Rumen ciliate protozoa contain high concentrations of conjugated linoleic acids and vaccenic acid, yet do not hydrogenate linoleic acid or desaturate stearic acid

British Journal of Nutrition

Volumn 96, Issue 4, 2006, Pages 697-704

  Devillard, Estelle ^a   McIntosh, Freda M ^a   Newbold, C James ^b   Wallace, R John ^a  

^a ROWETT RESEARCH INSTITUTE   (United Kingdom)

^b ABERYSTWYTH UNIVERSITY   (United Kingdom)

Author keywords

Biohydrogenation; Conjugated linoleic acids; Rumen protozoa; Trans fatty acids

Indexed keywords

ACYL COENZYME A DESATURASE; COMPLEMENTARY DNA; CONJUGATED LINOLEIC ACID; LINOLEIC ACID; STEARIC ACID; UNSATURATED FATTY ACID; VACCENIC ACID; 11-OCTADECENOIC ACID; OLEIC ACID; STEARIC ACID DERIVATIVE;

ANIMAL TISSUE; ARTICLE; CILIATE; CONTROLLED STUDY; DNA LIBRARY; FATTY ACID DESATURATION; FATTY ACID METABOLISM; INCUBATION TIME; LIPID COMPOSITION; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTOZOON; RUMEN MICROORGANISM; RUMINANT STOMACH; SHEEP; SPECIES DIFFERENCE; ANIMAL; BACTERIUM; BIOSYNTHESIS; CHEMISTRY; HYDROGENATION; METABOLISM; PARASITOLOGY; PHYSIOLOGY; STOMACH JUICE;

ANIMALIA; BACTERIA (MICROORGANISMS); CILIOPHORA; ENTODINIUM; EPIDINIUM ECAUDATUM; OVIS ARIES; PROTOZOA;

ANIMALS; BACTERIA; GASTROINTESTINAL CONTENTS; HYDROGENATION; LINOLEIC ACID; LINOLEIC ACIDS, CONJUGATED; MALE; OLEIC ACIDS; PROTOZOA; RUMEN; SHEEP; STEARIC ACIDS;

EID: 33947112073     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1079/BJN20061884     Document Type: Article

References (40)

