Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: Identification and mutational analysis of the active site residues

Biochemistry

Volumn 48, Issue 29, 2009, Pages 6921-6931

  Jiang, Ming ^a   Chen, Xiaolei ^a   Wu, Xian Hui ^b   Chen, Minjiao ^a   Wu, Yun Dong ^b   Guo, Zhihong ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

^b PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE RESIDUES; ARGININE RESIDUE; ASPARTATE; BIOSYNTHETIC PATHWAY; CARBOXYLATE GROUPS; CATALYTIC BASE; CATALYTIC FUNCTIONS; CATALYTIC MECHANISMS; CYCLOHEXADIENE; ELIMINATION REACTION; ENZYMATIC REACTION; EVOLUTIONARY ORIGIN; GENERAL BASE; HISTIDINE RESIDUES; HYDROLASES; HYDROXYL GROUPS; IONIC SALTS; KINETIC PROPERTIES; MICHAELIS COMPLEX; MUTATIONAL ANALYSIS; OXYANION HOLE; PH DEPENDENCE; POLAR GROUPS; PROTON ABSTRACTION; PROTON TRANSFER REACTIONS; PYRUVATE; SEQUENCE ANALYSIS; SERINE PROTEASE; SERINE RESIDUES; SIDE-CHAIN; STEADY-STATE KINETICS; STRUCTURAL MODELING; SYNTHASE;

ABSTRACTING; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; CARBOXYLATION; CATALYSIS; CHARGE TRANSFER; ENZYMES; ESCHERICHIA COLI; HYDROGEN; HYDROGEN BONDS; PROTON TRANSFER; PROTONS; QUANTUM CHEMISTRY;

AMINO ACIDS;

2 SUCCINYL 6 HYDROXY 2,4 CYCLOHEXADIENE 1 CARBOXYLATE SYNTHASE; ARGININE; HYDROLASE; MENAQUINONE; SYNTHETASE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROGEN BOND; MUTATIONAL ANALYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTON TRANSPORT; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STEADY STATE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOCATALYSIS; CATALYTIC DOMAIN; DNA PRIMERS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXO-ACID-LYASES; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTROSCOPY, NEAR-INFRARED; VITAMIN K 2;

ESCHERICHIA COLI;

EID: 67651208493     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900897h     Document Type: Article

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