Application of a short intracellular pH method to flow cytometry for determining Saccharomyces cerevisiae vitality

Applied and Environmental Microbiology

Volumn 75, Issue 17, 2009, Pages 5615-5620

  Weigert, Claudia ^a   Steffler, Fabian ^a   Kurz, Tomas ^b   Shellhammer, Thomas H ^c   Methner, Frank Jürgen ^d  

^a Central Laboratory   (Germany)

^b Oregon State University ^*  (Germany)

^c OREGON STATE UNIVERSITY   (United States)

^d TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYFLUORESCEIN; DEAD CELLS; EXPONENTIAL PHASE; FLUORESCENT PROBES; GROWTH PHASIS; INTRACELLULAR PH; PH-DEPENDENT; PHOTOMETRIC MEASUREMENTS; ROUTINE ANALYSIS; SACCHAROMYCES CEREVISIAE; STATIONARY PHASE; STATIONARY PHASIS; UNIMODAL; VIABLE CELLS;

CHROMATOGRAPHIC ANALYSIS; FLOW CYTOMETRY; FLUORESCENCE; PHOTOMETERS; PHOTOMETRY; YEAST;

INDUCTIVELY COUPLED PLASMA;

6 CARBOXYFLUORESCEIN;

FLOW CYTOMETRY; GROWTH CURVE; PH; PHOTOMETER; SUBPOPULATION; VIABILITY; YEAST;

ACIDITY; ARTICLE; CELL PH; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUOROPHOTOMETRY; FUNGUS GROWTH; NONHUMAN; PHOTOMETRY; POPULATION; SACCHAROMYCES CEREVISIAE; YEAST;

CYTOSOL; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; SACCHAROMYCES CEREVISIAE; STAINING AND LABELING;

SACCHAROMYCES CEREVISIAE;

EID: 69449103461     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00650-09     Document Type: Article

References (39)

