A continuous process to extract plasmid DNA based on alkaline lysis

Nature Protocols

Volumn 3, Issue 2, 2008, Pages 176-180

  Li, Xiaolin ^a   Jin, Huali ^a   Wu, Zhifang ^a   Rayner, Simon ^a,b   Wang, Bin ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; DNA VACCINE; PLASMID DNA;

ALKALINE LYSIS; ALKALINITY; ARTICLE; BACTERIUM CULTURE; CENTRIFUGATION; CONTINUOUS PROCESS; DNA EXTRACTION; DNA IMMUNIZATION; DNA PURIFICATION; ESCHERICHIA COLI; NONHUMAN; NONVIRAL GENE THERAPY; PLASMID; PRECIPITATION; PRIORITY JOURNAL; RNA INTERFERENCE; SCALE UP;

DNA, BACTERIAL; ESCHERICHIA COLI; FILTRATION; INDUSTRIAL MICROBIOLOGY; PLASMIDS; SODIUM HYDROXIDE;

ESCHERICHIA COLI;

EID: 39549096885     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.526     Document Type: Article

References (23)

1. Friedmann, T. The road toward human gene therapy-a 25-year perspective. Ann. Med. 29, 575-577 (1997).
2. Liljeqvist, S. & Stahl, S. Production of recombinant subunit vaccines: protein immunogens, live delivery systems and nucleic acid vaccines. J. Biotechnol. 73, 1-33 (1999).
3. Crystal, R.G. The gene as the drug. Nat. Med. 1, 15-17 (1995).
4. Ferreira, G.N., Monteiro, G.A., Prazeres, D.M. & Cabral, J.M. Downstream processing of plasmid DNA forgene therapy and DNA vaccine applications. Trends Biotechnol. 18, 380-388 (2000).
5. Levy, M.S., O'Kennedy, R.D., Ayazi-Shamlou, P. & Dunnill, P. Biochemical engineering approaches to the challenges of producing pure plasmid DNA. Trends Biotechnol. 18, 296-305 (2000).
6. Eastman, E.M. & Durland, R.H. Manufacturing and quality control of plasmid-based gene expression systems. Adv. Drug Deliv. Rev. 30 33-48 (1998).
7. Prather, K.J., Sagar, S., Murphy, J. & Chartrain, M. Industrial scale production of plasmid DNA for vaccine and gene therapy: Plasmid design, production, and purification. Enzyme Microb. Technol. 33, 865-883 (2003).
8. Prazeres, D.M., Ferreira, G.N., Monteiro, G.A., Cooney, C.L. & Cabral, J.M. Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: Problems and bottlenecks. Trends Biotechnol. 17, 169-174 (1999).
9. Birnboim, H.C. & Doly, J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513-1523 (1979).
10. Kelly, B. & Hatton, T. The fermentation/downstream processing interface. Bioseparation 1, 333-349 (1991).
11. Prazeres, D.M., Schluep, T. & Cooney, C. Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J. Chromatogr. A 806, 31-45 (1998).
12. Theodossiou, I., Collins, I.J., Ward, J.M., Thomas O.R.T. & Dunnill, P. The processing of a plasmid-based gene from E. coli. Primary recovery by filtration. Bioprocess Eng. 16, 175-183 (1997).
13. Sambrook, J. & Russell, D.W. Molecular Cloning: A Laboratory Manual 3rd edn. (Cold Spring Press, New York, 2001).
14. Wang, B., Merva, M., Williams, W.V. & Weiner, D.B. Large-scale preparation of plasmid DNA by microwave lysis. Biotechniques 18 554-555 (1995).
15. Zhu, K., Jin, H., He, Z., Zhu, O. & Wang, B. A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis. Nat. Protoc. 1, 3088-3093 (2006).
16. Zhu, K. et al. A continuous thermal lysis procedure for the large-scale preparation of plasmid DNA. J. Biotechnol. 118, 257-264 (2005).
17. Li, X. et al. An automated process to extract plasmid DNA by alkaline lysis. Appl. Microbiol Biotechnol. 75, 1217-1223 (2007).
18. Hoopes, B.C. & McClure, W.R. Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Res. 9, 5493-5504 (1981).
19. Gustincich, S., Manfioletti, G., Del Sal, G., Schneider, C. & Caminci, P. A fast method for high-quality genomic DNA extraction from whole human blood. Biotechniques 11, 298-300, 302 (1991).
20. Ishaq, M., Wolf, B. & Ritter, C. Large-scale isolation of plasmid DNA using cetyltrimethylammonium bromide. Biotechniques 9, 19-20, 22, 24 (1990).
21. Davies, M.J. et al. Improved manufacture and application of an agarose magnetizable solid-phase support. Appl. Biochem. Biotechnol. 68, 95-112 (1997).
22. Jin, H. et al. Effect of chemical adjuvants on DNA vaccination. Vaccine 22, 2925-2935 (2004).
23. Sayers, J.R., Evans, D. & Thomson, J.B. Identification and eradication of a denatured DNA isolated during alkaline lysis-based plasmid purification procedures. Anal. Biochem. 241, 186-189 (1996).