Gene expression profiling of the host response to mycobacterium bovis infection in cattle

Transboundary and Emerging Diseases

Volumn 56, Issue 6-7, 2009, Pages 204-214

  MacHugh, D E ^a   Gormley, E ^a   Park, S D E ^a   Browne, J A ^a   Taraktsoglou, M ^a   O'Farrelly, C ^b   Meade, K G ^b  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Biomarker; Biosignature; Cattle; Gene expression; Microarray; Mycobacterium bovis; Tuberculosis

Indexed keywords

BACTERIAL ANTIGEN;

ANIMAL; ARTICLE; BOVINE TUBERCULOSIS; CATTLE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC MARKER; GENETICS; HOST PATHOGEN INTERACTION; HUMAN; IMMUNOLOGY; MICROBIOLOGY; MONONUCLEAR CELL; MYCOBACTERIUM BOVIS; ZOONOSIS;

ANIMALS; ANTIGENS, BACTERIAL; CATTLE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC MARKERS; HOST-PATHOGEN INTERACTIONS; HUMANS; LEUKOCYTES, MONONUCLEAR; MYCOBACTERIUM BOVIS; TUBERCULOSIS, BOVINE; ZOONOSES;

ANIMALIA; BOS; BOVINAE; MYCOBACTERIUM BOVIS;

EID: 68149110297     PISSN: 18651674     EISSN: 18651682     Source Type: Journal    
DOI: 10.1111/j.1865-1682.2009.01082.x     Document Type: Article

References (84)