1. Abaza MA, Abou Akkada AR & El Shazly K (1975) Effect of rumen protozoa on dietary lipid in sheep. J Agric Sci 85, 135-143.
2. Abe M, Iriki T, Tobe N & Shibui H (1981) Sequestration of holotrich protozoa in the reticulo-rumen of cattle. Appl Environ Microbiol 41, 758-765.
3. Ankrah P, Loerch SC & Dehority BA (1990) Sequestration, migration and lysis of protozoa in the rumen. J Gen Microbiol 136, 1869-1875.
4. Belury MA (2002) Dietary conjugated linoleic acid in health: physiological effects and mechanisms of action. Annu Rev Nutr 22, 505-531.
5. 14C-acid by rumen protozoa. J Anim Sci 27, 1502-1508.
6. Coleman GS (1992) The rate of uptake and metabolism of starch grains and cellulose particles by Entodinium species, Eudiplodinium maggii, some other entodiniomorphid protozoa and natural protozoal populations taken from the ovine rumen. J Appl Bacteriol 73, 507-513.
7. Dawson RM & Kemp P (1969) The effect of defaunation on the phospholipids and on the hydrogenation of unsaturated fatty acids in the rumen. Biochem J 115, 351-352.
8. Emmanuel B (1974) On the origin of rumen protozoan fatty acids. Biochim Biophys Acta 337, 404-413.
9. Folch J, Lees M & Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509.
10. Girard V & Hawke JC (1978) The role of holotrichs in the metabolism of dietary linoleic acid in the rumen. Biochim Biophys Acta 528, 17-27.
11. Griinari JM, Corl BA, Lacy SH, Chouinard PY, Nurmela KV & Bauman DE (2000) Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta(9)-desaturase. J Nutr 130, 2285-2291.
12. Harfoot CG (1978) Lipid metabolism in the rumen. Progr Lipid Res 17, 21-54.
13. Harfoot CG & Hazlewood GP (1997) Lipid metabolism in the rumen. In The Rumen Microbial Ecosystem, 2nd ed. pp. 382-426 [PN Hobson and CS Stewart, editors]. London: Chapman & Hall.
14. Herbert D, Phipps PJ & Strange RE (1971) Chemical analysis of microbial cells. In Methods in Microbiology, vol. 5B, pp. 209-344 [JR Norris and DW Ribbons, editors]. London: Academic Press.
15. Hungate RE, Reichl J & Prins R (1971) Parameters of fermentation in a continuously fed sheep: Evidence of a microbial rumination pool. Appl Microbiol 22, 1104-1113.
16. Jouany JP & Lassalas B (2003) Effects of rumen protozoa on biohydrogenation of polyunsaturated fatty acids and synthesis of conjugated linoleic acids. In Proceedings of the International Conference on the Molecular Biology and Biotechnology of Ciliates and Anaerobic Protozoa. Nijmegen. The Netherlands: German Society for Protozoology.
17. Jouany JP & Senaud J (1979) Défaunation du rumen de mouton. Ann Biol Anim Biochem Biophys 19, 619-624.
18. Kaluzny MA, Duncan LA, Merritt MV & Epps DE (1985) Rapid separation of lipid classes in high yield and purity using bonded phase column. J Lipid Res 26, 135-140.
19. Katz I & Keeney M (1966) Characterization of the octadecenoic acids in rumen digesta and rumen bacteria. J Dairy Sci 49, 962-966.
20. Keeney M (1970) Lipid metabolism in the rumen. In Physiology and Metabolism in the Ruminant, pp. 489-503 [AT Phillipson, editor]. Newcastle-upon-Tyne: Oriel Press.
21. Klopfenstein TJ, Purser DB & Tyznik WJ (1966) Effect of defaunation on feed digestibility, rumen metabolism and blood metabolites. J Anim Sci 25, 765-773.
22. Los DA & Murata N (1998) Structure and expression of fatty acid desaturases. Biochim Biophys Acta 1394, 3-15.
23. Martin C, Devillard E & Michalet-Doreau B (1999) Influence of sampling site on concentrations and carbohydrate-degrading enzyme activities of protozoa and bacteria in the rumen. J Anim Sci 77, 979-987.
24. Noble RC (1981) Digestion, absorption and transport of lipids in ruminant animals. In Lipid Metabolism in Ruminant Animals, pp. 57-93 [WW Christie, editor]. Oxford: Pergamon Press.
25. Pariza MW (2004) Perspective on the safety and effectiveness of conjugated linoleic acid. Am J Clin Nutr 79, 1132S-1136S.
26. Pereira SL, Leonard AE & Mukerji P (2003) Recent advances in the study of fatty acid desaturases from animals and lower eukaryotes. Prostaglandins Leukot Essent Fatty Acids 68, 97-106.
27. Polan CE, McNeill JJ & Tove SB (1964) Biohydrogenation of unsaturated fatty acids. J Bact 88, 1056-1064.
28. Ricard G, McEwan NR, Dutilh BE, et al. (2006) Horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic carbohydrates rich environment. BMC Genomics 7, 22.
29. Ritzenthaler KL, McGuire MK, Falen R, Shultz TD, Dasgupta N & McGuire MA (2001) Estimation of conjugated linoleic acid intake by written dietary assessment methodologies underestimates actual intake evaluated by food duplicate methodology. J Nutr 131, 1548-1554.
30. Singh S & Hawke JC (1979) The in vitro lipolysis and biohydrogenation of monogalactosyldiglyceride by whole rumen contents and its fractions. J Sci Food Agric 30, 603-612.
31. Tonon T, Harvey D, Qing R, Li Y, Larson TR & Graha IA (2004) Identification of a fatty acid Delta 11-desaturase from the microalga Thalassiosira pseudonana. FEBS Lett 563, 28-34.
32. van de Vossenberg JL & Joblin KN (2003) Biohydrogenation of C18 unsaturated fatty acids to stearic acid by a strain of Butyrivibrio hungatei from the bovine rumen. Lett Appl Microbiol 37, 424-428.
33. van Hoek AH, van Alen TA, Sprakel VS, Hackstein JH & Vogels GD (1998) Evolution of anaerobic ciliates from the gastrointestinal tract: phylogenetic analysis of the ribosomal repeat from Nyctotherus ovalis and its relatives. Mol Biol Evol 15, 1195-1206.
34. Viviani R (1970) Metablism of long-chain fatty acids in the rumen. Adv Lipid Res 8, 267-346.
35. Wasowska I, Maia MRG, Niedzwiedzka KM, Czauderna M, Ramalho Ribeiro JMC, Devillard E, Shingfield KJ & Wallace RJ (2006) Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. Br J Nutr 95, 1199-1211.
36. Weller RA & Pilgrim AF (1974) Passage of protozoa and volatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system. Br J Nutr 32, 341-351.
37. Williams AG & Coleman GS (1992) The Rumen Protozoa. New York: Springer-Verlag.
38. Wongwathanarat P, Michaelson LV, Carter AT, Lazarus CM, Griffiths G, Stobart AK, Archer DB & MacKenzie DA (1999) Two fatty acid delta9- desaturase genes, ole1 and ole2, from Mortierella alpina complement the yeast ole1 mutation, Microbiology 145, 2939-2946.
39. Wright DE (1959) Hydrogenation of lipids by rumen protozoa. Nature 184, 875-876.
40. Wright DE (1960) Hydrogenation of chloroplast lipids by rumen bacteria. Nature 185, 546-547.