메뉴 건너뛰기




Volumn 88, Issue 6, 2005, Pages 2083-2095

Evaluation of a real-time PCR assay quantifying the ruminal pool size and duodenal flow of protozoal nitrogen

Author keywords

Denaturing gradient gel electrophoresis; Real time PCR; rRNA; Rumen protozoal nitrogen

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 26444519840     PISSN: 00220302     EISSN: None     Source Type: Journal    
DOI: 10.3168/jds.S0022-0302(05)72885-X     Document Type: Article
Times cited : (74)

References (53)
  • 1
    • 0036691271 scopus 로고    scopus 로고
    • Supplementing barley or rapeseed meal to dairy cows fed grass-red clover silage. I. Rumen degradability and microbial flow
    • Ahvenjärvi, S., A. Vanhatalo, and P. Huhtanen. 2002. Supplementing barley or rapeseed meal to dairy cows fed grass-red clover silage. I. Rumen degradability and microbial flow. J. Anim. Sci. 80:2176-2187.
    • (2002) J. Anim. Sci. , vol.80 , pp. 2176-2187
    • Ahvenjärvi, S.1    Vanhatalo, A.2    Huhtanen, P.3
  • 3
    • 0025076152 scopus 로고
    • Sequestration, migration and lysis of protozoa in the rumen
    • Ankrah, P., S. C. Loerch, and B. A. Dehority. 1990. Sequestration, migration and lysis of protozoa in the rumen. J. Gen. Microbiol. 136:1869-1875.
    • (1990) J. Gen. Microbiol. , vol.136 , pp. 1869-1875
    • Ankrah, P.1    Loerch, S.C.2    Dehority, B.A.3
  • 5
    • 0025984722 scopus 로고
    • A simple, rapid and reliable method for selecting or assessing the number of replicates for animal experiments
    • Berndtson, W. E. 1991. A simple, rapid and reliable method for selecting or assessing the number of replicates for animal experiments. J. Anim. Sci. 69:67-76.
    • (1991) J. Anim. Sci. , vol.69 , pp. 67-76
    • Berndtson, W.E.1
  • 6
    • 0030679564 scopus 로고    scopus 로고
    • Regulatory sequences for the amplification and replication of the ribosomal DNA minichromosome in Tetrahymena thermophila
    • Blomberg, P., C. Randolph, C.-H. Yao, and M.-C. Yao. 1997. Regulatory sequences for the amplification and replication of the ribosomal DNA minichromosome in Tetrahymena thermophila. Mol. Cell. Biol. 17:7237-7247.
    • (1997) Mol. Cell. Biol. , vol.17 , pp. 7237-7247
    • Blomberg, P.1    Randolph, C.2    Yao, C.-H.3    Yao, M.-C.4
  • 7
    • 0026919883 scopus 로고
    • Markers for quantifying microbial protein synthesis in the rumen
    • Broderick, G. A., and N. R. Merchen. 1992. Markers for quantifying microbial protein synthesis in the rumen. J. Dairy Sci. 75:2618-2632.
    • (1992) J. Dairy Sci. , vol.75 , pp. 2618-2632
    • Broderick, G.A.1    Merchen, N.R.2
  • 8
    • 0035378313 scopus 로고    scopus 로고
    • Site of nutrient digestion by dairy cows fed corn of different particle sizes or steam-rolled
    • Callison, S. L., J. L. Firkins, M. L. Eastridge, and B. L. Hull. 2001. Site of nutrient digestion by dairy cows fed corn of different particle sizes or steam-rolled. J. Dairy Sci. 84:1458-1467.
    • (2001) J. Dairy Sci. , vol.84 , pp. 1458-1467
    • Callison, S.L.1    Firkins, J.L.2    Eastridge, M.L.3    Hull, B.L.4
  • 9
    • 0030156659 scopus 로고    scopus 로고
    • Comparison of nitrogen-15 and purines as microbial markers in continuous culture
    • Calsamiglia, S., M. D. Stern, and J. L. Firkins. 1996. Comparison of nitrogen-15 and purines as microbial markers in continuous culture. J. Anim. Sci. 74:1375-1381.
