Application of heat-induced antigen retrieval to aldehyde-fixed fresh frozen sections

Journal of Histochemistry and Cytochemistry

Volumn 53, Issue 11, 2005, Pages 1421-1432

  Yamashita, Shuji ^a   Okada, Yasunori ^a  

^a KEIO UNIVERSITY   (Japan)

Author keywords

Fresh frozen section; Heat induced antigen retrieval; Immunohistochemistry; Unoccupied steroid hormone receptors

Indexed keywords

ALDEHYDE; ANTIGEN; CACODYLIC ACID; CALCIUM CHLORIDE; EDETIC ACID; ESTROGEN RECEPTOR ALPHA; FIXATIVE; FORMALDEHYDE; GLUCOCORTICOID RECEPTOR; GLUTARALDEHYDE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PHOSPHATE; POLYCLONAL ANTIBODY; PROGESTERONE RECEPTOR; STEROID RECEPTOR;

ANIMAL TISSUE; ANTIGEN RETRIEVAL; ARTICLE; CELL MEMBRANE; CELL NUCLEUS; CYTOPLASM; EXTRACELLULAR MATRIX; FROZEN SECTION; HEAT INDUCED ANTIGEN RETRIEVAL; HEATING; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; TISSUE FIXATION;

ANIMALS; CHELATING AGENTS; EDETIC ACID; ESTROGEN RECEPTOR ALPHA; FIXATIVES; FORMALDEHYDE; FROZEN SECTIONS; HEATING; IMMUNOHISTOCHEMISTRY; MICE; RATS; RATS, WISTAR; RECEPTORS, GLUCOCORTICOID;

ANIMALIA;

EID: 27244433299     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.4A6579.2005     Document Type: Article

References (30)

1. Baker JR (1944) Structure and chemical composition of the Golgi element. Q J Micr Sci 85:1-71
2. Bancroft JD (1996) Enzyme histochemistry. In Bancroft JD, Stevens A, Turner DR, eds. Theory and Practice of Histological Techniques. New York, Churchill Livingstone, 391-410
3. Cattoretti G, Pileri S, Parravicini C, Becker MH, Poggi S, Bifulco C, Key G, et al. (1993) Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J Pathol 171:83-98
4. Emoto K, Yamashita S, Okada Y (2005) Mechanisms of heat-induced antigen retrieval: does pH or ionic strength of the solution play a role for refolding antigens? J Histochem Cytochem (Epub ahead of print)
5. Evers P, Uylings HB (1997) An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative. J Neurosci Methods 72: 197-207
6. Evers P, Uylings HB, Suurmeijer AJ (1998) Antigen retrieval in formaldehyde-fixed human brain tissue. Methods 15:133-140
7. Gasc JM, Delahaye F, Baulieu EE (1989) Compared intracellular localization of the glucocorticosteroid and progesterone receptors: an immunocytochemical study. Exp Cell Res 181:492-504
8. Ino H (2003) Antigen retrieval by heating en bloc for pre-fixed frozen material. J Histochem Cytochem 51:995-1003
9. Mason JT, O'Leary TJ (1991) Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J Histochem Cytochem 39:225-229
10. McGimsey WC, Cidlowski JA, Stumpf WE, Sar M (1991) Immunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies. Endocrinology 129:3064-3072
11. Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheschek K, Moubayed P, et al. (1995) ImmunoMax. A maximized immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab Invest 73:149-156
12. Mighell AJ, Robinson PA, Hume WJ (1995) Patterns of immunoreactivity to an anti-fibronectin polyclonal antibody in formalin-fixed, paraffin-embedded oral tissues are dependent on methods of antigen retrieval. J Histochem Cytochem 43:1107-1114
13. Morgan JM, Navabi H, Jasani B (1997) Role of calcium chelation in high-temperature antigen retrieval at different pH values. J Pathol 182:233-237
14. Morgan JM, Navabi H, Schmid KW, Jasani B (1994) Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J Pathol 174:301-307
15. Pekki A, Koistinaho J, Ylikomi T, Vilja P, Westphal H, Touhimaa P (1992) Subcellular location of unoccupied and occupied glucocorticoid receptor by a new immunohistochemical technique. J Steroid Biochem Mol Biol 41:753-756
16. Pileri SA, Roncador G, Ceccarelli C, Piccioli M, Briskomatis A, Sabattini E, Ascani S, et al. (1997) Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J Pathol 183:116-123
17. Rait VK, O'Leary TJ, Mason JT (2004) Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I-Structural and functional alterations. Lab Invest 84:292-299
18. Shi SR, Chaiwun B, Young L, Cote RJ, Taylor CR (1993) Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J Histochem Cytochem 41:1599-1604
19. Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR (1999) Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: what is the signal? J Histochem Cytochem 47:463-470
20. Shi SR, Cote RJ, Taylor CR (2001) Antigen retrieval techniques: current perspectives. J Histochem Cytochem 49:931-937
21. Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-748
22. Slayden OD, Koji T, Brenner RM (1995) Microwave stabilization enhances immunocytochemical detection of estrogen receptor in frozen sections of macaque oviduct. Endocrinology. 136:4012-4021
23. Werner M, Wasielewski R, Komminoth P (1996) Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem Cell Biol 105:253-260
24. Yamashita S (2001) Histochemistry and cytochemistry of nuclear receptors. Prog Histochem Cytochem 36:91-176
25. Yamashita S, Aiso S, Shiozawa M, Yasuda K (1989) Immunohistochemical study of gamma-glutamyl transpeptidase with monoclonal antibodies. II. An immunoelectron microscopic study in rat kidney. Acta Histochem Cytochem 22:367-374
26. Yamashita S, Korach KS (1989) Immunological analysis of the biochemical properties of the uterine estrogen receptor. Biol Reprod 40:1275-1285
27. Yamashita S, Okada Y (2005) Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry. J Histochem Cytochem 53:13-21
28. Yamashita S, Sogo T, Shiozawa M, Yasuda K (1997) Immunolocalization of aldolase A subunit using monoclonal antibody in rabbit tissues. Acta Histochem Cytochem 30:601-608
29. Yamashita S, Yasuda K (1992) Monoclonal antibody to a common antigen of secretory granule membranes: intracellular localization and recycling of the antigen after secretion. J Histochem Cytochem 40:793-806
30. Yasuda K, Yamashita S, Aiso S, Shiozawa M, Komatsu T (1986) Immunohistochemical study of gamma-glutamyl transpeptidase with monoclonal antibodies. I. Preparation and characteristics of monoclonal antibodies to gamma-glutamyl transpeptidase. Acta Histochem Cytochem 19:589-600