Impact of engineering flow conditions on plasmid DNA yield and purity in chemical cell lysis operations

Biotechnology and Bioengineering

Volumn 87, Issue 3, 2004, Pages 293-302

  Meacle, Francis J ^a   Lander, R ^a   Ayazi Shamlou, P ^b   Titchener Hooker, N J ^b  

^a MERCK RESEARCH LABORATORIES   (United States)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Alkaline lysis; Gene therapy; Plasmid; Shear

Indexed keywords

BACTERIA; CONTAMINATION; DNA; MOLECULAR WEIGHT; PH EFFECTS;

ALKALINE SOLUTIONS; CELLULAR COMPONENTS; FLOW CONDITIONS;

BIOTECHNOLOGY;

DNA FRAGMENT; PLASMID DNA; PROTEINASE K; SINGLE STRANDED DNA; SODIUM HYDROXIDE;

ALKALINITY; ARTICLE; BACTERIAL CELL; BIOENGINEERING; CELL COMPOSITION; CHROMOSOME; CONCENTRATION RESPONSE; CYTOLYSIS; DNA DENATURATION; DNA STRAND BREAKAGE; ESCHERICHIA COLI; GENE THERAPY; NONHUMAN; PH MEASUREMENT; SHEAR FLOW;

BIOMEDICAL ENGINEERING; CELL EXTRACTS; CELL FRACTIONATION; CHROMATOGRAPHY; DNA, BACTERIAL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MICROFLUIDICS; PLASMIDS; SHEAR STRENGTH; SODIUM HYDROXIDE;

BACTERIA (MICROORGANISMS);

EID: 4043124016     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20114     Document Type: Article

References (22)

1. Bimboim H, Doly J. 1979. A rapid alkaline lysis procedure for screening recombinant plasmid DNA. J Nucl Acids Res 7:1513-1522.
2. Chamsart S, Patel H, Hanak JAJ, Hitchcock AG, Nienow AW. 2001. The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products. Biotechnol Bioeng 75(4):387-392.
3. Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM, Dunnill P. 1998. Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release. Biotechnol Bioeng 60(6):768-770.
4. Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ. 2002. A mass balance study to assess the extent of contaminant removal achieved in the operations for the primary recovery of plasmid DNA from Escherichia coli cells. Biotechnol Bioeng 77(7):796-805.
5. Davison PF. 1959. The effect of hydrodynamic shear on the deoxyribonucleic acid from T2 and T4 bacteriophages. Biochemistry 45: 1560-1568.
6. Ferreira GNM, Cabral JMS, Prazeres DMF. 1999. Monitoring of process streams in the large-scale production and purification of plasmid DNA for gene therapy applications. Pharma Pharmacol Comm 5:57-59.
7. Harnby N, Edwards M, Nienow A. 1992. Mixing in the process Industries. London: Butterworth Heinemann.
8. Levy MS, Ciccolini LAS, Yim S, Tsai T, Titchener-Hooker NJ, Shamlou PA, Dunnill P. 1999a. The effects of material properties and fluid flow intensity on plasmid DNA recovery during cell lysis. Chem Eng Sci 54(14):3171-3178.
9. Levy MS, Collins IJ, Yim SS, Ward JM, Titchener-Hooker N, Shamlou PA, Dunnill P. 1999b. Effect of shear on plasmid DNA in solution. Bioprocess Eng 20:7-13.
10. Levy MS, O'Kennedy R, Ayazi Shamlou P, Dunnill P. 2000. Biochemical engineering approaches to the challenges of producing pure plasmid DNA. Tibtech 18:296-305.
11. Liu MA. 2003. DNA vaccines: A review. J Int Med 253:302-410.
12. Meacle F. 2003. An experimental and computational fluid dynamics study of the influence of fluid mixing and fluid stress on DNA purification. Ph.D. thesis, University College, London, UK.
13. Mhashilkar A, Chada S, Roth J, Ramesh R. 2001. Gene therapy therapeutic approaches and implications. Biotechnol Adv 19:279-297.
14. Nguyen TQ, Kausch HH. 1992. Mechanochemical degradation in transient elongational flow. Adv Polymer Sci 100:74-182.
15. Prazeres DMF, Ferreira GNM, Monteiro GA, Cooney CL, Cabral JMS. 1999. Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: Problems and bottlenecks. Tibtech 17:169-174.
16. Prazeres DMF, Schluep T, Cooney CL. 1998. Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J Chromatogr A 806:31-45.
17. Qiagen Plasmid Mega and Giga Protocol. 1998. Qiagen Plasmid Purification Handbook 11/98:17-18.
18. Schlichting H. 1979. Boundary layer theory, 7th ed. New York: McGraw-Hill.
19. Thatcher D, Hitchcock A, Hanak J, Varley D. 1997. Method of plasmid DNA production and purification. Patent Application WO 97/29190.
20. Wan NC, McNeilly DS, Christopher CW. 1997. Method for lysing cells. U.S. Patent 5,837,529.
21. Wang Z, Le G, Shi Y, Wegrzyn G. 2002. Studies on recovery plasmid DNA from Echerichia coli by heat treatment. Proc Biochem 38:199-206.
22. Yim S, Shamlou P. 2000. The engineering effects of fluids flow on freely suspended biological macro-materials and macromolecules. Adv Biochem Eng/Biotechnol 67:83-122.