Development of quantitative duplex real-time PCR method for screening analysis of genetically modified maize

Journal of the Food Hygienic Society of Japan

Volumn 50, Issue 3, 2009, Pages 117-125

  Oguchi, Taichi ^a,f   Onishi, Mari ^b   Minegishi, Yasutaka ^c   Kurosawa, Yasunori ^a   Kasahara, Masaki ^a,d   Akiyama, Hiroshi ^e   Teshima, Reiko ^e   Futo, Satoshi ^b   Furui, Satoshi ^a   Hino, Akihiro ^a   Kitta, Kazumi ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b FASMAC Co   (Japan)

^c NIPPON GENE Co Ltd   (Japan)

^d FOOD AND AGRICULTURAL MATERIALS INSPECTION CENTER   (Japan)

^e NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^f NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Duplex real time PCR; Genetically modified (GM); Maize (Zea mays)

Indexed keywords

ARTICLE; FOOD ANALYSIS; GENETICALLY MODIFIED FOOD; GENETICS; MAIZE; METHODOLOGY; POLYMERASE CHAIN REACTION;

FOOD ANALYSIS; FOOD, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; ZEA MAYS;

CAULIFLOWER MOSAIC VIRUS; ZEA MAYS;

EID: 68049137334     PISSN: 00156426     EISSN: None     Source Type: Journal    
DOI: 10.3358/shokueishi.50.117     Document Type: Article

References (20)

1. James, C. Global status of commercialized biotech/GM crops 2007. ISAAA Briefs, Vol. 37, ISAAA, Ithaca, NY, 2007.
2. Hino, A. Safety assessment and public concerns for genetically modified food products: The Japanese experience. Toxicol. Pathol, 30, 126-128 (2002).
3. Ahmed, F. E. Detection of genetically modified organisms in foods. Trends Biotechnol., 20, 215-223 (2002).
4. Hoist-Jensen, A., Ronning, S. B., Lovseth, A., Berdal, K. G. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem., 375, 985-993 (2003).
5. Rodoríguez-Lázaro, D., Lombard, B., Smith, H., Rzezutka, A., D'agostino, M., Helmuth, R., Schroeter, A., Malorny, B., Miko, A., Guerra, B., Davison, J., Kobilinsky, A., Herńandes, M., Bertheau, Y., Cook, N. Trends in analytical methodology in food safety and quality: Monitoring microorganisms and genetically modified organisms. Trands Food Sci. Tech., 18, 306-319 (2007).
6. Matsuoka, T., Kawashima, Y., Akiyama, H., Miura, H., Goda, Y., Kusakabe, Y., Isshiki, K., Toyoda, M., Hino, A. A method of detecting recombinant DNAs from four lines of genetically modified maize. J. Food Hyg. Soc. Japan., 41, 137-143 (2000).
7. Matsuoka, T., Kuribara, H., Takubo, K., Akiyama, H., Miura, H., Goda, Y., Kusakabe, Y., Isshiki, K., Toyoda, M., Hino, A. Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays). J. Agric. Food Chem., 50, 2100-2109 (2002).
8. Watanabe, T., Kuribara, H., Mishima, T., Kikuchi, H., Kodama, T., Futo, S., Kasama, K., Toyota, A., Nouno, M., Saita, A., Takahashi, K., Hino, A., Akiyama, H., Maitani, T. New qualitative detection methods of genetically modified potatoes. Biol. Pharm. Bull., 27, 1333-1339 (2004).
9. Onishi, M., Matsuoka, T., Kodama, T., Kashiwaba, K., Futo, S., Akiyama, H., Maitani, T., Furui, S., Oguchi, T., Hino, A. Development of a multiplex polymerase chain reaction method for simultaneous detection of eight events of genetically modified maize. J. Agric. Food Chem., 53, 9713-9721 (2005).
10. Watanabe, T., Tokishita, S., Spiegelhalter, F., Furui, S., Kitta, K., Hino, A., Matsuda, R., Akiyama, H., Maitani, T. Development and evaluation of event-specific qualitative PCR methods for genetically modified Bt10 maize. J. Agric. Food Chem., 55, 1274-1279 (2007).
11. Watanabe, T., Shiramasa, Y., Furui, S., Kitta, K., Minegishi, Y., Akiyama, H., Maitani, T. Development and evaluation of qualitative detection methods for unapproved genetically modified rice (LLrice). J. Food Hyg. Soc. Japan., 48, 170-178 (2007).
12. Kuribara, H., Shindo, Y., Matsuoka, T., Takubo, K., Futo, S., Aoki, N., Hirao, T., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int., 85, 1077-1089 (2002).
13. Oguchi, T., Onishi, M., Chikagawa, Y., Minegishi, Y., Kodama, T., Akiyama, H., Ohno, Y., Futo, S., Hino, A., Furui, S., Kitta, K. Development of event-specific quantitation method for GA21 maize, which is a GM event without CaMV35S promoter. J. Food Hyg. Soc. Japan., 49, 16-22 (2008).
14. Akiyama, H., Watanabe, T., Wakabayashi, K., Nakade, S., Yasui, S., Sakata, K., Chiba, R., Spiegelhalter, F., Hino, A., Maitani, T. Quantitative detection system for maize sample containing combined-trait genetically modified maize. Anal. Chem., 77, 7421-7428 (2005).
15. Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y.F Toyoda, M., Hino, A. Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J. AOAC Int., 85, 1119-1126 (2002).
16. Yoshimura, T., Kuribara, H., Matsuoka, T., Kodama, T., Iida, M., Watanabe, T., Akiyama, H., Maitani, T., Furui, S., Hino, A. Applicability of the quantification of genetically modified organisms to foods processed from maize and soy. J. Agric. Food Chem., 53, 2052-2059 (2005).
17. Fernandez, S., Charles-Delobel, C., Geldreich, A., Berthier, G., Boyer, F., Collonnier, C., Coue-Philippe, G., Diolez, A., Duplan, M. N., Kebdani, N., Romaniuk, M., Feinberg, M., Bertheau, Y. Quantification of the 35S promoter in DNA extracts from genetically modified organisms using realtime polymerase chain reaction and specificity assessment on various genetically modified organisms, Part I: Operating procedure. J. AOAC Int., 88, 547-557 (2005).
18. Cankar, K., Ravnikar, M., Zel, J., Gruden, K., Toplak, N. Real-time polymerase chain reaction detection of cauliflower mosaic virus to complement the 35S screening Assay for genetically modified organisms. J. AOAC Int., 88, 814-822 (2005).
19. Cankar, K., Chau vensy-Ancel, V., Fortabat, M.N., Gruden, K., Kobilinsky, A., Zel, J., Bertheau, Y. Detection of non-authorized genetically modified organisms using differential quantitative polymerase chain reaction: Application to 35S in maize. Anal Biochem., 376, 189-199 (2008).
20. Thompson, M., Ellison, S. L. R., Wood, R. Harmonized guidelines for single-laboratory validation of methods of analysis. Pure Appl. Chem., 74, 835-855 (2002).