Antigen retrieval techniques in immunohistochemistry: Comparison of different methods

Journal of Pathology

Volumn 183, Issue 1, 1997, Pages 116-123

  Pileri, Stefano A ^a,d   Roncador, Giovanna ^a   Ceccarelli, Claudio ^a   Piccioli, Milena ^a   Briskomatis, Aspasia ^a   Sabattini, Elena ^a   Ascani, Stefano ^a   Santini, Donatella ^a   Piccaluga, Pier Paolo ^a   Leone, Ornella ^a   Damiani, Stefania ^a   Ercolessi, Cesarina ^a   Sandri, Federica ^a   Pieri, Federica ^a   Leoncini, Lorenzo ^b   Falini, Brunangelo ^c  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF SIENA   (Italy)

^c UNIVERSITY OF PERUGIA   (Italy)

^d UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Antigen retrieval; Fixation; Immunohistochemistry; Standardization

Indexed keywords

ALKALINE PHOSPHATASE; EDETIC ACID;

ANTIGEN PURIFICATION; ARTICLE; IMMUNOHISTOCHEMISTRY; IMMUNOPEROXIDASE STAINING; PRIORITY JOURNAL; STAINING; TISSUE FIXATION; TISSUE PREPARATION;

ANTIGENS; ANTIGENS, NEOPLASM; BONE MARROW; EDETIC ACID; HEAT; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; IMMUNOENZYME TECHNIQUES; MICROWAVES; PEPTIDE HYDROLASES; PRESSURE; TISSUE FIXATION;

EID: 0030846045     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199709)183:1<116::AID-PATH1087>3.0.CO;2-2     Document Type: Article

References (29)

1. Pileri S, Serra L, Martinelli G. The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins. Bas Appl Histochem 1980; 24: 203-207.
2. Taylor CR. Principles of microscopy. In: Taylor CR, ed. Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist. Philadelphia: W B Saunders, 1986; 20-22.
3. Falini B, Pileri S, Martelli MF. Histological and Immunohistological Analysis of Human Lymphoma. Vol. 9. CRC Press, 1989; 351-419.
4. Shi S-R, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991; 39: 741-748.
5. Suurmeijer AJH. Microwave-stimulated antigen retrieval. A new method facilitating immunohistochemistry of formalin-fixed, paraffin embedded tissue. Histochem J 1992; 24: 597 (Abstract).
6. Cattoretti G, Becker HG, Key G, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992; 168: 357-363.
7. Cattoretti G, Pileri S, Parravicini C, et al. Antigen unmasking on formalin-fixed, paraffin embedded sections. J Pathol 1993; 171: 83-98.
8. Gown AM, deWever N, Battifora H. Microwave-based antigenic unmasking. A revolutionary new technique for routine immunohistochemistry. Appl Immunohistochem 1993; 1: 256-266.
9. Suurmeijer AJH, Boon ME. Notes on the application of microwaves for antigen retrieval in paraffin and plastic sections. Eur J Morphol 1993; 31: 144-150.
10. Van den Berg F, Claessen N, Mulder J, Polak M, Volkers H, Offerhaus GJA. Target unmasking fluid (TUF) for immunohistochemistry in (archival) paraffin embedded tissue. Eur Clin Lab 1993: 20: 24.
11. Leong ASY, Milios J. An assessment of the efficacy of the microwave antigen-retrieval procedure on a range of tissue antigens. Appl Immunohistochem 1993; 1: 267-274.
12. Momose H, Mehta P, Battifora H. Antigen retrieval by microwave irradiation in lead thiocyanate. Comparison with protease digestion. Appl Immunohistochem 1993; 1: 77-82.
13. Taylor CR, Shi S-R, Chaiwun B, Young L, Imam SA, Cote RJ. Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques. Hum Pathol 1994; 25: 263-270.
14. Norton AJ, Jordan S, Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol 1994; 173: 371-379.
15. Morgan JM, Navabi H, Schmid KW, Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J Pathol 1994; 174: 301-307.
16. Cattoretti G, Suurmeijer AJH. Antigen unmasking on formalin-fixed paraffin-embedded tissues using microwaves: a review. Adv Anat Pathol 1995; 2: 2-9.
17. Miller K, Auid J, Jessup E, Rhodes A, Ashton-Key M. Antigen unmasking in formalin-fixed routinely processed paraffin wax-embedded sections by pressure cooking: a comparison with microwave oven heating and traditional methods. Adv Anal Pathol 1995; 2: 60-64.
18. Shi S-R, Imam SA, Young L, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J Histochem Cytochem 1995; 43: 193-201.
19. Miller RT, Estran C. Heat-induced epitope retrieval with a pressure cooker. Suggestions for optimal use. Appl Immunohistochem 1995; 3: 190-193.
20. Pileri S. Linfomi maligni non-Hodgkin: dalla tecnica alla diagnostica. Bologna: Società Editrice Esculapio, 1985.
21. Pileri S, Falini B, Sabattini E, Bigerna B, Gherlinzoni F, Tazzari PL. Immunohistochemistry of malignant lymphomas. Advantages and limitations of the new monoclonal antibodies working in paraffin sections. Haematology 1991; 76: 226-234.
22. Harris N, Jaffe E, Stein H, et al. A Revised European-American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group. Blood 1994; 84: 1361-1392.
23. Bennett JM, Catovsky D, Daniel MT, et al. Proposals for the classification of the acute leukemias. A report of the French-American-British (FAB) co-operative group. Br J Haematol 1976; 33: 451-458.
24. Flenghi L, Bigerna B, Fizzotti M, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996; 148: 1543-1553.
25. Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984; 32: 219-229.
26. Santini D, Ceccarelli C, Mazzoleni G, Pasquinelli G, Jasonni VM, Martinelli GN. Demonstration of cytokeratin intermediate filaments in oocytes of the developing and adult human ovary. Histochemistry 1993; 99: 311-319.
27. Shin RW, Iwaki T, Kitamoto T, Tateishi J. Hydrated autoclave pretreatment enhances TAU immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissue. Lab Invest 1991; 64: 693-702.
28. Ho J, Shintaku IP, Preston M, Said JW. Can microwave antigen retrieval replace frozen section immunohistochemistry in the phenotyping of lymphoid neoplasms? A comparative study of kappa and lambda light chain staining in frozen sections, B5-fixed paraffin sections, and microwave urea antigen retrieval. Appl Immunohistochem 1994; 2: 282-286.
29. Mason JT, O'Leary TJ. Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J Histochem Cytochem 1991; 39: 225-231.