Genetic evidence for a predominant role of PI3Kβ catalytic activity in ITAM- and integrin-mediated signaling in platelets

Blood

Volumn 114, Issue 10, 2009, Pages 2193-2196

  Canobbio, Ilaria ^a   Stefanini, Lucia ^a,c   Cipolla, Lina ^a   Ciraolo, Elisa ^b   Gruppi, Cristian ^a   Balduini, Cesare ^a   Hirsch, Emilio ^b   Torti, Mauro ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

^c THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

15 HYDROXY 11ALPHA,9ALPHA EPOXYMETHANOPROSTA 5,13 DIENOIC ACID; ADENOSINE DIPHOSPHATE; FIBRINOGEN; G PROTEIN COUPLED RECEPTOR; GLYCOPROTEIN VI; INTEGRIN; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3 KINASE BETA; PHOSPHATIDYLINOSITOL 3 KINASE GAMMA; PROTEIN KINASE B; RAP1 PROTEIN; RAP1B PROTEIN; UNCLASSIFIED DRUG; COLLAGEN RECEPTOR GLYCOPROTEIN VI, MOUSE; FIBRINOGEN RECEPTOR; ISOENZYME; PURINERGIC P2 RECEPTOR; RAP PROTEIN; RAP1B PROTEIN, MOUSE; VASOCONSTRICTOR AGENT;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; FIBRINOGEN BLOOD LEVEL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; SIGNAL TRANSDUCTION; THROMBOCYTE; THROMBOCYTE ACTIVATION; THROMBOCYTE ADHESION; THROMBOCYTE AGGREGATION; ANIMAL; DRUG EFFECT; DRUG POTENTIATION; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; METABOLISM; MOUSE MUTANT; PHOSPHORYLATION; PHYSIOLOGY; THROMBOCYTE ADHESIVENESS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; 15-HYDROXY-11 ALPHA,9 ALPHA-(EPOXYMETHANO)PROSTA-5,13-DIENOIC ACID; AMINO ACID MOTIFS; ANIMALS; BLOOD PLATELETS; ENZYME ACTIVATION; FIBRINOGEN; ISOENZYMES; MICE; MICE, KNOCKOUT; PHOSPHORYLATION; PLATELET ADHESIVENESS; PLATELET AGGREGATION; PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX; PLATELET MEMBRANE GLYCOPROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; RAP GTP-BINDING PROTEINS; RECEPTORS, PURINERGIC P2; SIGNAL TRANSDUCTION; VASOCONSTRICTOR AGENTS;

EID: 70349251723     PISSN: 00064971     EISSN: 15280020     Source Type: Journal    
DOI: 10.1182/blood-2009-03-208074     Document Type: Article

References (19)

1. Jackson SP, Yap CL, Anderson KE. Phosphoinositide 3-kinases and the regulation of platelet function. Biochem Soc Trans. 2004;32(Pt 2):387-392.
2. Cosemans JM, Munnix IC, Wetzker R, Heller R, Jackson SP, Heemskerk JW. Continuous signaling via PI3K isoforms β and γ is required for platelet ADP receptor function in dynamic thrombus stabilization. Blood. 2006;108(9):3045-3052. (Pubitemid 44776943)
3. Hirsch E, Bosco O, Tropel P, et al. Resistance to thromboembolism in PI3Kγ-deficient mice. FASEB J. 2001;15(11):2019-2021.
4. Woulfe D, Jiang H, Mortensen R, Yang J, Brass LF. Activation of Rap1B by Gi family members in platelets. J Biol Chem. 2002;277(26):23382-23390. (Pubitemid 34952169)
5. Lova P, Paganini S, Hirsch E, et al. A selective role for phosphatidylinositol 3,4,5-trisphosphate in the Gi-dependent activation of platelet Rap1B. J Biol Chem. 2003;278(1):131-138. (Pubitemid 36043554)
6. Jackson SP, Schoenwaelder SM, Goncalves I, et al. PI 3-kinase p110β: a new target for antithrombotic therapy. Nat Med. 2005;11(5):507-514.
7. Patrucco E, Notte A, Barberis L, et al. PI3Kγ modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell. 2004;118(3):375-387. (Pubitemid 39061117)
8. Schoenwaelder SM, Ono A, Sturgeon S, et al. Identification of a unique co-operative phosphoinositide 3-kinase signaling mechanism regulating integrin αIIbβ3 adhesive function in platelets. J Biol Chem. 2007;282(39):28648-28658. (Pubitemid 47606062)
9. Pasquet JM, Bobe R, Gross B, et al. A collagenrelated peptide regulates phospholipase Cγ2 via phosphatidylinositol 3-kinase in human platelets. Biochem J. 1999;342(Pt 1):171-177. (Pubitemid 29410863)
10. Watanabe N, Nakajima H, Suzuki H, et al. Functional phenotype of phosphoinositide 3-kinase p85α-null platelets characterized by an impaired response to GP VI stimulation. Blood. 2003; 102(2):541-548. (Pubitemid 36841973)
11. Zhang J, Banfić H, Straforini F, Tosi L, Volinia S, Rittenhouse SE. A type II phosphoinositide 3-kinase is stimulated via activated integrin in platelets. A source of phosphatidylinositol 3-phosphate. J Biol Chem. 1998;273(23):14081-14084. (Pubitemid 28319113)
12. Lian L, Wang Y, Draznin J, et al. The relative role of PLCbβ and PI3Kγ in platelet activation. Blood. 2005;106(1):110-117. (Pubitemid 40967181)
13. Bi L, Okabe I, Bernard DJ, Nussbaum RL. Early embryonic lethality in mice deficient in the p110β catalytic subunit of PI 3-kinase. Mamm Genome. 2002;13(3):169-172. (Pubitemid 34185317)
14. Ciraolo E, Iezzi M, Marone R, et al. Phosphoinositide 3-kinase p110β activity: key role in metabolism and mammary gland cancer but not development. Sci Signal. 2008;1(36):ra3.
15. Bernardi B, Guidetti GF, Campus F, et al. The small GTPase Rap1b regulates the cross talk between platelet integrin α2β1 and integrin αIIbβ3. Blood. 2006;107(7):2728-2735.
16. Campus F, Lova P, Bertoni A, Sinigaglia F, Balduini C, Torti M. Thrombopoietin complements Gi- but not Gq-dependent pathways for integrin αIIbβ3 activation and platelet aggregation. J Biol Chem. 2005;280(26):24386-25243
17. Condliffe AM, Davidson K, Anderson KE, et al. Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils. Blood. 2005;106(4):1432-1440.
18. Larson MK, Chen H, Kahn ML, et al. Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets. Blood. 2003;101(4):1409-1415. (Pubitemid 36182513)
19. Guillermet-Guibert J, Bjorklof K, Salpekar A, et al. The p110β isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110γ. Proc Natl Acad Sci U S A. 2008;105(24):8292-8297. (Pubitemid 351904749)