Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. I. Fermentation, biohydrogenation, and microbial protein synthesis

Journal of Dairy Science

Volumn 92, Issue 8, 2009, Pages 3849-3860

  Karnati, S K R ^a   Sylvester, J T ^a,b   Ribeiro, C V D M ^a   Gilligan, L E ^a   Firkins, J L ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b Buckeye Nutrition   (United States)

Author keywords

Bromoethanesulfonate; Methane; Monensin; Protozoa

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); PROKARYOTA; PROTOZOA;

EID: 68949218347     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2008-1436     Document Type: Article

References (61)

1. Abel, H., B. Schröder, P. Lebzien, and G. Flachowsky. 2006. Effect of defaunation on fermentation characteristics and biotin balance in an artificial rumen-simulation system (RUSITEC) receiving diets with different amounts and types of cereal. Br. J. Nutr. 95:99-104.
2. AOAC. 1990. Official Methods of Analysis. 15th ed. AOAC Int. Arlington, VA.
3. Beauchemin, K. A., M. Kreuzer, F. O. O'Mara, and T. A. McAllister. 2008. Nutritional management for enteric methane abatement: A review. Aust. J. Exp. Agric. 48:21-27.
4. Boeckaert, C., V. Fievez, D. Van Heck, W. Verstraete, and N. Boon. 2007. Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle. Eur. J. Lipid Sci. Technol. 109:767-777.
5. Brown, M. S., C. H. Ponce, and R. Pulikanti. 2006. Adaptation of beef cattle to high-concentrate diets: Performance and ruminal metabolism. J. Anim. Sci. 84(E Suppl.):E25-E33.
6. Chaney, A. L., and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
7. Cotta, M., and R. Forster. 2006. The family Lachnospiraceae, including the genera Butyrivibrio, Lachnospira and Roseburia. Prokaryotes 4:1002-1021.
8. Dehority, B. A. 1993. Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa. CRC Press Inc., Boca Raton, FL.
9. Dehority, B. A. 2003. Rumen Microbiology. Nottingham University Press, Nottingham, UK.
10. Devillard, E., F. M. McIntosh, C. J. Newbold, and R. J. Wallace. 2006. Rumen ciliate protozoa contain high concentrations of conjugated linoleic acids and vaccenic acid, yet do not hydrogenate linoleic acid or desaturate stearic acid. Br. J. Nutr. 96:697-704.
11. Dohme, F., A. Machmüller, A. Wasserfallen, and M. Kreuzer. 2001. Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets. Lett. Appl. Microbiol. 32:47-51.
12. Eugène, M., H. Archimède, and D. Sauvant. 2004. Quantitative meta- analysis on the effects of defaunation of the rumen on growth, intake and digestion in ruminants. Livest. Prod. Sci. 85:81-97.
13. Fellner, V., F. D. Sauer, and J. K. G. Kramer. 1997. Effect of nigericin, monensin, and tetronasin on biohydrogenation in continuous flow- through ruminal fermenters. J. Dairy Sci. 80:921-928.
14. Firkins, J. L. 1996. Maximizing microbial protein synthesis in the rumen. J. Nutr. 126:1347S-1354S.
15. Firkins, J. L., A. N. Hristov, M. B. Hall, G. A. Varga, and N. R. St-Pierre. 2006. Integration of ruminal metabolism in dairy cattle. J. Dairy Sci. 89(E Suppl.):E31-E51.
16. Firkins, J. L., S. K. R. Karnati, and Z. Yu. 2008. Linking rumen function to animal response by application of metagenomics techniques. Aust. J. Exp. Agric. 48:711-721.
17. Firkins, J. L., and Z. Yu. 2006. Characterisation and quantification of the microbial populations in the rumen. Pages 19-54 in Ruminant Physiology, Digestion, Metabolism and Impact of Nutrition on Gene Expression, Immunology and Stress. K. Sejrsen, T. Hvelplund, and M. O. Nielsen, ed. Wageningen Academic Publishers, Wageningen, the Netherlands.
18. Firkins, J. L., Z. Yu, and M. Morrison. 2007. Ruminal nitrogen metabolism: Perspectives for integration of microbiology and nutrition for dairy. J. Dairy Sci. 90(E Suppl.):E1-E16.
19. Hall, M. B., and P. J. Weimer. 2007. Sucrose concentration alters fermentation kinetics, products, and carbon fates during in vitro fermentation with mixed ruminal microbes. J. Anim. Sci. 85:1467-1478.
