Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: Identification of enzymatic bottlenecks by systematic gene overexpression

Metabolic Engineering

Volumn 10, Issue 2, 2008, Pages 69-77

  Lütke Eversloh, Tina ^a   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Aromatic amino acid; Escherichia coli; Gene overexpression; L tyrosine; Metabolic engineering

Indexed keywords

BIOSYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; METABOLISM;

AROMATIC AMINO ACID; GENE OVEREXPRESSION; METABOLIC ENGINEERING;

AMINO ACIDS;

3 DEOXY 7 PHOSPHOHEPTULONATE SYNTHASE; AROMATIC AMINO ACID; AROMATIC AMINO ACID TRANSAMINASE; BACTERIAL PROTEIN; OXIDOREDUCTASE; PREPHENATE DEHYDROGENASE; PROTEIN YDIB; SHIKIMATE DEHYDROGENASE; SHIKIMATE KINASE; SHIKIMATE KINASE I; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CHEMICAL REACTION; COMBINATORIAL CHEMISTRY; ENZYME CHEMISTRY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE TARGETING; GENETIC CODE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 39849084210     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2007.12.001     Document Type: Article

References (33)

1. F. Bolivar R.L. Rodriguez P.J. Greene M.C. Betlach H.L. Heynecker H.W. Boyer Construction and characterization of new cloning vehicles. 2: multipurpose cloning system Gene 2 1977 95 113
2. J. Bongaerts M. Krämer U. Müller L. Raeven M. Wubbolts Metabolic engineering for microbial production of aromatic amino acids and derived compounds Metab. Eng. 3 2001 289 300
3. S.S. Chandran J. Yi K.M. Draths R. von Daeniken W. Weber J.W. Frost Phosphoenolpyruvate availability and the biosynthesis of shikimic acid Biotechnol. Prog. 19 2003 808 814
4. R.C. DeFeyter Shikimate kinases from Escherichia coli K12 Methods Enzymol. 142 1987 355 361
5. R.C. DeFeyter J. Pittard Purification and properties of shikimate kinase II from Escherichia coli K-12 J. Bacteriol. 165 1986 331 333
6. K.A. Dell J.W. Frost Identification and removal of impediments to biocatalytic synthesis of aromatics from D-glucose: rate-limiting enzymes in the common pathway of aromatic amino acid biosynthesis J. Am. Chem. Soc. 115 1993 11581 11589
7. B. Ely J. Pittard Aromatic amino acid biosynthesis: Regulation of shikimate kinase in Escherichia coli K-12 J. Bacteriol. 138 1979 933 943
8. J.W. Frost K.M. Draths Biocatalytic synthesis of aromatics from D-glucose: renewable microbial sources of aromatic compounds Annu. Rev. Mircobiol. 49 1995 557 579
9. Frost, J.W., Snell, K.D., Frost, K.M., 1998. Deblocking the common pathway of aromatic amino acid synthesis. United States Patent No. US 5,776,736.
10. M. Ikeda Amino acid production processes T. Scheper R. Faurie J. Thommel Advances in Biochemical Engineering/biotechnology vol. 79 2003 Springer Berlin, Heidelberg, New York 1 35
11. M. Ikeda Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering Appl. Microbiol. Biotechnol. 69 2006 615 626
12. H. Ito K. Sato H. Enei Y. Hirose Improvement in microbial production of L-tyrosine by gene dosage effect of aroL gene encoding shikimate kinase Agric. Biol. Chem. 54 1990 823 824
13. M. Krämer J. Bongaerts R. Bovenberg S. Kremer U. Müller S. Orf M. Wubbolts L. Raeven Metabolic engineering for microbial production of shikimic acid Metab. Eng. 5 2003 277 283
14. W. Leuchtenberger K. Huthmacher K. Drauz Biotechnological production of amino acids and derivatives: current status and prospects Appl. Microbiol. Biotechnol. 69 2005 1 8
15. 2 regulatory elements Nucleic Acids Res 25 1997 1203 1210
16. T. Lütke-Eversloh G. Stephanopoulos Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli : generation and characterization of tyrosine-insensitive mutants Appl. Environ. Microbiol. 71 2005 7224 7228
17. T. Lütke-Eversloh G. Stephanopoulos L-Tyrosine production by deregulated strains of Escherichia coli Appl. Microbiol. Biotechnol. 75 2007 103 110
18. T. Lütke-Eversloh G. Stephanopoulos A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates J. Ind. Microbiol. Biotechnol. 34 2007 807 811
19. T. Lütke-Eversloh C.N.S. Santos G. Stephanopoulos Perspectives of biotechnological production of L-tyrosine and its applications Appl. Microbiol. Biotechnol. 77 2007 751 762
20. G. Michel A.W. Roszak V. Sauvé J. Maclean A. Matte J.R. Coggins M. Cygler A.J. Lapthorn Structure of shikimate dehydrogenase AroE and its paralog YdiB J. Biol. Chem. 278 2003 19463 19472
21. F.C. Neidhardt P.L. Bloch D.F. Smith Culture medium for enterobacteria J. Bacteriol. 119 1974 736 747
22. M. Oldiges M. Kunze D. Degenring G.A. Sprenger R. Takors Stimulation, monitoring, and analysis of pathway dynamics by metabolic profiling in the aromatic amino acid pathway Biotechnol. Prog. 20 2004 1623 1633
23. M.M. Olson L.J. Templeton W. Suh P. Youderian F.S. Sariaslani A.A. Gatenby T.K. Van Dyk Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains Appl. Microbiol. Biotechnol. 74 2007 1031 1040
24. R. Patnaik J.C. Liao Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield Appl. Environ. Microbiol. 60 1994 3903 3908
25. R. Patnaik R.G. Spitzer J.C. Liao Pathway engineering for production of aromatics in Escherichia coli : confirmation of stoichiometric analysis by independent modulation of AroG, TktA and Pps activities Biotech. Bioeng. 46 1995 361 370
26. J. Pittard Biosynthesis of aromatic amino acids F.C. Neidhardt Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology vol. 1 1996 American Society of Microbiology Washington, DC 458 484
27. J. Sambrock E.F. Fritsch T. Maniatis Molecular Cloning: A Laboratory Manual second ed 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
28. Santos, C.N.S., Stephanopoulos, G., 2007. Methods for identifying bacterial strains that produce L-tyrosine. United States Patent Application No. US 60/965,149.
29. K.D. Snell K.M. Draths J.W. Frost Synthetic modification of the Escherichia coli chromosome: enhancing the biocatalytic conversion of glucose into aromatic chemicals J. Am. Chem. Soc. 118 1996 5605 5614
30. G.A. Sprenger From scratch to value: engineering Escherichia coli wild type cells to the production of L-phenylalanine and other fine chemicals derived from chorismate Appl. Microbiol. Biotechnol. 75 2007 739 749
31. Takai, A., Nishi, R., Joe, Y., Ito, H., 2005. L-tyrosine producing bacterium and a method for producing L-tyrosine. United States Patent No. 2005/0277179 A1.
32. Tribe, D.E., 1987. Novel microorganism and method. United States Patent No. US 4,681,852.
33. T.P. Waalkes S. Udenfriend A fluorometric method for the estimation of tyrosine in plasma and tissues J. Lab. Clin. Med. 50 1957 733 736