1. Adelson, D. L., 2008 : Insights and applications from sequencing the bovine genome. Reprod. Fertil. Dev. 20, 54 60. (Pubitemid 351153487)
2. Aldwell, F. E., D. N. Wedlock, L. J. Slobbe, J. F. Griffin, B. M. Buddle G. S. Buchan, 2001 : In vitro control of Mycobacterium bovis by macrophages. Tuberculosis (Edinb.) 81, 115 123. (Pubitemid 32500097)
3. Ananiadou, S., D. B. Kell J. Tsujii, 2006 : Text mining and its potential applications in systems biology. Trends Biotechnol. 24, 571 579. (Pubitemid 44762220)
4. Blumenthal, A., J. Lauber, R. Hoffmann, M. Ernst, C. Keller, J. Buer, S. Ehlers N. Reiling, 2005 : Common and unique gene expression signatures of human macrophages in response to four strains of Mycobacterium avium that differ in their growth and persistence characteristics. Infect. Immun. 73, 3330 3341. (Pubitemid 40745874)
5. Campbell, C. J. P. Ghazal, 2004 : Molecular signatures for diagnosis of infection: application of microarray technology. J. Appl. Microbiol. 96, 18 23. (Pubitemid 38177901)
6. Cloonan, N., A. R. Forrest, G. Kolle, B. B. Gardiner, G. J. Faulkner, M. K. Brown, D. F. Taylor, A. L. Steptoe, S. Wani, G. Bethel, A. J. Robertson, A. C. Perkins, S. J. Bruce, C. C. Lee, S. S. Ranade, H. E. Peckham, J. M. Manning, K. J. McKernan S. M. Grimmond, 2008 : Stem cell transcriptome profiling via massive-scale mRNA sequencing. Nat. Methods 5, 613 619. (Pubitemid 351911866)
7. Cosma, C. L., D. R. Sherman L. Ramakrishnan, 2003 : The secret lives of the pathogenic mycobacteria. Annu. Rev. Microbiol. 57, 641 676. (Pubitemid 37392957)
8. Coussens, P. M. W. Nobis, 2002 : Bioinformatics and high throughput approach to create genomic resources for the study of bovine immunobiology. Vet. Immunol. Immunopathol. 86, 229 244. (Pubitemid 34465753)
9. Coussens, P. M., C. J. Colvin, K. Wiersma, A. Abouzied S. Sipkovsky, 2002 : Gene expression profiling of peripheral blood mononuclear cells from cattle infected with Mycobacterium paratuberculosis. Infect. Immun. 70, 5494 5502. (Pubitemid 35050983)
10. Coussens, P. M., C. J. Colvin, G. J. Rosa, J. Perez Laspiur M. D. Elftman, 2003 : Evidence for a novel gene expression program in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected cattle. Infect. Immun. 71, 6487 6498. (Pubitemid 37337709)
11. Coussens, P. M., A. Jeffers C. Colvin, 2004 : Rapid and transient activation of gene expression in peripheral blood mononuclear cells from Johne's disease positive cows exposed to Mycobacterium paratuberculosis in vitro. Microb. Pathog. 36, 93 108. (Pubitemid 37543701)
12. Elass, E., L. Aubry, M. Masson, A. Denys, Y. Guerardel, E. Maes, D. Legrand, J. Mazurier L. Kremer, 2005 : Mycobacterial lipomannan induces matrix metalloproteinase-9 expression in human macrophagic cells through a toll-like receptor 1 (TLR1)/TLR2- and CD14-dependent mechanism. Infect. Immun. 73, 7064 7068. (Pubitemid 41368883)
13. Fernandez de Mera, I. G., J. M. Perez de la Lastra, P. Ayoubi, V. Naranjo, K. M. Kocan, C. Gortazar J. de la Fuente, 2008 : Differential expression of inflammatory and immune response genes in mesenteric lymph nodes of Iberian red deer (Cervus elaphus hispanicus) naturally infected with Mycobacterium bovis. Dev. Comp. Immunol. 32, 85 91. (Pubitemid 350160922)
14. Garnier, T., K. Eiglmeier, J. C. Camus, N. Medina, H. Mansoor, M. Pryor, S. Duthoy, S. Grondin, C. Lacroix, C. Monsempe, S. Simon, B. Harris, R. Atkin, J. Doggett, R. Mayes, L. Keating, P. R. Wheeler, J. Parkhill, B. G. Barrell, S. T. Cole, S. V. Gordon R. G. Hewinson, 2003 : The complete genome sequence of Mycobacterium bovis. Proc. Natl Acad. Sci. USA 100, 7877 7882. (Pubitemid 36760063)
