The role of apoptotic cell death in the radiosensitising effect of gemcitabine

British Journal of Cancer

Volumn 101, Issue 4, 2009, Pages 628-636

  Pauwels, B ^a   Vermorken, J B ^a   Wouters, A ^a   Ides, J ^a   Van Laere, S ^a   Lambrechts, H A J ^a   Pattyn, G G O ^a   Vermeulen, K ^a   Meijnders, P ^b   Lardon, F ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

^b University Radiotherapy Antwerp URA   (Belgium)

Author keywords

Apoptosis; Cell cycle; Gemcitabine; Radiation; Radiosensitisation

Indexed keywords

CASPASE 8; CASPASE 9; GEMCITABINE; PROTEIN BID; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND;

APOPTOSIS; ARTICLE; CANCER RADIOTHERAPY; CELL CYCLE ARREST; CELL CYCLE S PHASE; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVATION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; RADIOSENSITIZATION;

APOPTOSIS; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; BLOTTING, WESTERN; CASPASE 8; CASPASE 9; CELL LINE, TUMOR; DEOXYCYTIDINE; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMANS; IN SITU NICK-END LABELING; MEMBRANE POTENTIAL, MITOCHONDRIAL; POLYMERASE CHAIN REACTION; RADIATION-SENSITIZING AGENTS;

EID: 68749115264     PISSN: 00070920     EISSN: 15321827     Source Type: Journal    
DOI: 10.1038/sj.bjc.6605145     Document Type: Article

References (47)

