Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize

Analytical Biochemistry

Volumn 376, Issue 2, 2008, Pages 189-199

  Cankar, Katarina ^a   Chauvensy Ancel, Valérie ^b   Fortabat, Marie Noelle ^b   Gruden, Kristina ^a   Kobilinsky, André ^c   Žel, Jana ^a   Bertheau, Yves ^b  

^a NATIONAL INSTITUTE OF BIOLOGY   (Slovenia)

^b CEMAGREF   (France)

^c UR341 National Agronomical Research Institute (INRA)   (France)

Author keywords

35S Promoter; Differential quantitative PCR; Differential quantitative real time PCR; Genetically modified organisms; GMO; Maize; Nonauthorized genetically modified organism; P35S

Indexed keywords

GENETICALLY MODIFIED PLANTS; VIRUSES;

35S PROMOTER; GENETICALLY MODIFIED ORGANISMS; MAIZE; P35S; QUANTITATIVE PCR; QUANTITATIVE REAL TIME PCR;

POLYMERASE CHAIN REACTION;

ARTICLE; CAULIFLOWER MOSAIC VIRUS; GENE SEQUENCE; MAIZE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; QUANTITATIVE ANALYSIS; STATISTICAL MODEL; TRANSGENIC ORGANISM; TRANSGENIC PLANT;

CAULIFLOWER MOSAIC VIRUS; ZEA MAYS;

EID: 41949125089     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.02.013     Document Type: Article

References (28)

1. C. James, Executive Summary of Global Status of Commercialized Biotech/GM Crops: 2005. ISAAA Briefs No. 34. ISAAA: Ithaca, NY.
2. Agbios GM database (http://www.agbios.com/).
3. European Commission. Regulation (EC) No. 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Off. J. Eur. Union 2003, L 268, 1-23.
4. Hernandez M., Rodriguez-Lazaro D., and Ferrando A. Current Methodology for Detection, Identification and Quantification of Genetically Modified Organisms. Curr. Anal. Chem. 1 (2005) 203-221
5. European Commission. Commission Regulation (EC) No. 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No. 1829/2003 of the European Parliament and of the Council as regards the application for the authorisation of new genetically modified food and feed, the notification of existing products and adventitious or technically unavoidable presence of genetically modified material which has benefited from a favourable risk evaluation. Off. J. Eur. Union 2004, L 102, 14-25.
6. Dorey E. Taco dispute underscores need for standardized tests. Nat. Biotechnol. 18 (2000) 1136-1137
7. Fox J.L. EPA re-evaluates StarLink license. Nat. Biotechnol. 19 (2001) 11
8. Cockburn A. Commercial plant breeding: What is in the biotech pipeline?. J. Commer. Biotechnol. 10 (2004) 209-223
9. The Pew Initiative on Food and Biotechnology, Harvest on Horizon: Future Uses Of Agricultural Biotechnology. University of Richmond, VA, September 2001.
10. Vogel G. Tracing the transatlantic spread of GM rice. Science 313 (2006) 1714
11. Aarts H.J.M., Rie J.P.Fv., and Kok E.J. Traceability of genetically modified organisms. Expert Rev. Mol. Diagn. 2 (2002) 69-77
12. Made D., Degner C., and Grohmann L. Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice. Eur. Food Res. Technol. 224 (2006) 271-278
13. Germini A., Rossi S., Zanetti A., Corradini R., Fogher C., and Marchelli R R. Development of a peptide nucleic acid array platform for the detection of genetically modified organisms in food. J. Agric. Food Chem. 53 (2005) 3958-3962
14. Kok E.J., Aarts H.J., Van Hoef A.M., and Kuiper H.A. DNA methods: critical review of innovative approaches. J. AOAC Int. 85 (2002) 797-800
15. Leimanis S., Hernández M., Fernández S., Boyer F., Burns M., Bruderer S., Glouden T., Harris N., Kaeppeli O., Philipp P., Pla M., Puigdomènech P., Vaitilingom M., Bertheau Y., and Remacle J. A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients. Plant Mol. Biol. 61 (2006) 123-139
16. Nesvold H., Kristoffersen A.B., Holst-Jensen A., and Berdal K.G. Design of a DNA chip for detection of unknown genetically modified organisms (GMOs). Bioinformatics 21 (2005) 1917-1926
17. Wolf C., Scherzinger M., Wurz A., Pauli U., Hübner P., and Lüthy J. Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for false-positive 35S-promoter screening results. Eur. Food Res. Technol. 210 (2000) 367-372
18. Cankar K., Ravnikar M., Zel J., Toplak N., and Gruden K. Real-time polymerase chain reaction detection of cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J. AOAC Int. 88 (2005) 814-822
19. Franck A., Guilley H., Jonard G., Richards K., and Hirth L. Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21 (1980) 285-294
20. Holck A., Vaïtilingom M., Didierjean L., and Rudi K. 5′-Nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 maize. Eur. Food Res. Technol. 214 (2002) 449-453
21. Collonnier C., Schattner A., Berthier G., Boyer F., Coue-Philippe G., Diolez A., Duplan M.N., Fernandez S., Kebdani N., Kobilinsky A., Romaniuk M., de Beuckeleer M., de Loose M., Windels P., and Bertheau Y. Characterization and Event Specific detection by Quantitative Real-Time PCR of T25 Maize Insert. J. AOAC Int. 88 (2005) 536-546
22. Hernandez M., Duplan M.N., Berthier G., Vaitilingom M., Hauser W., Freyer R., Pla M., and Bertheau Y. Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of Zea mays L. J. Agric. Food Chem. 52 (2004) 4632-4637
23. Fernandez S., Charles-Delobel C., Geldreich A., Berthier G., Boyer F., Collonnier C., Coue-Philippe G., Diolez A., Duplan M.N., Kebdani N., Romaniuk M., Feinberg M., and Bertheau Y. Quantification of the P35S Promotor in DNA extracts from Genetically Modified Organisms using Real-Time Polymerase Chain Reaction and Specificity assessment on various Genetically Modified Organisms, Part I: Operating Procedure. J. AOAC Int. 88 (2005) 547-557
24. Finney D.J. Statistical method in biological assay (1971), Griffin, London
25. Van Der Vaart A.W. Asymptotic Statistics (1998), Cambridge University Press
26. R Development Core Team: R: A Language and Environment for Statistical Computing (manual). R Foundation for Statistical Computing, Vienna, Austria 2005 ({ISBN} 3-900051-07-0, http://www.R-project.org).
27. S. Bruderer, K.E. Leitner, Genetically Modified (GM) Crops: molecular and regulatory details, Basel, Švica, BATS, Centre for biosafety and sustainability (http://www.bats.ch/gmo-watch/) 2003.
28. Bates D.M., and Watts D.G. Nonlinear Regression Analysis and Its Applications (1988), Wiley