15. Gonzalez-Juarrero, M., L. C. Kingry, D. J. Ordway, M. Henao-Tamayo, M. Harton, R. J. Basaraba, W. H. Hanneman, I. M. Orme R. A. Slayden, 2009 : Immune response to Mycobacterium tuberculosis and identification of molecular markers of disease. Am. J. Respir. Cell Mol. Biol. 40, 398 409.
16. Gormley, E. J. D. Collins, 2000 : The development of wildlife control strategies for eradication of tuberculosis in cattle in Ireland. Tuber. Lung Dis. 80, 229 236. (Pubitemid 30842223)
17. Gormley, E., M. B. Doyle, K. McGill, E. Costello, M. Good J. D. Collins, 2004 : The effect of the tuberculin test and the consequences of a delay in blood culture on the sensitivity of a gamma-interferon assay for the detection of Mycobacterium bovis infection in cattle. Vet. Immunol. Immunopathol. 102, 413 420. (Pubitemid 39487233)
18. Heldwein, K. A. M. J. Fenton, 2002 : The role of Toll-like receptors in immunity against mycobacterial infection. Microbes Infect. 4, 937 944. (Pubitemid 34775235)
19. Heldwein, K. A., M. D. Liang, T. K. Andresen, K. E. Thomas, A. M. Marty, N. Cuesta, S. N. Vogel M. J. Fenton, 2003 : TLR2 and TLR4 serve distinct roles in the host immune response against Mycobacterium bovis BCG. J. Leukoc. Biol. 74, 277 286. (Pubitemid 38040425)
20. Hestvik, A. L., Z. Hmama Y. Av-Gay, 2005 : Mycobacterial manipulation of the host cell. FEMS Microbiol. Rev. 29, 1041 1050. (Pubitemid 41416849)
21. Hodgetts, A., M. Levin, J. S. Kroll P. R. Langford, 2007 : Biomarker discovery in infectious diseases using SELDI. Future Microbiol. 2, 35 49. (Pubitemid 46766997)
22. Hossain, H., S. Tchatalbachev T. Chakraborty, 2006 : Host gene expression profiling in pathogen-host interactions. Curr. Opin. Immunol. 18, 422 429. (Pubitemid 43947588)
23. Houben, E. N., L. Nguyen J. Pieters, 2006 : Interaction of pathogenic mycobacteria with the host immune system. Curr. Opin. Microbiol. 9, 76 85. (Pubitemid 43184340)
24. Jacobsen, M., D. Repsilber, A. Gutschmidt, A. Neher, K. Feldmann, H. J. Mollenkopf, A. Ziegler S. H. Kaufmann, 2007 : Candidate biomarkers for discrimination between infection and disease caused by Mycobacterium tuberculosis. J. Mol. Med. 85, 613 621. (Pubitemid 46876510)
25. Jacobsen, M., J. Mattow, D. Repsilber S. H. Kaufmann, 2008 : Novel strategies to identify biomarkers in tuberculosis. Biol. Chem. 389, 487 495. (Pubitemid 351874187)
26. Jenner, R. G. R. A. Young, 2005 : Insights into host responses against pathogens from transcriptional profiling. Nat. Rev. Microbiol. 3, 281 294. (Pubitemid 40443722)
27. Jensen, L. J., J. Saric P. Bork, 2006 : Literature mining for the biologist: from information retrieval to biological discovery. Nat. Rev. Genet. 7, 119 129. (Pubitemid 43128897)
28. Jozefowski, S., A. Sobota K. Kwiatkowska, 2008 : How Mycobacterium tuberculosis subverts host immune responses. BioEssays 30, 943 954.
29. Kaufmann, S. H. S. K. Parida, 2008 : Tuberculosis in Africa: learning from pathogenesis for biomarker identification. Cell Host Microbe 4, 219 228.
30. Kawai, T. S. Akira, 2005 : Pathogen recognition with Toll-like receptors. Curr. Opin. Immunol. 17, 338 344. (Pubitemid 40848534)
31. Kong, S. W., W. T. Pu P. J. Park, 2006 : A multivariate approach for integrating genome-wide expression data and biological knowledge. Bioinformatics 22, 2373 2380. (Pubitemid 44566966)
32. Koul, A., T. Herget, B. Klebl A. Ullrich, 2004 : Interplay between mycobacteria and host signalling pathways. Nat. Rev. Microbiol. 2, 189 202. (Pubitemid 39490082)
33. Krutzik, S. R. R. L. Modlin, 2004 : The role of Toll-like receptors in combating mycobacteria. Semin. Immunol. 16, 35 41. (Pubitemid 38147432)
34. Mardis, E. R., 2008 : The impact of next-generation sequencing technology on genetics. Trends Genet. 24, 133 141.
