Heat-induced antigen retrieval of epoxy sections for electron microscopy

Histology and Histopathology

Volumn 16, Issue 3, 2001, Pages 923-930

  Brorson, S H ^a  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Acrylic resin; Antigen retrieval; Epoxy resin; Immunogold; Immunohistochemistry

Indexed keywords

ACRYLIC ACID RESIN; ANTIGEN; BUFFER; EPOXY RESIN; FORMALDEHYDE; PARAFFIN;

ANTIGEN RETRIEVAL; AUTOCLAVE; CHEMICAL LABELING; ELECTRON MICROSCOPY; HEAT; HIGH TEMPERATURE PROCEDURES; HISTORY; IMMUNOHISTOCHEMISTRY; REVIEW; TECHNIQUE; THEORY; TISSUE SECTION;

EID: 0034927934     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (37)

1. A new approach to the immunostaining of epoxy-resin-embedded material
2. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry
3. 4 (Osmium)
4. Wet autoclave pretreatment for immunohistochemical demonstration of oestrogen receptors in routinely processed breast carcinoma tissue
5. Microwaves for immuno-histochemistry
6. How to examine the antigen-damaging effect of sodium ethoxide on deplasticized epoxy sections
7. Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin
8. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-White sections for large proteins
9. The fixative-dependent increase in immunogold labeling following antigen retrieval on acrylic and epoxy sections
10. Comparison of the immunogold labeling of heated and deplasticized epoxy sections on the electron microscopical level
11. Deplasticizing or etching of epoxy sections with different concentrations of sodium ethoxide to enhance the immunogold labeling
12. Increased level of immunogold labeling of epoxy sections by rising the temperature significantly beyond 100 °C in the antigen retrieval medium
13. Mechanism for antigen detection on deplasticized epoxy sections
14. The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections
15. The theoretical ratio of immunogold labeling of deplasticized epoxy sections and acrylic sections
16. Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximate the same immunogold labeling for epoxy sections as for acrylic sections without any etching
17. A new immunoelectron microscopy approach for the detection of immunoglobulin and complement deposits in epoxy-embedded renal biopsies
18. Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases
19. Antigen retrieval on epoxy sections based on tissue infiltration with moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue
20. 2-treated and heated epoxy sections
21. The choice of resins for electron immunocytochemistry
22. Polar or apolar lowicryl resin for immunolabeling
23. Fine structure preservation
24. Chemical fixation
25. Ultrastructural and electron immunohistochemical features of medullary thyroid carcinoma
26. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions
27. Colloidal gold immunostaining on deplasticized ultra-thin sections
28. Possible role of tissue-bound calcium ions in citrate-mediate high-temperature antigen retrieval
29. Immunohistochemical detection of progesterone receptors in archival breast cancer
30. LR-White embedding medium for colloidal gold methods
31. Use of hydrogen peroxide to accelerate staining of ultrathin spurr sections
32. An evaluation of antigen retrieval procedures for immunoelectron microscopic classification of amyloid deposits
33. Antigen retrieval in formalin-fixed paraffin-embedded tissue: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
34. Antigen unmasking for immunoelectron microscopy: Labelling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium
35. Comparative study of antigen retrieval heating methods: Microwave, microwave and pressure cooker, autoclave, and steamer
36. Antigen retrieval for electron microscopy using a microwave
37. A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: Demonstration of cytokeratin No. 18 in normal and neoplastic hepatocytes