    • (1996) J. Anim. Sci. , vol.74 , pp. 1375-1381
    • Calsamiglia, S.1    Stern, M.D.2    Firkins, J.L.3
  • 10
    • 0026905680 scopus 로고
    • Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows
    • Clark, J. H., T. H. Klusmeyer, and M. R. Cameron. 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. J. Dairy Sci. 75:2304-2323.
    • (1992) J. Dairy Sci. , vol.75 , pp. 2304-2323
    • Clark, J.H.1    Klusmeyer, T.H.2    Cameron, M.R.3
  • 11
    • 0042712771 scopus 로고
    • The role of rumen protozoa in the metabolism of ruminants given tropical feeds
    • Coleman, G. S. 1979. The role of rumen protozoa in the metabolism of ruminants given tropical feeds. Trop. Anim. Prod. 4:199-212.
    • (1979) Trop. Anim. Prod. , vol.4 , pp. 199-212
    • Coleman, G.S.1
  • 13
    • 0032040251 scopus 로고    scopus 로고
    • Generation times of Epidinium caudatum and Entodinium caudatum, determined in vitro by transferring at various time intervals
    • Dehority, B. A. 1998. Generation times of Epidinium caudatum and Entodinium caudatum, determined in vitro by transferring at various time intervals. J. Anim. Sci. 76:1189-1196.
    • (1998) J. Anim. Sci. , vol.76 , pp. 1189-1196
    • Dehority, B.A.1
  • 14
    • 0942305602 scopus 로고    scopus 로고
    • Nottingham University Press, Nottingham, UK
    • Dehority, B. A. 2003. Rumen Microbiology. Nottingham University Press, Nottingham, UK.
    • (2003) Rumen Microbiology
    • Dehority, B.A.1
  • 15
    • 0031938367 scopus 로고    scopus 로고
    • Quantification of the recycling of microbial nitrogen in the rumen using a mechanistic model of rumen fermentation processes
    • Dijkstra, J., J. France, and S. Tamminga. 1998. Quantification of the recycling of microbial nitrogen in the rumen using a mechanistic model of rumen fermentation processes. J. Agric. Sci. 130:81-94.
    • (1998) J. Agric. Sci. , vol.130 , pp. 81-94
    • Dijkstra, J.1    France, J.2    Tamminga, S.3
  • 16
    • 0029991398 scopus 로고    scopus 로고
    • Maximizing microbial protein synthesis in the rumen
    • Firkins, J. L. 1996. Maximizing microbial protein synthesis in the rumen. J. Nutr. 126:1347S-1354S.
    • (1996) J. Nutr. , vol.126
    • Firkins, J.L.1
  • 17
    • 0032246553 scopus 로고    scopus 로고
    • Modeling ruminal digestibility of carbohydrates and microbial protein flow to the duodenum
    • Firkins, J. L., M. S. Allen, B. S. Oldick, and N. R. St-Pierre. 1998. Modeling ruminal digestibility of carbohydrates and microbial protein flow to the duodenum. J. Dairy Sci. 81:3350-3369.
    • (1998) J. Dairy Sci. , vol.81 , pp. 3350-3369
    • Firkins, J.L.1    Allen, M.S.2    Oldick, B.S.3    St-Pierre, N.R.4
  • 18
    • 0023441890 scopus 로고
    • Ruminal nitrogen metabolism in steers as affected by feed intake and dietary urea concentration
    • Firkins, J. L., L. L. Berger, N. R. Merchen, G. C. Fahey, and R. L. Mulvaney. 1987a. Ruminal nitrogen metabolism in steers as affected by feed intake and dietary urea concentration. J. Dairy Sci. 70:2302-2311.