35. McNair, J., M. D. Welsh J. M. Pollock, 2007 : The immunology of bovine tuberculosis and progression toward improved disease control strategies. Vaccine 25, 5504 5511. (Pubitemid 47031374)
36. Meade, K. G., E. Gormley, S. D. Park, T. Fitzsimons, G. J. Rosa, E. Costello, J. Keane, P. M. Coussens D. E. MacHugh, 2006 : Gene expression profiling of peripheral blood mononuclear cells (PBMC) from Mycobacterium bovis infected cattle after in vitro antigenic stimulation with purified protein derivative of tuberculin (PPD). Vet. Immunol. Immunopathol. 113, 73 89. (Pubitemid 44131570)
37. Meade, K. G., E. Gormley, M. B. Doyle, T. Fitzsimons, C. O' Farrelly, E. Costello, J. Keane, Y. Zhao D. E. MacHugh, 2007 : Innate gene repression associated with Mycobacterium bovis infection in cattle: toward a gene signature of disease. BMC Genomics 8, 400.
38. Meade, K. G., E. Gormley, C. O'Farrelly, S. D. Park, E. Costello, J. Keane, Y. Zhao D. E. MacHugh, 2008 : Antigen stimulation of peripheral blood mononuclear cells from Mycobacterium bovis infected cattle yields evidence for a novel gene expression program. BMC Genomics 9, 447.
39. Medzhitov, R., 2001 : Toll-like receptors and innate immunity. Nat. Rev. Immunol. 1, 135 145. (Pubitemid 33741967)
40. Neill, S. D., J. Cassidy, J. Hanna, D. P. Mackie, J. M. Pollock, A. Clements, E. Walton D. G. Bryson, 1994 : Detection of Mycobacterium bovis infection in skin test-negative cattle with an assay for bovine interferon-gamma. Vet. Rec. 135, 134 135. (Pubitemid 24245146)
41. Netea, M. G., J. W. Van der Meer B. J. Kullberg, 2004 : Toll-like receptors as an escape mechanism from the host defense. Trends Microbiol. 12, 484 488. (Pubitemid 39370729)
42. Nguyen, L. J. Pieters, 2005 : The Trojan horse: survival tactics of pathogenic mycobacteria in macrophages. Trends Cell Biol. 15, 269 276. (Pubitemid 40603896)
43. Noss, E. H., C. V. Harding W. H. Boom, 2000 : Mycobacterium tuberculosis inhibits MHC class II antigen processing in murine bone marrow macrophages. Cell. Immunol. 201, 63 74. (Pubitemid 30337710)
44. Noss, E. H., R. K. Pai, T. J. Sellati, J. D. Radolf, J. Belisle, D. T. Golenbock, W. H. Boom C. V. Harding, 2001 : Toll-like receptor 2-dependent inhibition of macrophage class II MHC expression and antigen processing by 19-kDa lipoprotein of Mycobacterium tuberculosis. J. Immunol. 167, 910 918. (Pubitemid 32660847)
45. O'Reilly, L. M. C. J. Daborn, 1995 : The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tuber. Lung Dis. 76 (Suppl. 1 1 46.
46. Perry, B. D., T. F. Randolph, J. J. McDermott, K. R. Sones P. K. Thornton, 2002 : Investing in Animal Health Research to Alleviate Poverty. ILRI (International Livestock Research Institute), Nairobi. p. 148.
47. Phillips, C. J., C. R. Foster, P. A. Morris R. Teverson, 2003 : The transmission of Mycobacterium bovis infection to cattle. Res. Vet. Sci. 74, 1 15. (Pubitemid 36175763)
48. Pieters, J., 2008 : Mycobacterium tuberculosis and the macrophage: maintaining a balance. Cell Host Microbe 3, 399 407. (Pubitemid 351773101)
49. Pollock, J. M. S. D. Neill, 2002 : Mycobacterium bovis infection and tuberculosis in cattle. Vet. J. 163, 115 127. (Pubitemid 37555102)
50. Pollock, J. M., B. M. Buddle P. Andersen, 2001a : Towards more accurate diagnosis of bovine tuberculosis using defined antigens. Tuberculosis (Edinb.) 81, 65 69. (Pubitemid 32500091)
51. Pollock, J. M., J. McNair, M. D. Welsh, R. M. Girvin, H. E. Kennedy, D. P. Mackie S. D. Neill, 2001b : Immune responses in bovine tuberculosis. Tuberculosis (Edinb.) 81, 103 107. (Pubitemid 32500095)
52. Pollock, J. M., M. D. Welsh J. McNair, 2005 : Immune responses in bovine tuberculosis: towards new strategies for the diagnosis and control of disease. Vet. Immunol. Immunopathol. 108, 37 43. (Pubitemid 41317064)