1. Abend M (2003) Reasons to reconsider the significance of apoptosis for cancer therapy. Int J Radiat Biol 79: 927-941
2. Brown JM, Wilson G (2003) Apoptosis genes and resistance to cancer therapy: what does the experimental and clinical data tell us? Cancer Biol Ther 2: 477-490
3. Brown JM, Wouters BG (2001) Apoptosis: mediator or mode of cell killing by anticancer agents? Drug Resist Updat 4: 135-136
4. Chang G, Hsu S, Tsai J, Wu W, Chen C, Sheu G (2004) Extracellular signalregulated kinase activation and Bcl-2 downregulation mediate apoptosis after gemcitabine treatment partly via a p53-independent pathway. Eur J Pharmacol 502: 169-183
5. Christgen M, Schniewind B, Jueschke A, Ungefroren H, Kalthoff H (2005) Gemcitabine-mediated apoptosis is associated with increased CD95 surface expression but is not inhibited by DN-FADD in Colo357 pancreatic cancer cells. Cancer Lett 227: 193-200
6. Creane M, Seymour CB, Colucci S, Mothersill C (1999) Radiobiological effects of docetaxel (Taxotere): a potential radiation sensitizer. Int J Radiat Biol 75: 731-737
7. Debatin KM, Krammer PH (2004) Death receptors in chemotherapy and cancer. Oncogene 23: 2950-2966
8. Doyle TH, Mornex F, McKenna G (2001) The clinical implications of gemcitabine radiosensitization. Clin Cancer Res 7: 226-228
9. Ferreira CG, Span SW, Peters GJ, Kruyt FAE, Giaccone G (2000a) Chemotherapy triggers apoptosis in a caspase-8-dependent and mitochondria-controlled manner in the non-small cell lung cancer cell line NCI-H460. Cancer Res 60: 7133-7141
10. Ferreira CG, Tolis C, Span SW, Peters GJ, van Lopik T, Kummer AJ, Pinedo HM, Giaccone G (2000b) Drug-induced apoptosis in lung cancer cells is not mediated by the Fas/FasL (CD95/APO1) signaling pathway. Clin Cancer Res 6: 203-212
11. Fukuoka K, Arioka H, Iwamoto Y, Fukumoto H, Kurokawa H, Ishida T, Tomonari A, Suzuki T, Usuda J, Kanzawa F, Saijo N, Nishio K (2001) Mechanism of the radiosensitization induced by vinorelbine in human non-small cell lung cancer cells. Lung Cancer 34: 451-460
12. Fulda S, Debatin KM (2006) Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene 25: 4798-4811
13. Gazzaniga P, Silvestri I, Gradilone A, Scarpa S, Morrone S, Gandini O, Gianni W, Frati L, Agliano AM (2007) Gemcitabine-induced apoptosis in 5637 cell line: an in-vitro model for high-risk superficial bladder cancer. Anticancer Drugs 18: 179-185
14. Goeman JJ, van de Geer SA, de KF, van Houwelingen HC (2004) A global test for groups of genes: testing association with a clinical outcome. Bioinformatics 20: 93-99
15. Green D (1998) Apoptotic pathways: the roads to ruin. Cell 94: 695-698
16. Green D (2000) Apoptotic pathways: paper wraps stone blunts scissors. Cell 102: 1-4
17. Habiro A, Tanno S, Koizumi K, Izawa T, Nakano Y, Osanai M, Mizukami Y, Okumura T, Kohgo Y (2004) Involvement of p38 mitogen-activated protein kinase in gemcitabine-induced apoptosis in human pancreatic cancer cells. Biochem Biophys Res Commun 316: 71-77
18. Hermann RM, Rave-Frank M, Pradier O (2008) Combining radiation with oxaliplatin: a review of experimental results. Cancer Radiother 12: 61-67
19. Huang P, Ballal K, Plunkett W (1997) Biochemical characterization of the protein activity responsible for high molecular weight DNA fragmentation during drug-induced apoptosis. Cancer Res 57: 3407-3414
20. Huang P, Plunkett W (1995a) Fludarabine- and gemcitabine-induced apoptosis: incorporation of analogs into DNA is a critical event. Cancer Chemother Pharmacol 36: 181-188
21. Huang P, Plunkett W (1995b) Induction of apoptosis by gemcitabine. Semin Oncol 22: 19-25
22. Kurdow R, Schniewind B, Zoefelt S, Boenicke L, Boehle A, Dohrmann P, Kalthoff H (2005) Apoptosis by gemcitabine in non-small cell lung cancer cell line KNS62 is induced downstream of caspase 8 and is profoundly blocked by Bcl-xL over-expression. Langenbecks Arch Surg 390: 243-248
23. Kuribayashi K, El-Deiry WS (2008) Regulation of programmed cell death by the p53 pathway. Adv Exp Med Biol 615: 201-221
24. Lawrence TS, Davis MA, Hough A, Rehemtulla A (2001) The role of apoptosis in 2′,2′-difluoro-2′-deoxycytidine (gemcitabine)-mediated radiosensitization. Clin Cancer Res 7: 314-319
