Evaluation of Azadirachta indica leaf fractions for in vitro antioxidant potential and protective effects against H2O2-lnduced oxidative damage to pBR322 DNA and red blood cells

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 15, 2009, Pages 6990-6996

  Manikandan, P ^a   Anandan, R ^b   Nagini, S ^a,b  

^a ANNAMALAI UNIVERSITY   (India)

^b CENTRAL INSTITUTE OF FISHERIES TECHNOLOGY   (India)

Author keywords

Antioxidants; Free radicals; H2O2; Lipid peroxidation; Neem leaf fractions; PBR322

Indexed keywords

ANTIOXIDANT; BACTERIAL DNA; HYDROGEN PEROXIDE; PLANT EXTRACT;

ARTICLE; AZADIRACHTA INDICA; CHEMISTRY; DNA DAMAGE; DRUG EFFECT; ERYTHROCYTE; GENETICS; HUMAN; LIPID PEROXIDATION; METABOLISM; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; PLANT LEAF; PLASMID;

ANTIOXIDANTS; AZADIRACHTA; DNA DAMAGE; DNA, BACTERIAL; ERYTHROCYTES; HUMANS; HYDROGEN PEROXIDE; LIPID PEROXIDATION; OXIDATION-REDUCTION; OXIDATIVE STRESS; PLANT EXTRACTS; PLANT LEAVES; PLASMIDS;

AZADIRACHTA INDICA;

EID: 68249098020     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf901351n     Document Type: Article

References (37)

