982).
7. A. Sobin and A. Flock, Adv. Oto-Rhino-Laryngol. 25, 12 (1981); D. Kohrman, E. Lambert, D. V. Garrett, D. F. Dolan, Y. Raphael, paper presented at the 21st Midwinter Research Meeting of the Association for Research in Otolaryngology, St. Petersburg Beach, FL, 1998 (www.aro.org); A. Sobin, M. Anniko, A. Flock, Arch. Oto-Rhino-Laryngol. 236, 1 (1982).
8. Y. Wakabayashi et al., Biochem. Biophys. Res. Commun. 234, 107 (1997); F. Probst, Q. Xhao, K.-S. Chen, A. Wang, T. Friedman, J. Lupski, S. Camper, unpublished data.
9. Y. Wakabayashi et al., Biochem. Biophys. Res. Commun. 234, 107 (1997); F. Probst, Q. Xhao, K.-S. Chen, A. Wang, T. Friedman, J. Lupski, S. Camper, unpublished data.
10. R. K. Wilson and E. R. Mardis, in Analyzing DNA, B. Birren, E. D. Green, S. Klapholz, R. M. Myers, J. Roskams, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997), p. 398; S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J. Mol. Biol. 215, 403 (1990). SeqWright Inc. (Houston, TX) generated the M13 DNA shotgun library and approximately half of the sequence data. Sequence contig assembly and editing were performed with the Sequencher program (Gene Codes Corp., Ann Arbor, MI); the web sites for GRAIL and GENSCAN arc http://compbio.ornl.gov/Grail-bin/EmptyGrailForm and http://gnomic.stanford.edu/~chris/GENSCANW.html [C. Burge and S. Karlin, J. Mol. Biol. 268, 78 (1997)].
11. R. K. Wilson and E. R. Mardis, in Analyzing DNA, B. Birren, E. D. Green, S. Klapholz, R. M. Myers, J. Roskams, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997), p. 398; S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J. Mol. Biol. 215, 403 (1990). SeqWright Inc. (Houston, TX) generated the M13 DNA shotgun library and approximately half of the sequence data. Sequence contig assembly and editing were performed with the Sequencher program (Gene Codes Corp., Ann Arbor, MI); the web sites for GRAIL and GENSCAN arc http://compbio.ornl.gov/Grail-bin/EmptyGrailForm and http://gnomic.stanford.edu/~chris/GENSCANW.html [C. Burge and S. Karlin, J. Mol. Biol. 268, 78 (1997)].
12. R. K. Wilson and E. R. Mardis, in Analyzing DNA, B. Birren, E. D. Green, S. Klapholz, R. M. Myers, J. Roskams, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997), p. 398; S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J. Mol. Biol. 215, 403 (1990). SeqWright Inc. (Houston, TX) generated the M13 DNA shotgun library and approximately half of the sequence data. Sequence contig assembly and editing were performed with the Sequencher program (Gene Codes Corp., Ann Arbor, MI); the web sites for GRAIL and GENSCAN arc http://compbio.ornl.gov/Grail-bin/EmptyGrailForm and http://gnomic.stanford.edu/~chris/GENSCANW.html [C. Burge and S. Karlin, J. Mol. Biol. 268, 78 (1997)].
13. R. K. Wilson and E. R. Mardis, in Analyzing DNA, B. Birren, E. D. Green, S. Klapholz, R. M. Myers, J. Roskams, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997), p. 398; S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J. Mol. Biol. 215, 403 (1990). SeqWright Inc. (Houston, TX) generated the M13 DNA shotgun library and approximately half of the sequence data. Sequence contig assembly and editing were performed with the Sequencher program (Gene Codes Corp., Ann Arbor, MI); the web sites for GRAIL and GENSCAN arc http://compbio.ornl.gov/Grail-bin/EmptyGrailForm and http://gnomic.stanford.edu/~chris/GENSCANW.html [C. Burge and S. Karlin, J. Mol. Biol. 268, 78 (1997)].
14. V. Mermall, P.L. Post, M.S. Mooseker, Science 279, 527 (1998).
15. P. Linder Nature 337, 121 (1989); the consensus ATP-GTP binding site is (A/G)XXXXGK(S/T/G) and the Myo15 sequences are GESDSGKT, amino acids 132 to 139, and APPRLGKS at amino acids 566 to 573 (16). The Myo15 cDNA sequence has been deposited in GenBank (accession number AF053130).
