RB69 DNA polymerase mutants with expanded nascent base-pair-binding pockets are highly efficient but have reduced base selectivity

Biochemistry

Volumn 48, Issue 29, 2009, Pages 6940-6950

  Zhang, Hong ^a   Beckman, Jeff ^a   Wang, Jimin ^a   Konigsberg, William ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BASE PAIRS; BASE SELECTIVITY; BINDING POCKETS; CATALYTIC CENTER; DNA POLYMERASE; DOUBLE MUTANTS; INSERTION RATES; KINETIC DATA; LESION BYPASS; MINIMAL EFFECTS; NEGATIVE SELECTION; SINGLE MUTANT; SULFOLOBUS SOLFATARICUS; TRANSLESION; TRIPLE MUTANTS; WILD TYPES;

BIOCHEMISTRY; GENES; NUCLEIC ACIDS;

DNA;

ALANINE; DNA POLYMERASE; GLYCINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE; BASE PAIRING; CONTROLLED STUDY; DNA REPLICATION; MUTANT; NONHUMAN; PRIORITY JOURNAL; STEADY STATE;

BACTERIOPHAGES; BASE PAIRING; DNA-DIRECTED DNA POLYMERASE; KINETICS; MODELS, MOLECULAR; MUTATION; SPECTROMETRY, FLUORESCENCE;

SULFOLOBUS SOLFATARICUS;

EID: 67651218926     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900422b     Document Type: Article

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