Development of an enzyme activity screening system for β-glucosidase- displaying yeasts using calcium alginate micro-beads and flow sorting

Applied Microbiology and Biotechnology

Volumn 84, Issue 2, 2009, Pages 375-382

  Zhou, Yan ^a   Kajiyama, Shin'Ichiro ^b   Itoh, Kouichi ^a   Tanino, Takanori ^c,d   Fukuda, Nobuo ^c   Tanaka, Tsutomu ^c   Kondo, Akihiko ^c   Fukui, Kiichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b KINKI UNIVERSITY   (Japan)

^c KOBE UNIVERSITY   (Japan)

^d GUNMA UNIVERSITY   (Japan)

Author keywords

B glucosidase; Calciumalginate beads; Screening system; Yeast cell surface display

Indexed keywords

B-GLUCOSIDASE; CALCIUM ALGINATE; CALCIUMALGINATE BEADS; ENZYMATIC REACTION; FLUORESCENT MOLECULES; GLUCOSIDASE; GLYCOSIDASES; HIGH-SPEED; MICROBEADS; MIXED SUSPENSIONS; NEGATIVE CONTROL; POTENTIAL APPLICATIONS; REVERSE-MICELLE METHOD; SCREENING SYSTEM; YEAST CELL; YEAST CELL SURFACE DISPLAY;

CALCIUM; CALCIUM ALLOYS; CATALYSTS; ENZYME ACTIVITY; ENZYMES; FLOW CYTOMETRY; FLUORESCENCE; SUBSTRATES; SUSPENSIONS (FLUIDS); YEAST;

CELL MEMBRANES;

BETA GLUCOSIDASE; CALCIUM ALGINATE; HYDROLASE;

DIFFUSION; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE; HYDROLYSIS; REACTION KINETICS; RESEARCH WORK; SUBSTRATE; YEAST;

ARTICLE; CELL SCREENING; CONTROLLED STUDY; DIFFUSION; ENZYME ACTIVITY; FLOW CYTOMETRY; HYDROLYSIS; NONHUMAN; REVERSE MICELLE; SACCHAROMYCES CEREVISIAE; SUSPENSION; YEAST CELL;

ALGINATES; BETA-GLUCOSIDASE; CELL SEPARATION; FLOW CYTOMETRY; GLUCURONIC ACID; HEXURONIC ACIDS; MICROSPHERES; OCTANES; YEASTS;

EID: 68749106826     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2091-8     Document Type: Article

References (18)

1. ET Boder KD Wittrup 2007 Yeast surface display for screening combinatorial polypeptide libraries Nat Biotechnol 15 553 557 10.1038/nbt0697-553
2. G Ciofani V Raffa A Menciassi S Micera P Dario 2007 A drug delivery system based on alginate microspheres: mass-transport test and in vitro validation Biomed Microdevices 9 395 403 10.1007/s10544-006-9044-0 (Pubitemid 46887785)
3. P Forrer S Jung A Plückthun 1999 Beyond binding: using phage display to select for structure, folding and enzymatic activity in proteins Curr Opin Struct Biol 9 4 514 520 10.1016/S0959-440X(99)80073-6 (Pubitemid 29377897)
4. G Georgiou HL Poetschke C Stathopoulos JA Francisco 1993 Practical applications of engineering gram-negative bacterial cell surfaces Trends Biotechnol 11 6 10 10.1016/0167-7799(93)90068-K
5. G Georgiou C Stathopoulos PS Daugherty AR Nayak BL Iverson R Curtiss 3rd 1997 Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines Nat Biotechnol 15 29 34 10.1038/nbt0197-29
6. A Kondo M Ueda 2004 Yeast cell-surface display-applications of molecular display Appl Microbiol Biotechnol 64 28 40 10.1007/s00253-003-1492-3 (Pubitemid 38392150)
7. YJ Kwon CA Peng 2002 Calcium-alginate gel bead cross-linked with gelatin as microcarrier for anchorage-dependent cell culture Biotechniques 33 212 214 216, 218
8. H Liu A Kawabe S Matsunaga T Murakawa A Mizukami M Yanagisawa E Nagamori S Harashima A Kobayashi K Fukui 2004 Obtaining transgenic plants using the bio-active beads method J Plant Res 117 95 99 10.1007/s10265-003-0141-3 (Pubitemid 38642030)
9. A Mizukami E Nagamori Y Takakura S Matsunaga Y Kaneko S Kajiyama S Harashima A Kobayashi K Fukui 2003 Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA Biotechniques 35 734-736 738 740
10. T Murai M Ueda T Kawaguchi M Arai A Tanaka 1998 Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing beta-glucosidase and carboxymethylcellulase from Aspergillus aculeatus Appl Environ Microbiol 64 4857 4861
11. MJ Olsen D Stephens D Griffiths P Daugherty G Georgiou BL Iverson 2000 Function-based isolation of novel enzymes from a large library Nat Biotechnol 18 1071 1074 10. 1038/80267
12. GP Smith 1985 Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface Sciencen 228 1315 1317
13. T Sone E Nagamori T Ikeuchi A Mizukami Y Takakura S Kajiyama E Fukusaki S Harashima A Kobayashi K Fukui 2002 A novel gene delivery system in plants with calcium alginate micro-beads J Biosci Bioeng 94 87 91 10.1016/S1389-1723(02) 80123-2
14. S Ståhl M Uhlén 1997 Bacterial surface display: trends and progress Trends Biotechnol 15 185 192 10.1016/S0167-7799(97)01034-2 (Pubitemid 27219577)
15. MM Stevens HF Qanadilo R Langer V Prasad Shastri 2004 A rapid-curing alginate gel system: utility in periosteum-derived cartilage tissue engineering Biomaterials 25 887 894 10.1016/j.biomaterials.2003.07.002 (Pubitemid 37371996)
16. M Ueda A Tanaka 2000 Cell surface engineering of yeast: construction of arming yeast with biocatalyst J Biosci Bioeng 90 125 136 10.1016/S1389-1723(00) 80099-7 (Pubitemid 30749471)
17. Y Urano M Kamiya K Kanda T Ueno K Hirose T Nagano 2005 Evolution of fluorescein as a platform for finely tunable fluorescence probes J Am Chem Soc 127 4888 4894 10.1021/ja043919h (Pubitemid 40489642)
18. L Wang RM Shelton PR Cooper M Lawson JT Triffitt JE Barralet 2003 Evaluation of sodium alginate for bone marrow cell tissue engineering Biomaterials 24 3475 3481 10.1016/S0142-9612(03)00167-4 (Pubitemid 36703122)