    • (1987) J. Dairy Sci. , vol.70 , pp. 2302-2311
    • Firkins, J.L.1    Berger, L.L.2    Merchen, N.R.3    Fahey, G.C.4    Mulvaney, R.L.5
  • 19
    • 0023443497 scopus 로고
    • Effects of feed intake and dietary urea concentration on ruminal dilution rate and efficiency of bacterial growth in steers
    • Firkins, J. L., S. M. Lewis, L. Montgomery, L. L. Berger, N. R. Merchen, and G. C. Fahey. 1987b. Effects of feed intake and dietary urea concentration on ruminal dilution rate and efficiency of bacterial growth in steers. J. Dairy Sci. 70:2312-2321.
    • (1987) J. Dairy Sci. , vol.70 , pp. 2312-2321
    • Firkins, J.L.1    Lewis, S.M.2    Montgomery, L.3    Berger, L.L.4    Merchen, N.R.5    Fahey, G.C.6
  • 20
    • 26444502880 scopus 로고    scopus 로고
    • Whole animal nitrogen balance in cattle
    • A. N. Hristov and E. Pfeffer, ed. CAB International, Wallingford, UK
    • Firkins, J. L., and C. K. Reynolds. 2005. Whole animal nitrogen balance in cattle. Pages 167-185 in Nitrogen and Phosphorus Nutrition of Cattle and Environment. A. N. Hristov and E. Pfeffer, ed. CAB International, Wallingford, UK.
    • (2005) Nitrogen and Phosphorus Nutrition of Cattle and Environment , pp. 167-185
    • Firkins, J.L.1    Reynolds, C.K.2
  • 21
    • 0036673330 scopus 로고    scopus 로고
    • Whole linted cottonseed as a forage substitute fed with ground or steam-flaked corn: Digestibility and performance
    • Harvatine, D. I., J. L. Firkins, and M. L. Eastridge. 2002a. Whole linted cottonseed as a forage substitute fed with ground or steam-flaked corn: Digestibility and performance. J. Dairy Sci. 85:1976-1987.
    • (2002) J. Dairy Sci. , vol.85 , pp. 1976-1987
    • Harvatine, D.I.1    Firkins, J.L.2    Eastridge, M.L.3
  • 23
    • 0034152824 scopus 로고    scopus 로고
    • Ruminal fermentation and duodenal flow following progressive inoculations of fauna-free wethers with major individual species of ciliate protozoa or total fauna
    • Ivan, M., L. Neill, and T. Entz. 2000. Ruminal fermentation and duodenal flow following progressive inoculations of fauna-free wethers with major individual species of ciliate protozoa or total fauna. J. Anim. Sci. 78:750-759.
    • (2000) J. Anim. Sci. , vol.78 , pp. 750-759
    • Ivan, M.1    Neill, L.2    Entz, T.3
  • 24
    • 0029926357 scopus 로고    scopus 로고
    • Effect of rumen protozoa on nitrogen utilization by ruminants
    • Jouany, J. P. 1996. Effect of rumen protozoa on nitrogen utilization by ruminants. J. Nutr. 126:13358-13468.
    • (1996) J. Nutr. , vol.126 , pp. 13358-13468
    • Jouany, J.P.1
  • 25
    • 0042882236 scopus 로고    scopus 로고
    • Establishing populations of Megasphaera elsdenii YE34 and Butyrivibrio fibrisolvens YE44 in the rumen of cattle fed high grain diets
    • Klieve, A. V., D. Hennessey, D. Ouwerkerk, R. J. Forster, R. I. Mackie, and G. T. Attwood. 2003. Establishing populations of Megasphaera elsdenii YE34 and Butyrivibrio fibrisolvens YE44 in the rumen of cattle fed high grain diets. J. Appl. Microbiol. 95:621-630.
    • (2003) J. Appl. Microbiol. , vol.95 , pp. 621-630
    • Klieve, A.V.1    Hennessey, D.2    Ouwerkerk, D.3    Forster, R.J.4    Mackie, R.I.5    Attwood, G.T.6
  • 26
    • 0034803204 scopus 로고    scopus 로고
    • Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches
    • Kocherginskaya, S., R. I. Aminov, and B. A. White. 2001. Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches. Anaerobe 7:119-134.
