Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone

Micron

Volumn 37, Issue 4, 2006, Pages 347-354

  Solberg, Lene B ^a   Melhus, Gunhild ^a   Brorson, Sverre Henning ^a   Wendel, Mikael ^b   Reinholt, Finn P ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Antigen retrieval; Electron microscopy; High pressure freezing; Immunolabeling; Lowicryl resin; Nucleobindin; Osteoadherin; Paraformaldehyde fixation

Indexed keywords

ANTIGENS; BONE; ELECTRON MICROSCOPY; FORMALDEHYDE; FREEZING; GOLD; TISSUE;

ANTIGEN RETRIEVAL; HIGH-PRESSURE FREEZING; IMMUNOLABELING; OSTEOADHERIN;

IMMUNOLOGY;

ACRYLIC ACID RESIN; CALCIUM BINDING PROTEIN; DNA BINDING PROTEIN; FORMALDEHYDE; LOWICRYL HM23; NUCLEOBINDIN; OSTEOADHERIN; PARAFORMALDEHYDE; POLYMER; PROTEOGLYCAN; SCLEROPROTEIN;

ANIMAL; ARTICLE; BONE; COMPARATIVE STUDY; FREEZE DRYING; HEAT; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; RAT; TISSUE FIXATION; ULTRASTRUCTURE;

ACRYLIC RESINS; ANIMALS; BONE AND BONES; CALCIUM-BINDING PROTEINS; DNA-BINDING PROTEINS; EXTRACELLULAR MATRIX PROTEINS; FORMALDEHYDE; FREEZE SUBSTITUTION; HEAT; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; POLYMERS; PROTEOGLYCANS; RATS; TISSUE FIXATION;

EID: 33646583167     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micron.2005.11.010     Document Type: Article

References (30)

1. Brorson S.H. Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin. Histol. Histopathol. 13 (1998) 275-281
2. Brorson S.H. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-white sections for large proteins. Micron 29 (1998) 89-95
3. Brorson S.H. Fixative-dependent increase in immunogold labeling following antigen retrieval on acrylic and epoxy sections. Biotech. Histochem. 74 (1999) 248-260
4. Brorson S.H., and Nguyen G.H. Increased level of immunogold labeling of epoxy sections by rising the temperature significantly beyond 100 °C in the antigen retrieval medium. Micron 32 (2001) 591-597
5. Brorson S.H., and Skjorten F. The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections. Micron 27 (1996) 193-201
6. Brorson S.H., Roos N., and Skjorten F. Antibody penetration into LR-White sections. Micron 25 (1994) 453-460
7. Engfeldt B., Reinholt F.P., Hultenby K., Widholm S.M., and Muller M. Ultrastructure of hypertrophic cartilage: histochemical procedures compared with high pressure freezing and freeze substitution. Calcif. Tissue Int. 55 (1994) 274-280
8. Giddings T.H. Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc. 212 (2003) 53-61
9. Hultenby K., Reinholt F.P., Oldberg A., and Heinegard D. Ultrastructural immunolocalization of osteopontin in metaphyseal and cortical bone. Matrix 11 (1991) 206-213
10. Kellenberger E., Durrenberger M., Villiger W., Carlemalm E., and Wurtz M. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J. Histochem. Cytochem. 35 (1987) 959-969
11. Leong A.S., and Sormunen R.T. Microwave procedures for electron microscopy and resin-embedded sections. Micron 29 (1998) 397-409
12. Masuda N., Ohnishi T., Kawamoto S., Monden M., and Okubo K. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res. 27 (1999) 4436-4443
13. Maunsbach A.B., and Afzelius B.A. Biomedical Electron Microscopy-Illustrated Methods and Interpretations (1999), Academic press, London pp. 104-125
14. Murk J.L., Posthuma G., Koster A.J., Geuze H.J., Verkleij A.J., Kleijmeer M.J., and Humbel B.M. Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography. J. Microsc. 212 (2003) 81-90
15. Newman G.R. LR-white embedding medium for colloidal gold methods. In: Hayat M.A. (Ed). Colloidal Gold: Principles, Methods and Applications (1989), Academic Press, New York 47-73
16. Petersson U., Somogyi E., Reinholt F.P., Karlsson T., Sugars R.V., and Wendel M. Nucleobindin is produced by bone cells and secreted into the osteoid, with a potential role as a modulator of matrix maturation. Bone 34 (2004) 949-960
17. Rait V.K., Xu L., O'Leary T.J., and Mason J.T. Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A II. Interrelationship of cross-linking, immunoreactivity, and heat treatment. Lab Invest. 84 (2004) 300-306
18. Ramstad V.E., Franzen A., Heinegard D., Wendel M., and Reinholt F.P. Ultrastructural distribution of osteoadherin in rat bone shows a pattern similar to that of bone sialoprotein. Calcif. Tissue Int. 72 (2003) 57-64
19. Robey P.G., and Boskey A.L. Extracellular matrix and biomineralization of bone. In: Favus M.J. (Ed). Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. fifth ed. (2003), American Society for Bone and Mineral Research, Washington, DC 38-46
20. Royer S.M., and Kinnamon J.C. Comparison of high-pressure freezing/freeze substitution and chemical fixation of catfish barbel taste buds. Microsc. Res. Tech. 35 (1996) 385-412
21. Sawaguchi A., McDonald K.L., and Forte J.G. High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells. J. Histochem. Cytochem. 52 (2004) 77-86
22. Senda T., Iizuka-Kogo A., and Shimomura A. Visualization of the nuclear lamina in mouse anterior pituitary cells and immunocytochemical detection of lamin A/C by quick-freeze freeze-substitution electron microscopy. J. Histochem. Cytochem. 53 (2005) 497-507
23. Shen Z., Gantcheva S., Sommarin Y., and Heinegard D. Tissue distribution of a novel cell binding protein, osteoadherin, in the rat. Matrix Biol. 18 (1999) 533-542
24. Shi S.R., Key M.E., and Kalra K.L. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39 (1991) 741-748
25. Shi S.R., Cote R.J., and Taylor C.R. Antigen retrieval immunohistochemistry: past, present, and future. J. Histochem. Cytochem. 45 (1997) 327-343
26. Shi S.R., Cote R.J., and Taylor C.R. Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem. 49 (2001) 931-937
27. Stirling J.W., and Graff P.S. Antigen unmasking for immunoelectron microscopy: labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium. J. Histochem. Cytochem. 43 (1995) 115-123
28. Studer D., Graber W., Al-Amoudi A., and Eggli P. A new approach for cryofixation by high-pressure freezing. J. Microsc. 203 (2001) 285-294
29. Yamashita S., and Okada Y. Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry. J. Histochem. Cytochem. 53 (2005) 13-21
30. Young M.F. Bone matrix proteins: their function, regulation, and relationship to osteoporosis. Osteoporosis Int. 14 Suppl 3 (2003) S35-S42