16. A. Wang et al., Science 280, 1447 (1998).
17. F. J. Probst, R. A. Fridell, Y. Raphael, T. L. Saunders, A. Wang, Y. Liang, R. J. Morell, J. W. Touchman, R. H. Lyons, K. Noben-Trauth, T. B. Friedman, S. A. Camper, data not shown.
18. Myosin home page, Medical Research Council, UK (www.mrc-lmb.cam.ac.uk/myosin/myosin.html).
19. H. M. Warrick and J. A. Spudich, Annu. Rev. Cell. Biol. 3, 379 (1987); J. A. Porter and C. Montell, J. Cell Biol. 122, 601 (1993).
20. H. M. Warrick and J. A. Spudich, Annu. Rev. Cell. Biol. 3, 379 (1987); J. A. Porter and C. Montell, J. Cell Biol. 122, 601 (1993).
21. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
22. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
23. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
24. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
25. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
26. K. A. Avraham et al., Nature Genet. 11, 369 (1995); F. Gibson et al., Nature 374, 62 (1995); D. Weil et al., ibid., p. 60; X. Liu et al., Nature Genet. 16, 188 (1997); D. Weil et al., ibid., p. 191; C. Petit, ibid. 14, 385 (1996).
27. T. Self et al., Development 125, 557 (1998); K. Steel and K. Avraham, personal communication.
28. T. Self et al., Development 125, 557 (1998); K. Steel and K. Avraham, personal communication.
29. M. S. Deol, J. Genet. 52, 562 (1954).
30. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
31. Y. Raphael and R. A. Altschuler, Cell Motil. Cytoskeleton 18, 215 (1991); Y. Raphael, B. Athey, Y. Wang, M. K. Lee, R. A. Altschuler, Hear. Res. 76, 173 (1994).
32. Y. Raphael and R. A. Altschuler, Cell Motil. Cytoskeleton 18, 215 (1991); Y. Raphael, B. Athey, Y. Wang, M. K. Lee, R. A. Altschuler, Hear. Res. 76, 173 (1994).
33. W. F. Dietrich et al., Nature Genet. 7, 220 (1994).
34. B. Hogan, R. Beddington, F. Costantini, E. Lacey, Manipulating the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994).
35. J. D. Thompson, D. G. Higgins, T. J. Gibson, Nucleic Acids Res. 22, 4673 (1994); PHYLIP package, J. Felsenstein, University of Washington.
36. J. D. Thompson, D. G. Higgins, T. J. Gibson, Nucleic Acids Res. 22, 4673 (1994); PHYLIP package, J. Felsenstein, University of Washington.
37. All experiments involving animals were approved by the University of Michigan Committee on the Use and Care of Animals. Animals were housed in facilities accredited by the American Association for the Accreditation of Laboratory Animal Care. The university's Animal Welfare Assurance is on file with the NIH Office for Protection from Research Risks (A3114-01). All procedures are in compliance with guidelines and standards set by local, state, and federal agencies. Supported by NIDCD intramural research projects Z01 DC 00035, Z01 DC 00038, and Z01 DC 02407 (T.B.F.), and extramural grant R01DC 01634 (Y.R.), National Institute of Child Health and Human Development grant R01 HD30428 (S.A.C.), NSF (F.J.P.), and National Institute of General Medical Sciences grant T32 GM07863 (F.J.P.). We thank N. Dietrich, M. Fergusson, A. Gupta, E. Sorbello, R. Torkzadeh, C. Varner, M. Walker, G. Bouffard, and S. Beckstrom-Sternberg of the NIH Intramural Sequencing Center; C. Esposito, S. Genik, A. Thomas, and H. Luderer of the University of Michigan DNA Sequencing Core; P. Gillespie and M. Berard of the University of Michigan Transgenic Animal Model Core; L. Beyer, D. Dolan, K. Douglas, C. Edwards, E. Lambert, G. Levy, and P. Tucker of the University of Michigan; and J. Beals of PhotoGraphic for their contributions.