    • (2001) Anaerobe , vol.7 , pp. 119-134
    • Kocherginskaya, S.1    Aminov, R.I.2    White, B.A.3
  • 27
    • 0026162635 scopus 로고
    • Copy number control in Tetrahymena macronuclear genome
    • Larson, D. D., A. R. Umthun, and W.-L. Shaiu. 1991. Copy number control in Tetrahymena macronuclear genome. J. Protozool. 38:258-263.
    • (1991) J. Protozool. , vol.38 , pp. 258-263
    • Larson, D.D.1    Umthun, A.R.2    Shaiu, W.-L.3
  • 28
    • 0021433114 scopus 로고
    • Nitrogen metabolism in the rumen
    • Leng, R. A., and J. V. Nolan. 1984. Nitrogen metabolism in the rumen. J. Dairy Sci. 67:1072-1089.
    • (1984) J. Dairy Sci. , vol.67 , pp. 1072-1089
    • Leng, R.A.1    Nolan, J.V.2
  • 29
    • 10044230138 scopus 로고    scopus 로고
    • Physical effectiveness of whole cottonseed as affected by lint and particle size
    • Abstr.
    • Lima, M. L. M., J. L. Firkins, J. T. Sylvester, S. K. R. Karnati, and W. Mattos. 2003. Physical effectiveness of whole cottonseed as affected by lint and particle size. J. Dairy Sci. 86(Suppl. 1):64. (Abstr.)
    • (2003) J. Dairy Sci. , vol.86 , Issue.1 SUPPL. , pp. 64
    • Lima, M.L.M.1    Firkins, J.L.2    Sylvester, J.T.3    Karnati, S.K.R.4    Mattos, W.5
  • 31
    • 0028541082 scopus 로고
    • Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents
    • Martin, C., A. G. Williams, and B. Michalet-Doreau. 1994. Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. J. Anim. Sci. 72:2962-2968.
    • (1994) J. Anim. Sci. , vol.72 , pp. 2962-2968
    • Martin, C.1    Williams, A.G.2    Michalet-Doreau, B.3
  • 32
    • 23144447463 scopus 로고    scopus 로고
    • A proposed simple method for determining the outflow of ciliates from the reticulo-rumen
    • Michalowski, T. 1998. A proposed simple method for determining the outflow of ciliates from the reticulo-rumen. J. Anim. Feed Sci. 7:83-92.
    • (1998) J. Anim. Feed Sci. , vol.7 , pp. 83-92
    • Michalowski, T.1
  • 34
    • 0030515661 scopus 로고    scopus 로고
    • Comparison of methods for isolation of protozoa from ruminal fluid
    • Neill, L., and M. Ivan. 1996. Comparison of methods for isolation of protozoa from ruminal fluid. Can. J. Anim. Sci. 76:481-483.
    • (1996) Can. J. Anim. Sci. , vol.76 , pp. 481-483
    • Neill, L.1    Ivan, M.2
  • 35
    • 38149147840 scopus 로고
    • Effect of level of intake of kikuyu (Pennisetum clandestinum) grass hay on the flow of protozoal nitrogen to the omasum of cattle
    • Punia, B. S., and J. Leibholz. 1994. Effect of level of intake of kikuyu (Pennisetum clandestinum) grass hay on the flow of protozoal nitrogen to the omasum of cattle. Anim. Feed Sci. Technol. 47:77-87.
    • (1994) Anim. Feed Sci. Technol. , vol.47 , pp. 77-87
    • Punia, B.S.1    Leibholz, J.2
  • 36
    • 0034882664 scopus 로고    scopus 로고
    • Group-specific PCR primers to amplify 24S α-subunit rRNA genes from Kinetoplastida (protozoa) used in denaturing gradient gel electrophoresis
    • Rasmussen, L. D., F. Ekelund, L. H. Hansen, and K. Johnsen. 2001. Group-specific PCR primers to amplify 24S α-subunit rRNA genes from Kinetoplastida (protozoa) used in denaturing gradient gel electrophoresis. Microb. Ecol. 42:109-115.