20. Harfoot, C. G., and G. P. Hazlewood. 1997. Lipid metabolism in the rumen. Pages 382-426 in The Rumen Microbial Ecosystem. P. N. Hobson and C. S. Stewart, ed. Blackie Academic and Professional, New York, NY.
21. Harvatine, D. I., J. E. Winkler, M. Devant-Guille, J. L. Firkins, N. R. St-Pierre, and M. L. Eastridge. 2002. Whole linted cottonseed as a forage substitute: Fiber effectiveness and digestion kinetics. J. Dairy Sci. 85:1988-1999.
22. Hegarty, R. S. 1999. Reducing rumen methane emissions through elimination of rumen protozoa. Aust. J. Agric. Res. 50:1321-1327.
23. Hoover, W. H., B. A. Crooker, and C. J. Sniffen. 1976. Effects of differential solid-liquid removal rates on protozoa numbers in continuous cultures of rumen contents. J. Anim. Sci. 43:528-534.
24. Hristov, A. N., and J.-P. Jouany. 2005. Factors affecting the efficiency of nitrogen utilization in the rumen. Pages 117-166 in Nitrogen and Phosphorus Nutrition of Cattle and Environment. A. N. Hristov and E. Pfeffer, ed. CAB International, Wallingford, UK.
25. Ipharraguerre, I. R., and J. H. Clark. 2003. Usefulness of ionophores for lactating dairy cows: A review. Anim. Feed Sci. Technol. 106:39-57.
26. Jenkins, T. C., V. Fellner, and R. K. McGuffey. 2003. Monensin by fat interactions on trans fatty acids in cultures of mixed ruminal microorganisms grown in continuous fermenters fed corn or barley. J. Dairy Sci. 86:324-330.
27. Jenkins, T. C., R. J. Wallace, P. J. Moate, and E. E. Mosley. 2008. Board-Invited Review: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J. Anim. Sci. 86:397-412.
28. Johnson, K. A., and D. E. Johnson. 1995. Methane emissions from cattle. J. Anim. Sci. 73:957-963.
29. Karnati, S. K. R., Z. Yu, and J. L. Firkins. 2009. Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. II. Interaction of treatment and presence of protozoa on prokaryotic communities. J. Dairy Sci. 92:3861-3873.
30. Koenig, K. M., C. J. Newbold, F. M. McIntosh, and L. M. Rode. 2000. Effects of protozoa on bacterial nitrogen recycling in the rumen. J. Anim. Sci. 78:2431-2445.
31. Maia, M. R. G., L. C. Chaudhary, L. Figueres, and R. J. Wallace. 2007. Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. Antonie Van Leeuwenhoek 91:303-314.
32. McGuffey, R K, L. F. Richardson, and J. I. D. Wilkinson. 2001. Ionophores for dairy cattle: Current status and future outlook. J. Dairy Sci. 84(E Suppl.):E194-E203.
33. Nagaraja, T. G., C. J. Newbold, C. J. Van Nevel, and D. I. Demeyer. 1997. Manipulation of rumen fermentation. Pages 523-632 in The Rumen Microbial Ecosystem. P. N. Hobson and C. S. Stewart, ed. Chapman and Hall, London, UK.
34. Nagaraja, T. G., and E. C. Titgemeyer. 2007. Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook. J. Dairy Sci. 90(E Suppl.):E17-E38.
35. Oelker, E. R., C. Reveneau, and J. L. Firkins. 2009. Interaction of molasses and monensin in alfalfa hay- or corn silage-based diets on rumen fermentation, total tract digestibility, and milk production by Holstein cows. J. Dairy Sci. 92:270-285.
36. Oldick, B. S., and J. L. Firkins. 2000. Effects of degree of fat saturation on fiber digestion and microbial protein synthesis when diets are fed twelve times daily. J. Anim. Sci. 78:2412-2420.
37. Ozutsumi, Y., K. Tajima, A. Takenaka, and H. Itabashi. 2006. Real- time PCR detection of the effects of protozoa on rumen bacteria in cattle. Curr. Microbiol. 52:158-162.
38. Paillard, D., N. McKain, L. C. Chaudhary, N. D. Walker, F. Pizette, I. Koppova, N. R. McEwan, J. Kopecny, P. E. Vercoe, P. Louis, and R. J. Wallace. 2007. Relation between phylogenetic position, lipid metabolism and butyrate production by different Butyrivibrio-like bacteria from the rumen. Antonie Leeuwenhoek 91:417-422.
39. 18:1. J. Dairy Sci. 87:3473-3479.
40. Ribeiro, C. V. D. M., S. K. R. Karnati, and M. L. Eastridge. 2005. Biohydrogenation of fatty acids and digestibility of fresh alfalfa or alfalfa hay plus sucrose in continuous culture. J. Dairy Sci. 88:4007-4017.
