Plasmid DNA production combining antibiotic-free selection, inducible high yield fermentation, and novel autolytic purification

Biotechnology and Bioengineering

Volumn 104, Issue 3, 2009, Pages 505-515

  Carnes, Aaron E ^a   Hodgson, Clague P ^a   Luke, Jeremy M ^a   Vincent, Justin M ^a   Williams, James A ^a  

^a NATURE TECHNOLOGY CORPORATION   (United States)

Author keywords

Autolysis; DNA vaccine; Escherichia coli; Gene therapy; Lysis; Plasmid

Indexed keywords

AUTOLYSIS; DNA VACCINE; GENE THERAPY; LYSIS; PLASMID;

ANTIGEN-ANTIBODY REACTIONS; CELL MEMBRANES; CYTOLOGY; DEBRIS; DNA; ESCHERICHIA COLI; FERMENTATION; GENETIC ENGINEERING; NUCLEIC ACIDS; PURIFICATION; VACCINES;

GENES;

BACTERIAL PROTEIN; DETERGENT; ENDOLYSIN; PEPTIDOGLYCAN; PLASMID DNA; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; BACTERIAL CELL WALL; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIOPHAGE LAMBDA; CATALYSIS; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; EXTRACTION; FERMENTATION; HOST; HUMAN; HUMAN CELL; NONHUMAN; PROMOTER REGION; PURIFICATION; QUANTUM YIELD;

BACTERIOLYSIS; BIOTECHNOLOGY; DNA; ENDOPEPTIDASES; ESCHERICHIA COLI; FERMENTATION; PLASMIDS; SELECTION (GENETICS); VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 70249111699     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22415     Document Type: Article

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