53. Quesniaux, V., C. Fremond, M. Jacobs, S. Parida, D. Nicolle, V. Yeremeev, F. Bihl, F. Erard, T. Botha, M. Drennan, M. N. Soler, M. Le Bert, B. Schnyder B. Ryffel, 2004 : Toll-like receptor pathways in the immune responses to mycobacteria. Microbes Infect. 6, 946 959. (Pubitemid 39061217)
54. Raju, B., Y. Hoshino, I. Belitskaya-Levy, R. Dawson, S. Ress, J. A. Gold, R. Condos, R. Pine, S. Brown, A. Nolan, W. N. Rom M. D. Weiden, 2008 : Gene expression profiles of bronchoalveolar cells in pulmonary TB. Tuberculosis (Edinb.) 88, 39 51. (Pubitemid 350267356)
55. Reiling, N., C. Holscher, A. Fehrenbach, S. Kroger, C. J. Kirschning, S. Goyert S. Ehlers, 2002 : Cutting edge: Toll-like receptor (TLR)2- and TLR4-mediated pathogen recognition in resistance to airborne infection with Mycobacterium tuberculosis. J. Immunol. 169, 3480 3484.
56. Reiling, N., S. Ehlers C. Holscher, 2008 : MyDths and un-TOLLed truths: sensor, instructive and effector immunity to tuberculosis. Immunol. Lett. 116, 15 23.
57. Reviriego Gordejo, F. J. J. P. Vermeersch, 2006 : Towards eradication of bovine tuberculosis in the European Union. Vet. Microbiol. 112, 101 109. (Pubitemid 43182742)
58. Rhodes, S. G., B. M. Buddle, R. G. Hewinson H. M. Vordermeier, 2000 : Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease. Immunology 99, 195 202. (Pubitemid 30093663)
59. Rothel, J. S., S. L. Jones, L. A. Corner, J. C. Cox P. R. Wood, 1992 : The gamma-interferon assay for diagnosis of bovine tuberculosis in cattle: conditions affecting the production of gamma-interferon in whole blood culture. Aust. Vet. J. 69, 1 4.
60. Russell, D. G., S. Sturgill-Koszycki, T. Vanheyningen, H. Collins U. E. Schaible, 1997 : Why intracellular parasitism need not be a degrading experience for Mycobacterium. Philos. Trans. R. Soc. Lond., B Biol. Sci. 352, 1303 1310. (Pubitemid 27434094)
61. Rzhetsky, A., M. Seringhaus M. Gerstein, 2008 : Seeking a new biology through text mining. Cell 134, 9 13. (Pubitemid 351905746)
62. Sartain, M. J., R. A. Slayden, K. K. Singh, S. Laal J. T. Belisle, 2006 : Disease state differentiation and identification of tuberculosis biomarkers via native antigen array profiling. Mol. Cell Proteomics 5, 2102 2113. (Pubitemid 44817022)
63. Shendure, J., 2008 : The beginning of the end for microarrays? Nat. Methods 5, 585 587. (Pubitemid 351911858)
64. Skovgaard, K., S. N. Grell, P. M. Heegaard, G. Jungersen, C. B. Pudrith P. M. Coussens, 2006 : Differential expression of genes encoding CD30L and P-selectin in cattle with Johne's disease: progress toward a diagnostic gene expression signature. Vet. Immunol. Immunopathol. 112, 210 224. (Pubitemid 43975143)
65. Snelling, W. M., R. Chiu, J. E. Schein, M. Hobbs, C. A. Abbey, D. L. Adelson, J. Aerts, G. L. Bennett, I. E. Bosdet, M. Boussaha, R. Brauning, A. R. Caetano, M. M. Costa, A. M. Crawford, B. P. Dalrymple, A. Eggen, A. Everts-van der Wind, S. Floriot, M. Gautier, C. A. Gill, R. D. Green, R. Holt, O. Jann, S. J. Jones, S. M. Kappes, J. W. Keele, P. J. de Jong, D. M. Larkin, H. A. Lewin, J. C. McEwan, S. McKay, M. A. Marra, C. A. Mathewson, L. K. Matukumalli, S. S. Moore, B. Murdoch, F. W. Nicholas, K. Osoegawa, A. Roy, H. Salih, L. Schibler, R. D. Schnabel, L. Silveri, L. C. Skow, T. P. Smith, T. S. Sonstegard, J. F. Taylor, R. Tellam, C. P. Van Tassell, J. L. Williams, J. E. Womack, N. H. Wye, G. Yang S. Zhao, 2007 : A physical map of the bovine genome. Genome Biol. 8, R165.
66. Sundaramurthy, V. J. Pieters, 2007 : Interactions of pathogenic mycobacteria with host macrophages. Microbes Infect. 9, 1671 1679. (Pubitemid 350217085)