41. Russell, J. B., and A. J. Houlihan. 2003. Ionophore resistance of ruminal bacteria and its potential impact on human health. FEMS Microbiol. Rev. 27:65-74.
42. SAS Institute. 1999. What's New in SAS Software for Version 7 and the Version 8 Developer's Release. Release 7 ed. SAS Inst., Cary, NC.
43. Shingfield, K. J., C. K. Reynolds, G. Hervás, J. M. Griinari, A. S. Grandison, and D. E. Beever. 2006. Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. J. Dairy Sci. 89:714-732.
44. Stewart, C. S., H. J. Flint, and M. P. Bryant. 1997. The rumen bacteria. Pages 10-72 in The Rumen Microbial Ecosystem. P. N. Hobson and C. S. Stewart, ed. Blackie Academic and Professional, New York, NY.
45. Sylvester, J. T., S. K. R. Karnati, B. A. Dehority, M. Morrison, G. L. Smith, N. R. St-Pierre, and J. L. Firkins. 2009. Rumen protozoa decrease generation time and adjust 18S ribosomal DNA copies to adapt to decreased transfer interval, starvation, and monensin. J. Dairy Sci. 92:256-269.
46. Sylvester, J. T., S. K. R. Karnati, Z. Yu, M. Morrison, and J. L. Firkins. 2004. Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR. J. Nutr. 134:3378-3384.
47. Sylvester, J. T., S. K. R. Karnati, Z. Yu, C. J. Newbold, and J. L. Firkins. 2005. Evaluation of a real-time PCR assay for measuring the ruminal pool and duodenal flow of protozoal nitrogen. J. Dairy Sci. 88:2083-2095.
48. Ungerfeld, E. M., S. R. Rust, D. R. Boone, and Y. Liu. 2004. Effects of several inhibitors on pure cultures of ruminal methanogens. J. Appl. Microbiol. 97:520-526.
49. Ushida, K., B. Lassalas, and J.-P. Jouany. 1985. Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry. Influence of sample treatment and preservation. Reprod. Nutr. Dev. 25:1037-1046.
50. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
51. Vlaeminck, B., V. Fievez, D. Demeyer, and R. J. Dewhurst. 2006. Effect of forage:concentrate ratio on fatty acid composition of rumen bacteria isolated from ruminal and duodenal digesta. J. Dairy Sci. 89:2668-2678.
52. Walker, N. D., C. J. Newbold, and R. J. Wallace. 2005. Nitrogen metabolism in the rumen. Pages 71-115 in Nitrogen and Phosphorus Nutrition of Cattle. E. Pfeffer and A. Hristov, ed. CABI Publishing, Cambridge, MA.
53. Weimer, P. J., D. M. Stevenson, D. R. Mertens, and E. E. Thomas. 2008. Effect of monensin feeding and withdrawal on populations of individual bacterial species in the rumen of lactating dairy cows fed high-starch diets. Appl. Microbiol. Biotechnol. 80:135-145.
54. Williams, A. G., and G. S. Coleman. 1992. The Rumen Protozoa. Springer-Verlag, New York, NY.
55. Williams, A. G., and G. S. Coleman. 1997. The rumen protozoa. Pages 73-139 in The Rumen Microbial Ecosystem. P. N. Hobson and C. S. Stewart, ed. Blackie Academic and Professional, New York, NY.
56. Wolin, M. J., T. L. Miller, and C. S. Stewart. 1997. Microbe-microbe interactions. Pages 467-491 in The Rumen Microbial Ecosystem. P. N. Hobson and C. S. Stewart, ed. Blackie Academic and Professional, New York, NY.
57. Wu, Z., O. A. Ohajuruka, and D. L. Palmquist. 1991. Ruminal synthesis, biohydrogenation, and digestibility of fatty acids by dairy cows. J. Dairy Sci. 74:3025-3034.
58. Yáñez-Ruiz, D. R., N. D. Scollan, R. J. Merry, and C. J. Newbold. 2006. Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content. Br. J. Nutr. 96:861-869.
59. Yáñez-Ruiz, D. R., S. Williams, and C. J. Newbold. 2007. The effect of absence of protozoa on rumen biohydrogenation and the fatty acid composition of lamb muscle. Br. J. Nutr. 97:938-948.
60. Ziemer, C. J., R. Sharp, M. D. Stern, M. A. Cotta, T. R. Whitehead, and D. A. Stahl. 2000. Comparison of microbial populations in model and natural rumens using 16S ribosomal RNA-targeted probes. Environ. Microbiol. 2:632-643.
61. Zinn, R. A., and F. N. Owens. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157-166.