1. Back, W., T. Imai, C. Forster, and L. Narziss. 1998. Hefevitalität und Bierqualität. Monatsschrift. Brauwissenschaft. 11/12:189-195.
2. Blomberg, A. 2000. Metabolic surprises in Saccharomyces cerevisiae during adaptation to saline conditions: questions, some answers and a model. FEMS Microbiol. Lett. 182:1-8.
3. Boulton, C., W. Box, D. Quain, and S. Molzahn. 2001. Vicinal diketone reduction as a measure of yeast vitality. Tech. Q. Master Brew. Assoc. Am. 2:89-93.
4. +-ATPase. J. Microbiol. Methods 31:113-125.
5. Breeuwer, P., J. L. Drocourt, F. M. Rombouts, and T. Abee. 1994. Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry. Appl. Environ. Microbiol. 60:1467-1472.
6. Breeuwer, P., J. L. Drocourt, N. Bunschoten, M. H. Zwietering, F. M. Rombouts, and T. Abee. 1995. Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product. Appl. Environ. Microbiol. 61:1614-1619.
7. Breeuwer, P., and T. Abee. 2000. Assessment of the intracellular pH of immobilized and continuously perfused yeast cells employing fluorescence ratio imaging analysis. J. Microbiol. Methods 39:253-264. (Pubitemid 30004046)
8. Chow, S., D. Hedley, and I. Tannock. 1996. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases. Cytometry A 24:360-367. (Pubitemid 26285616)
9. Cimprich, P., J. Slavík, and A. Kotyk. 1995. Distribution of individual cytoplasmic pH values in a population of the yeast Saccharomyces cerevisiae. FEMS Microbiol. Lett. 130:245-251.
10. Dinsdale, M. G., D. Lloyd, P. McIntyre, and B. Jarvis. 1999. Yeast vitality during cider fermentation: assessment by energy metabolism. Yeast 15:285-293.
11. Ferguson, S. B., E. S. Anderson, R. B. Harshaw, T. Thate, N. L. Craig, and H. C. M. Nelson. 2005. Protein kinase A regulates constitutive expression of small heat-shock genes in an Msn2/4p-independent and Hsf1p-dependent manner in Saccharomyces cerevisiae. Genetics 169:1203-1214.
12. Fernández, S., G. González, and A. Sierra. 1991. The acidification power test and the behavior of yeast in brewery fermentations. Tech. Q. Master Brew. Assoc. Am. 28:89-95.
13. Fritsch, M., J. Starruß, A. Loesche, S. Mueller, and T. Bley. 2005. Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry. Biotechnol. Bioeng. 92:635-642.
14. Hewitt, C. J., and G. Nebe-Von-Caron. 2001. An industrial application of multiparameter flow cytometry: assessment of cell physiological state and its application to the study of microbial fermentations. Cytometry 44:179-187.
15. Hutter, K.-J. 2002. Flow cytometry: a new tool for direct control of fermentation processes. J. Inst. Brew. 108:48-51.
16. Hutter, K. 2002. Flow cytometric determinations of glycogen content of yeast during fermentation. J. Inst. Brew. 108:52-53.
17. Hutter, K., and F. Nitzsche. 2002. Untersuchungen über die Alterung der Bierhefen mit Hilfe der flusszytometrischen Analyse. Monatsschrift. Brauwissenschaft. 9/10:196-199.
18. Hutter, K., C. Kliem, F. Nitzsche, and M. Wießler. 2003. Biomonitoring der Betriebshefen in praxi mit fluoreszenzoptischen Verfahren. IX. Mitteilung: Trehalose-Stressprotektant der Saccharomyces-Hefen. Monatsschrift. Brauwissenschaft. 7/8:121-125.
19. Imai, T., I. Nakajima, and T. Ohno. 1994. Development of a new method for evaluation of yeast vitality by measuring intracellular pH. J. Am. Soc. Brew. Chem. 52:5-8.
20. Imai, T., and T. Ohno. 1995. Measurement of yeast intracellular pH by image processing and the change it undergoes during growth phase. J. Biotechnol. 38:165-172.
21. Imai, T., and T. Ohno. 1995. The relationship between viability and intracellular pH in the yeast Saccharomyces cerevisiae. Appl. Environ. Microbiol. 61:3604-3608.
22. Kara, B. V., W. Simpson, and J. R. M. Hammond. 1988. Prediction of the fermentation performance of brewing yeast with the acidification power test. J. Inst. Brew. 94:153-158.
23. Madshus, I. H. 1988. Regulation of intracellular pH in eukaryotic cells. Biochem. J. 250:1-8.
24. Müller, S., K.-J. Hutter, T. Bley, L. Petzold, and W. Babel. 1997. Dynamics of yeast cell states during proliferation and nonproliferation periods in a brewing reactor monitored by multidimensional flow cytometry. Bioprocess Biosyst. Eng. 17:287-293.
25. Nagar-Legmann, R., and P. Margalith. 1986. A comparative study of the lipid composition of yeasts with different fermentative capacities. Appl. Environ. Microbiol. 26:49-54.
26. Nagodawithana, T. W., and K. H. Steinkraus. 1976. Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in "rapid fermentation." Appl. Environ. Microbiol. 31:158-162.
27. Peddie, F. L., W. J. Simpson, B. V. Kara, S. C. Robertson, and J. R. M. Hammond. 1991. Measurement of endogenous oxygen uptake rates of brewers' yeast. J. Inst. Brew. 97:21-25.
28. Reed, R. H., J. A. Chudek, R. Foster, and G. M. Gadd. 1987. Osmotic significance of glycerol accumulation in exponentially growing yeasts. Appl. Environ. Microbiol. 53:2119-2123.
29. Rep, M., M. Krantz, J. M. Thevelein, and S. Hohmann. 2000. The transcriptional response of Saccharomyces cerevisiae to osmotic shock. J. Biol. Chem. 275:8290-8300.
30. Rodrigues, P. G., A. A. Barros, J. A. Rodrigues, A. A. Ferreira, C. Gonçalves, and J. R. M. Hammond. 2004. Vital titration: a new method for assessment of yeast vitality. Tech. Q. Master Brew. Assoc. Am. 41:277-281.
31. Slavík, J. 1982. Intracellular pH of yeast cells measured with fluorescent probes. FEBS Lett. 140:22-25.
32. Smart, K. 2003. Brewing yeast fermentation performance, 2nd ed. Blackwell Publishers, Oxford, United Kingdom.
33. Thiele, F., and W. Back. 2005. Measurement of yeast vitality using a modified version of the intracellular pH measurement (ICP). Monatsschrift. Brauwissenschaft. 1/2:2-5.
34. Trotter, E. W., L. Berenfeld, S. A. Krause, G. A. Petsko, and J. V. Gray. 2001. Protein misfolding and temperature up-shift cause G1 arrest via a common mechanism dependent on heat shock factor in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 98:7313-7318.
35. Valli, M., M. Sauer, P. Branduardi, N. Borth, D. Porro, and D. Mattanovich. 2005. Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry. Appl. Environ. Microbiol. 71:1515-1521.
36. Valli, M., M. Sauer, P. Branduardi, N. Borth, D. Porro, and D. Mattanovich. 2006. Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH. Appl. Environ. Microbiol. 72:5492-5499.
37. Visser, J. M., A. A. M. Jongeling, and H. J. Tanke. 1979. Intracellular pH-determination by fluorescence measurements. J. Histochem. Cytochem. 27:32-35.
38. Wheatcroft, R., Y. H. Lim, D. B. Hawthorne, B. J. Clarke, and T. E. Kavanagh. 1988. An assessment of the use of specific oxygen uptake measurements to predict the fermentation performance of brewing yeast. Proc. Int. Conv. Inst. Brew. 20:193-199.
39. Wieder, E. D., H. Hang, and M. H. Fox. 1993. Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1. Cytometry Part A 14:916-921. (Pubitemid 23323972)