67. Thacker, T. C., M. V. Palmer W. R. Waters, 2007 : Associations between cytokine gene expression and pathology in Mycobacterium bovis infected cattle. Vet. Immunol. Immunopathol. 119, 204 213. (Pubitemid 47368668)
68. The Gene Ontology Consortium 2008 : The Gene Ontology Project in 2008. Nucleic Acids Res. 36, D440 D444.
69. Thuong, N. T., S. J. Dunstan, T. T. Chau, V. Thorsson, C. P. Simmons, N. T. Quyen, G. E. Thwaites, N. Thi Ngoc Lan, M. Hibberd, Y. Y. Teo, M. Seielstad, A. Aderem, J. J. Farrar T. R. Hawn, 2008 : Identification of tuberculosis susceptibility genes with human macrophage gene expression profiles. PLoS Pathog. 4, e1000229.
70. Tobian, A. A., N. S. Potter, L. Ramachandra, R. K. Pai, M. Convery, W. H. Boom C. V. Harding, 2003 : Alternate class I MHC antigen processing is inhibited by Toll-like receptor signaling pathogen-associated molecular patterns: Mycobacterium tuberculosis 19-kDa lipoprotein, CpG DNA, and lipopolysaccharide. J. Immunol. 171, 1413 1422. (Pubitemid 36900075)
71. Tree, J. A., M. J. Elmore, S. Javed, A. Williams P. D. Marsh, 2006 : Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination. Infect. Immun. 74, 1436 1441. (Pubitemid 43185590)
72. Wang, J. P., S. E. Rought, J. Corbeil D. G. Guiney, 2003 : Gene expression profiling detects patterns of human macrophage responses following Mycobacterium tuberculosis infection. FEMS Immunol. Med. Microbiol. 39, 163 172. (Pubitemid 37415082)
73. Wang, Z., M. Gerstein M. Snyder, 2009 : RNA-Seq: a revolutionary tool for transcriptomics. Nat. Rev. Genet. 10, 57 63.
74. Wedlock, D. N., M. A. Skinner, G. W. de Lisle B. M. Buddle, 2002 : Control of Mycobacterium bovis infections and the risk to human populations. Microbes Infect. 4, 471 480. (Pubitemid 34270684)
75. Wedlock, D. N., R. P. Kawakami, J. Koach, B. M. Buddle D. M. Collins, 2006 : Differences of gene expression in bovine alveolar macrophages infected with virulent and attenuated isogenic strains of Mycobacterium bovis. Int. Immunopharmacol. 6, 957 961.
76. Werling, D. T. J. Coffey, 2007 : Pattern recognition receptors in companion and farm animals - the key to unlocking the door to animal disease? Vet. J. 174, 240 251. (Pubitemid 47424135)
77. Werling, D. T. W. Jungi, 2003 : TOLL-like receptors linking innate and adaptive immune response. Vet. Immunol. Immunopathol. 91, 1 12. (Pubitemid 36042793)
78. Werling, D., J. Piercy T. J. Coffey, 2006 : Expression of TOLL-like receptors (TLR) by bovine antigen-presenting cells-potential role in pathogen discrimination? Vet. Immunol. Immunopathol. 112, 2 11. (Pubitemid 43817193)
79. Widdison, S., L. J. Schreuder, B. Villarreal-Ramos, C. J. Howard, M. Watson T. J. Coffey, 2006 : Cytokine expression profiles of bovine lymph nodes: effects of Mycobacterium bovis infection and bacille Calmette-Guerin vaccination. Clin. Exp. Immunol. 144, 281 289.
80. Widdison, S., M. Watson, J. Piercy, C. Howard T. J. Coffey, 2008 : Granulocyte chemotactic properties of M. tuberculosis versus M. bovis-infected bovine alveolar macrophages. Mol. Immunol. 45, 740 749. (Pubitemid 47498579)
81. Womack, J. E., 2006 : The bovine genome. Genome Dyn. 2, 69 78.
82. Xu, Y., J. Xie, Y. Li, J. Yue, J. Chen, L. Chunyu H. Wang, 2003 : Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis. Chin. Med. J. (Engl.) 116, 1070 1073. (Pubitemid 36975163)
83. Yao, J., J. L. Burton, P. Saama, S. Sipkovsky P. M. Coussens, 2001 : Generation of EST and cDNA microarray resources for the study of bovine immunobiology. Acta Vet. Scand. 42, 391 405. (Pubitemid 33642117)
84. Zinsstag, J., E. Schelling, F. Roth R. Kazwala, 2006 : Economics of bovine tuberculosis. In : Thoen, C. O., J. H. Steele M. J. Gilsdorf (eds Mycobacterium bovis Infection in Animals and Humans, pp. 68 83. Blackwell Pub, Ames, Iowa; Oxford.