25. Liu XM, Wang LG, Kreis W, Budman DR, Adams LM (2001) Differential effect of vinorelbine versus paclitaxel on ERK2 kinase activity during apoptosis in MCF-7 cells. Br J Cancer 85: 1403-1411
26. Mose S, Class R, Weber HW, Rahn A, Brady LW, Bottcher HD (2003) Radiation enhancement by gemcitabine-mediated cell cycle modulations. Am J Clin Oncol 26: 60-69
27. Nabhan C, Gajria D, Krett NL, Gandhi V, Ghias K, Rosen ST (2002) Caspase activation is required for gemcitabine activity in multiple myeloma cell lines. Mol Cancer Ther 1: 1221-1227
28. Okamoto K, Ocker M, Neureiter D, Dietze O, Zopf S, Hahn EG, Herold C (2007) bcl-2-specific siRNAs restore gemcitabine sensitivity in human pancreatic cancer cells. J Cell Mol Med 11: 349-361
29. Ostruszka LJ, Shewach DS (2000) The role of cell cycle progression in radiosensitization by 2′,2′-difluoro-2′-deoxycytidine. Cancer Res 60: 6080-6088
30. Pace E, Melis M, Siena L, Bucchieri F, Vignola AM, Profita M, Gjomarkaj M, Bonsignore G (2000) Effects of gemcitabine on cell proliferation and apoptosis in non-small-cell lung cancer (NSCLC) cell lines. Cancer Chemother Pharmacol 46: 467-476
31. Pauwels B, Korst A, Lardon F, Vermorken J (2005a) Combined modality therapy of gemcitabine and radiation. Oncologist 10: 34-51
32. Pauwels B, Korst AEC, Andriessen V, Baay MFD, Pattyn GGO, Lambrechts HAJ, De Pooter CMJ, Lardon F, Vermorken JB (2005b) Unravelling the mechanism of radiosensitisation by gemcitabine: the role of p53. Radiat Res 164: 642-650
33. Pauwels B, Korst AEC, De Pooter CMJ, Lambrechts HAJ, Pattyn GGO, Lardon F, Vermorken JB (2003a) The radiosensitising effect of gemcitabine and the influence of the rescue agent amifostine in vitro. Eur J Cancer 39: 838-846
34. Pauwels B, Korst AEC, De Pooter CMJ, Pattyn GGO, Lambrechts HAJ, Baay MFD, Lardon F, Vermorken JB (2003b) Comparison of the sulforhoda-mine B assay and the clonogenic assay for in vitro chemoradiation studies. Cancer Chemother Pharmacol 51: 221-226
35. Pauwels B, Korst AEC, Pattyn GGO, Lambrechts HAJ, Van Bockstaele DR, Vermeulen K, Lenjou M, De Pooter CMJ, Vermorken JB, Lardon F (2003c) Cell cycle effect of gemcitabine and its role in the radiosensitizing mechanism in vitro. Int J Radiat Oncol Biol Phys 57: 1075-1083
36. Rich TA (2003) Camptothecin radiation sensitization. In Chemoradiation in Cancer Therapy Choy H (ed), pp 93-105. Humana Press: Totowa
37. Schniewind B, Christgen M, Kurdow R, Haye S, Kremer B, Kalthoff H, Ungefroren H (2004) Resistance of pancreatic cancer to gemcitabine treatment is dependent on mitochondria-mediated apoptosis. Int J Cancer 109: 182-188
38. Seol JW, Chaudhari AA, Lee YJ, Kang HS, Kim IS, Kim NS, Park JH, Kim TH, Seol DW, Park SY (2007) Regulation of DR-5 protein and mitochondrial transmembrane potential by gemcitabine, a possible mechanism of gemcitabine-enhanced TRAIL-induced apoptosis. Oncol Rep 18: 523-529
39. Shewach DS, Lawrence TS (1996) Radiosensitization of human solid tumor cell lines with gemcitabine. Semin Oncol 23: 65-71
40. Shewach DS, Lawrence TS (2007) Antimetabolite radiosensitizers. J Clin Oncol 25: 4043-4050
41. Sinclair WK (1972) Sensitivity to mitotic delay and stage in the cycle. Curr Top Radiat Res 7: 323-327
42. Tang HY, Davis MA, Strickfaden SM, Maybaum J, Lawrence TS (1997) Influence of cell cycle phase on radiation-induced toxicity and DNA damage in human colon cancer (HT-29) and chinese hamster ovary cells. Radiat Res 138: S109-S112
43. Tannock IF, Lee C (2001) Evidence against apoptosis as a major mechanism for reproductive cell death following treatment of cell lines with anticancer drugs. Br J Cancer 84: 100-105
44. Thorburn A (2007) Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway signaling. J Thorac Oncol 2: 461-465
45. Tolis C, Peters GJ, Ferreira CG, Pinedo HM, Giaccone G (1999) Cell cycle disturbances and apoptosis induced by topotecan and gemcitabine on human lung cancer cell lines. Eur J Cancer 35: 796-807
46. Wachters FM, van Putten JWG, Maring JG, Zdzienicka MZ, Groen HJM, Kampinga HH (2003) Selective targeting of homologous DNA recombination repair by gemcitabine. Int J Radiat Oncol Biol Phys 57: 553-562
47. Zhang M, Boyer M, Rivory L, Hong A, Clarke S, Stevens G, Fife K (2004) Radiosensitization of vinorelbine and gemcitabine in NCI-H460 non-small-cell lung cancer cells. Int J Radiat Oncol Biol Phys 58: 353-360