Analysis and use of endogenous nuclease activities in Escherichia coil lysates during the primary isolation of plasmids for gene therapy

Biotechnology and Bioengineering

Volumn 66, Issue 3, 1999, Pages 189-194

  Monteiro, G A ^a,b   Ferreira, G N M ^a   Cabral, J M S ^a   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b UNIVERSIDADE LUSÓFONA DE HUMANIDADES E TECNOLOGIAS   (Portugal)

Author keywords

Gene therapy; Nuclease; Plasmid; RNA degradation; RNase

Indexed keywords

BIODEGRADATION; CATALYST ACTIVITY; DNA; ENZYME KINETICS; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMPURITIES; PURIFICATION; RNA;

GENE THERAPY; LYSATES; NUCLEASES; PLASMIDS;

GENETIC ENGINEERING;

NUCLEASE; RIBONUCLEASE;

ARTICLE; COST; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ISOLATION; GENE THERAPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PLASMID; RNA DEGRADATION; VALIDATION PROCESS;

BIOTECHNOLOGY; DEOXYRIBONUCLEASES; DNA, BACTERIAL; ESCHERICHIA COLI; GENE THERAPY; HUMANS; PLASMIDS; RIBONUCLEASES; RNA, BACTERIAL;

EID: 0033454713     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(1999)66:3<189::AID-BIT7>3.0.CO;2-Z     Document Type: Article

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