A continuous thermal lysis procedure for the large-scale preparation of plasmid DNA

Journal of Biotechnology

Volumn 118, Issue 3, 2005, Pages 257-264

  Zhu, Kaichun ^a,b   Jin, Huali ^a   Ma, Yijie ^a   Ren, Zhihui ^b   Xiao, Chong ^a   He, Zhonghuai ^a   Zhang, Fuchun ^b   Zhu, Qinghong ^a   Wang, Bin ^a,b  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b XINJIANG UNIVERSITY   (China)

Author keywords

Continuous boiling lysis; Large scale; Plasmid

Indexed keywords

BACTERIA; BATCH CELL CULTURE; DRUG THERAPY; ELECTRIC COILS; ESCHERICHIA COLI; EXTRACTION; GENES; HEAT EXCHANGERS; THERMOANALYSIS; VACCINES;

BOILING LYSIS; PLASMID EXTRACTION; SCALEABLE PROCESS; STREAMLINE PROTOCOL;

DNA;

LYSOZYME; PLASMID DNA; PROTEIN VP1; VACCINE;

ANIMAL EXPERIMENT; ARTICLE; BACTERIOLYSIS; BACTERIUM CULTURE; BATCH FERMENTATION; CENTRIFUGATION; CONTROLLED STUDY; DNA EXTRACTION; DNA MODIFICATION; DNA SYNTHESIS; DNA VECTOR; ESCHERICHIA COLI; FERMENTATION; LYSIS; MOUSE; NONHUMAN; PERISTALTIC PUMP; PRIORITY JOURNAL; SUPERNATANT; THERMODYNAMICS;

CELL CULTURE TECHNIQUES; CELL FRACTIONATION; DNA, BACTERIAL; ESCHERICHIA COLI; HEAT; PLASMIDS;

ESCHERICHIA COLI;

EID: 22544464723     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.05.003     Document Type: Article

References (26)

1. H. Birnboim, and J. Doly A rapid alkaline extraction procedure for screening recombinant plasmid DNA Nucleic Acids Res. 7 1979 1513 1523
2. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
3. L.A. Ciccolini Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release Biotechnol. Bioeng. 60 1998 768 770
4. M. Clemson, and W. Kelly Optimizing alkaline lysis for DNA plasmid recovery Biotechnol. Appl. Biochem. 37 2003 235 244
5. R.G. Crystal The gene as the drug Nat. Med. 1 1995 15 17
6. R. Durland, and E. Eastman Manufacturing and quality control of plasmid-based gene expression systems Adv. Drug Deliv. Rev. 30 1998 33 48
7. G. Ferreia, G. Monteiro, D. Prazeres, and J. Cabral Downstream processing of plasmid DNA for gene therapy and DNA vaccine application Trends Biotech. 18 9 2000 380 388
8. F. Herrmann Clinical application of gene transfer J. Mol. Med. 74 1996 213 221
9. Hoisington, D., Khairallah, M., González-De-León, D., 1994. Laboratory Protocols: Applied Molecular Genetics Laboratory, Mexico, CIMMYT.
10. D. Holmes, and M. Quigley A rapid boiling method for the preparation of bacterial plasmids Anal. Biochem. 114 1981 193 197
11. H. Jin, Y. Li, Z. Ma, F. Zhang, Q. Xie, D. Gu, and B. Wang Effect of chemical adjuvants on DNA vaccination Vaccine 22 2004 2925 2935
12. B. Kelly, and T. Hatton The fermentation/downstream pro-cessing interface Bioseparation 1 1991 333 349
13. R. Lahijani, G. Hulley, G. Soriano, N.A. Horn, and M. Marquet High-yield production of pBR322-derived plasmids intended for human gene therapy by employing a temperature-controllable point mutation Hum. Gene Ther. 7 1996 1971 1980
14. R. Lander, M. Winters, F. Meacle, B. Buckland, and A. Lee Fractional precipitation of plasmid DNA from lysate by CTAB Biotechnol. Bioeng. 79 2004 776 784
15. Lee, A.L., Sagar, S., 1996. A method for large scale plasmid purification. Patent Application. US, Merck Co.
16. M. Levy Effect of shear on plasmid DNA solution Bioprocess Eng. 20 1999 7 13
17. M. Levy, R.D. O'Kennedy, P. Ayazi Shamlou, and P. Dunnill Biochemical engineering approaches to the challenges of producing pure plasmid DNA Trends Biotechnol. 18 2000 296 305
18. D. Luo, and W. Saltzman Synthetic DNA delivery systems Nat. Biotech. 18 2000 33 37
19. A. Mountain Gene therapy: the first decade Trends Biotechnol. 18 2000 119 128
20. K. Prather, S. Sagar, J. Murphy, and M. Chartrain Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification Enz. Microbial Technol. 33 2003 865 883
21. D. Prazeres Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography J. Chromatogr. 806 1998 31 45
22. D.M.F. Prazeres Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks Trends Biotechnol. 17 1999 169 174
23. J. Sambrook, E. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press New York
24. M. Schleef Issues of large-scale plasmid DNA manufacturing A. Mountain U. Ney D. Schomburg Biotechnology 2nd ed. 1999 Wiley-VCH New York
25. I. Theodossiou The processing of plasmid based gene from Escherichia coli: primary recovery by filtration Bioprocess Eng. 16 1997 175 183
26. B. Wang, M. Merva, W. Williams, and D. Weiner Large-scale preparation of plasmid DNA by microwave lysis Biotechniques 18 1995 554 555