    • (2001) Microb. Ecol. , vol.42 , pp. 109-115
    • Rasmussen, L.D.1    Ekelund, F.2    Hansen, L.H.3    Johnsen, K.4
  • 39
    • 0034304291 scopus 로고    scopus 로고
    • Partitioning of amino acids flowing to the abomasum into feed, bacterial, protozoal, and endogenous fractions
    • Shabi, Z., H. Tagari, M. R. Murphy, I. Bruckental, S. J. Mabjeesh, S. Zamwell, K. Celik, and A. Arieli. 2000. Partitioning of amino acids flowing to the abomasum into feed, bacterial, protozoal, and endogenous fractions. J. Dairy Sci. 83:2326-2334.
    • (2000) J. Dairy Sci. , vol.83 , pp. 2326-2334
    • Shabi, Z.1    Tagari, H.2    Murphy, M.R.3    Bruckental, I.4    Mabjeesh, S.J.5    Zamwell, S.6    Celik, K.7    Arieli, A.8
  • 40
    • 0032080814 scopus 로고    scopus 로고
    • Taxon-specific associations between protozoal and methanogen populations in the rumen and a model rumen system
    • Sharp, R., C. J. Ziemer, M. D. Stern, and D. A. Stahl. 1998. Taxon-specific associations between protozoal and methanogen populations in the rumen and a model rumen system. FEMS Microbiol. Ecol. 26:71-78.
    • (1998) FEMS Microbiol. Ecol. , vol.26 , pp. 71-78
    • Sharp, R.1    Ziemer, C.J.2    Stern, M.D.3    Stahl, D.A.4
  • 41
    • 6444226211 scopus 로고    scopus 로고
    • Rumen simulation technique study on the interactions of dietary lauric and myristic acid supplementation in suppressing ruminal methanogenesis
    • Soliva, C. R., L. Meile, M. Kreuzer, and A. Machmüller. 2004. Rumen simulation technique study on the interactions of dietary lauric and myristic acid supplementation in suppressing ruminal methanogenesis. Br. J. Nutr. 92:689-700.
    • (2004) Br. J. Nutr. , vol.92 , pp. 689-700
    • Soliva, C.R.1    Meile, L.2    Kreuzer, M.3    Machmüller, A.4
  • 42
    • 0020183901 scopus 로고
    • Estimation of the proportion of non-ammonia-nitrogen reaching the lower gut of the ruminant derived from bacterial and protozoal nitrogen
    • Steinhour, W. D., M. R. Stokes, J. H. Clark, J. A. Rogers, and C. L. Davis. 1982. Estimation of the proportion of non-ammonia-nitrogen reaching the lower gut of the ruminant derived from bacterial and protozoal nitrogen. Br. J. Nutr. 48:417-431.
    • (1982) Br. J. Nutr. , vol.48 , pp. 417-431
    • Steinhour, W.D.1    Stokes, M.R.2    Clark, J.H.3    Rogers, J.A.4    Davis, C.L.5
  • 43
    • 10044284420 scopus 로고    scopus 로고
    • Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR
    • Sylvester, J. T., S. K. R. Karnati, Z. Yu, M. Morrison, and J. L. Firkins. 2004. Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR. J. Nutr. 134:3378-3384.
    • (2004) J. Nutr. , vol.134 , pp. 3378-3384
    • Sylvester, J.T.1    Karnati, S.K.R.2    Yu, Z.3    Morrison, M.4    Firkins, J.L.5
  • 44
    • 0025198344 scopus 로고
    • Omasal ciliated protozoa in cattle, bison, and sheep
    • Towne, G., and T. G. Nagaraja. 1990. Omasal ciliated protozoa in cattle, bison, and sheep. Appl. Environ. Microbiol. 56:409-412.
