Red fluorescent protein pH biosensor to detect concentrative nucleoside transport

Journal of Biological Chemistry

Volumn 284, Issue 31, 2009, Pages 20499-20511

  Johnson, Danielle E ^a   Ai, Hui Wang ^a,b   Wong, Peter ^a   Young, James D ^a   Campbell, Robert E ^a   Casey, Joseph R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID ENVIRONMENT; CHIMERIC PROTEINS; CONCENTRATIVE NUCLEOSIDE TRANSPORTERS; COUPLED TRANSPORT; CYTOSOLIC; HEK293 CELLS; MAMMALIAN CELLS; NEGATIVE CONTROL; NEW APPROACHES; NUCLEOSIDE TRANSPORT; PH SENSITIVE; RED FLUORESCENT PROTEINS; SELF-REFERENCING; TRANSPORT ACTIVITY; TRANSPORT RATE;

BIOSENSORS; CELL MEMBRANES; FLUORESCENCE; MAMMALS; POTENTIOMETRIC SENSORS; SODIUM;

BIOMOLECULES;

2,7 BIS(CARBOXYETHYL) 6 CARBOXYFLUORESCEIN; HUMAN CONCENTRATIVE NUCLEOSIDE TRANSPORTER 3; MNECTARINE PROTEIN; NUCLEOSIDE TRANSPORTER; PROTON; RED FLUORESCENT PROTEIN; SODIUM ION; UNCLASSIFIED DRUG; URIDINE;

ACIDIFICATION; AMINO TERMINAL SEQUENCE; ARTICLE; BIOSENSOR; CELL STRAIN HEK293; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; NUCLEOSIDE TRANSPORT; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN VARIANT;

BIOLOGICAL TRANSPORT; BIOSENSING TECHNIQUES; CELL LINE; CYTOSOL; FLUORESCEINS; GENETIC ENGINEERING; GLYCOSYLATION; HUMANS; HYDROGEN-ION CONCENTRATION; INTRACELLULAR SPACE; KINETICS; LUMINESCENT PROTEINS; MUTANT PROTEINS; NUCLEOSIDES; PHOTOBLEACHING; PROTONS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; URIDINE;

MAMMALIA;

EID: 68949122860     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.019042     Document Type: Article

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