1. Andreyev, A. Y.; Kushnareva, Y. E.; Starkov, A. A. Mitochondrial metabolism of reactive oxygen species. Biochemistry (Moscow, Russ. Fed. 2005, 70, 200-214.
2. Halliwell, B.; Gitteridge, J. M. C. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 1984, 219, 1-4.
3. Halliwell. B.; Gutteridge, J. M. C. Oxidative stress: Adaptation, damage, repair and death. In Free Radicals in Biology and Medicine; Halliwell, B., Gutteridge, J. M. C., Eds.; Oxford University Press: Oxford, U.K., 1999; pp 284-330.
4. Valko, M.; Leibfritz, D.; Moncol, J.; Cronin, M. T.; Mazur, M.; Telser. J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 2007, 39, 44-84.
5. Valko, M.; Rhodes, C. J.; Moncol, J.; Izakovic, M.; Mazur, M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem.-Biol. Interact. 2006, 160, 1-40.
6. Kasai, H.; Kawai, K. Oxidative DNA damage: Mechanisms and significance in health and disease. Antioxid. Redox Signaling 2006, 8, 981-983.
7. Waldeck, A. R.; Stocker, R. Radical-initiated lipid peroxidation in low density lipoproteins: Insights obtained from kinetic modeling. Chem. Res. Toxicol. 1996, 9, 954-964.
8. Khan, N.; Afaq. F.; Mukhtar, H. Cancer chemoprevention through dietary antioxidants: Progress and promise. Antioxid. Redox Signaling, 2008, 10, 475-510.
9. Aggarwal, B. B.; Kunnumakkara, A. B.; Harikumar, K. B.; Tharakan, S. T.; Sung, B.; Anand, P. Potential of spice-derived phytochemicals for cancer prevention. Planta Med. 2008, 74, 1560-1569.
10. Subapriya, R.; Nagini, S. Medicinal properties of neem leaves: A review. Curr. Med. Chem. Anticancer Agents 2005, 5, 149-156.
11. Sithisarn, P.; Supabphol, R.; Gritsanapan, W. Antioxidant activity of Siamese neem tree (VP1209). J. Ethnopharmacol 2005, 99, 109-112.
12. Subapriya, R.; Kumaraguruparan, R.; Abraham, S. K.; Nagini, S. Protective effects of ethanolic neem leaf extract on MNNG-induced genotoxicity and oxidative stress in mice. Drug Chem. Toxicol. 2004, 27, 15-27.
13. Subapriya, R.; Velmurugan, B.; Nagini, S. Modulation of xenobiotic-metabolizing enzymes by ethanolic neem leaf extract during hamster buccal pouch carcinogenesis. J. Exp. Clin. Cancer Res. 2005, 24, 207-214.
14. Subapriya, R.; Kumaraguruparan, R.; Nagini, S. Expression of PCNA, cytokeratin. Bcl-2 and p53 during chemoprevention of hamster buccal pouch carcinogenesis by ethanolic neem (Azadirachta indica) leaf extract. Clin. Biochem. 2006, 39, 1080-1087.
15. Subapriya, R.; Nagini, S. Ethanolic neem leaf extract protects against N-methyl-N′-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in Wistar rats. Asian Pac. J. Cancer Prev. 2003, 4, 215-223.
16. Dasgupta, T.; Banerjee, S.; Yadava, P. K.; Rao, A. R. Chemopreventive potential of Azadirachta indica (neem) leaf extract in murine carcinogenesis model systems. J. Ethnopharmacol. 2004, 92, 23-36.
17. Manikandan, P.; Vidjaya Letchoumy, P.; Gopalakrishnan, M.; Nagini, S. Evaluation of Azadirachta indica leaf fractions for in vitro antioxidant potential and in vivo modulation of biomarkers of chemoprevention in the hamster buccal pouch carcinogenesis model. Eopd Chem. Toxicol. 2008, 46, 2332-2343.
18. Chattopadhyay, R. R. Possible biochemical mode of anti-inflammatory action oí Azadirachta indica A. Juss. in rats. Indian J. Exp. Biol. 1998, 36, 418-420.
19. Marvin, C. H.; Hewitt, L. M. Analytical methods in bioassay-directed investigations of mutagenicity of air particulate material. Matal. Res. 2007, 636, 4-35.
20. Blois, M. S. Antioxidant determinations by the use of a stable free radical. Nature 1958, 26, 1199-1200.
21. Miller, N. J.; Castelluccio, C.; Tijburg, L.; Rice-Evans, C. The antioxidant properties of theaflavins and their gallate esters radical scavengers or metal chelators?. FEBS Lett. 1996, 392, 40-44.
22. Halliwell, B.; Gutteridge. J. M. C.; Arugma, O. I. The deoxyribose method: A simple 'test-tube' assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987, 165, 215-219.
23. Nishimiki, M.; Rao, N. A.; Yagi, K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun. 1972, 46, 849-853.
24. Garratt, C. J. Effect of iodination on the biological activity of insulin. Nature 1964, 28 (201), 1324-1325.
25. Oyaizu, M. Studies on products of browning reaction prepared from glucose amine, Jpn. J. Nutr. 1986, 44, 307-314.
26. Cynamon, H. A.; Isenberg, J. N.; Nguyean, C. H. Erythrocyte malondialdehyde release in vitro: A functional measure of vitamin E status. Clin. Chim. Acta 1985, 152, 169-176.
27. Kitts, D. D.; Wijewickreme, A. N.; Hu, C. Antioxidant properties of a North American ginseng extract. Mol. Cell. Biochem. 2000, 203, 1-10.
28. Meneghini, R.; Martins, E. L. Hydrogen peroxide and DNA damage. In DNA and free radicals; Halliwell, B., Aruoma, O. I., Eds.; Ellis Horwood: New York, 1993; pp 83-93.
29. Suksomtip, M.; Pongsamart, S. Protective effect against oxidation of human low-density lipoprotein and plasmid DNA strand scission of tamarind seed coat extract in vitro. LWT - Food Sci. Technol. 2008, 41, 2002-2007.
30. Lloyd, D. R.; Philips, D. Oxidative DNA damage mediated by copper(II), iron(II), and nickel(II) Fenton reactions: Evidence for site-specific mechanisms in the formation of double-strand breaks, 8-hydroxydeoxyguanosine and putative intrastand cross breaks. Mutat. Res. 1999, 424, 23-36.
31. Sithisarn, P.; Carlsen, C. U.; Andersen, M. L.; Gritsanapan, W.; Skibsted, L. H. Antioxidative effects of leaves from Azadiraehta species of different provinces. Food Chem. 2007, 104, 1539-1549.
32. Sithisarn, P.; Gritsanapan, W.; Supabphol, R.; Chavasiri, W. Antioxidative compound in the leaves of Siamese neem tree. Thai J. Pharm. Sci. 2003, 27, 73.
33. Vidya Priyadarsini, R.; Manikandan, P.; Harish Kumar, G.; Nagini, S. The neem limonoids azadirachtin and nimbolide inhibit hamster cheek pouch carcinogenesis by modulating xenobiotic-metabolizing enzymes, DNA damage, antioxidants, invasion, and angiogenesis. Free Radical Res. 2009, 43, 492-504.
34. Hanasaki, Y.; Ogawa, S.; Fukui, S. The correlation between active oxygen scavenging and antioxidative effects of flavonoids. Free Radical Biol. Med. 1994, 16, 845-850.
35. Haenen, G. R. M. M.; Bast, A. Nitric oxide scavenging of flavonoids. Methods Enzymol. 1999, 301, 490-503.
36. Morales, A. I.; Vicente-Sanchezm, C.; Santiago Sandoval, J. M.; Egido, J.; Mayoral, P.; Arevalo, M. A.; Fernandez-Tagarro, M.; Lopez-Novoa, J. M.; Perez-Barriocanal, F. Protective effect of quercetin on experimental chronic cadmium nephrotoxicity in rats is based on its antioxidant properties. Food Chem. Toxicol. 2006, 44, 2092-2100.
37. Heijnen, C. G.; Haenen, G. R. M. M.; Oostveen, R. M.; Stalpers, E. M.; Bast, A. Protection of flavonoids against lipid peroxidation: The structure activity relationship revisited. Free Radical Res. 2002, 36, 575-581.