    • (1990) Appl. Environ. Microbiol. , vol.56 , pp. 409-412
    • Towne, G.1    Nagaraja, T.G.2
  • 45
    • 0019040854 scopus 로고
    • Investigation of chromium, cerium and cobalt as markers in digesta rate of passage studies
    • Uden, P., P. E. Colucci, and P. J. Van Soest. 1980. Investigation of chromium, cerium and cobalt as markers in digesta rate of passage studies. J. Sci. Food Agric. 31:625-632.
    • (1980) J. Sci. Food Agric. , vol.31 , pp. 625-632
    • Uden, P.1    Colucci, P.E.2    Van Soest, P.J.3
  • 46
    • 0022389062 scopus 로고
    • Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry. Influence of sample treatment and preservation
    • Ushida, K., B. Lassalas, and J. P. Jouany. 1985. Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry. Influence of sample treatment and preservation. Reprod. Nutr. Dev. 25:1037-1046.
    • (1985) Reprod. Nutr. Dev. , vol.25 , pp. 1037-1046
    • Ushida, K.1    Lassalas, B.2    Jouany, J.P.3
  • 47
    • 0033474368 scopus 로고    scopus 로고
    • Chemical composition of protozoal and bacterial fractions isolated from ruminal contents of dairy cows fed diets differing in nitrogen supplementation
    • Volden, H., L. T. Mydland, and O. M. Harstad. 1999. Chemical composition of protozoal and bacterial fractions isolated from ruminal contents of dairy cows fed diets differing in nitrogen supplementation. Acta Agric. Scand. A Anim. Sci. 49:235-244.
    • (1999) Acta Agric. Scand. A Anim. Sci. , vol.49 , pp. 235-244
    • Volden, H.1    Mydland, L.T.2    Harstad, O.M.3
  • 49
    • 0027338468 scopus 로고
    • Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep
    • Williams, A. G., and S. E. Withers. 1993. Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep. Can. J. Microbiol. 39:61-69.
    • (1993) Can. J. Microbiol. , vol.39 , pp. 61-69
    • Williams, A.G.1    Withers, S.E.2
  • 50
    • 1542397626 scopus 로고
    • The determination of chromic oxide in feces samples by atomic absorption spectrophotometry
    • Williams, C. H., D. J. David, and O. Iismaa. 1962. The determination of chromic oxide in feces samples by atomic absorption spectrophotometry. J. Agric. Sci. 59:381-385.
    • (1962) J. Agric. Sci. , vol.59 , pp. 381-385
    • Williams, C.H.1    David, D.J.2    Iismaa, O.3
  • 51
    • 0029150755 scopus 로고
    • Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes
    • Yu, W., W. K. Dodds, M. K. Banks, J. Skalsky, and E. A. Strauss. 1995. Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes. Appl. Environ. Microbiol. 61:3367-3372.
    • (1995) Appl. Environ. Microbiol. , vol.61 , pp. 3367-3372
    • Yu, W.1    Dodds, W.K.2    Banks, M.K.3    Skalsky, J.4    Strauss, E.A.5
  • 52
    • 0001322472 scopus 로고
    • A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis
    • Zinn, R. A., and F. N. Owens. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157-166.
    • (1986) Can. J. Anim. Sci. , vol.66 , pp. 157-166
    • Zinn, R.A.1    Owens, F.N.2
  • 53
    • 0942279586 scopus 로고    scopus 로고
    • Molecular ecological analysis of the gastrointestinal microbiota: A review
    • Zoetendal, E. G., C. T. Collier, S. Koike, R. I. Mackie, and H. R. Gaskins. 2004. Molecular ecological analysis of the gastrointestinal microbiota: A review. J. Nutr. 134:465-472.
    • (2004) J. Nutr. , vol.134 , pp. 465-472
    • Zoetendal, E.G.1    Collier, C.T.2    Koike, S.3    Mackie, R.I.4    Gaskins, H.R.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.