Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN′ by NMR

Protein Science

Volumn 12, Issue 4, 2003, Pages 794-810

  Day, Regina M ^a   Thalhauser, Craig J ^b   Sudmeier, James L ^b   Vincent, Matthew P ^b   Torchilin, Ekaterina V ^b   Sanford, David G ^b   Bachovchin, Christopher W ^b   Bachovchin, William W ^b  

^a Tupper Research Institute   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

15N NMR; Histidine; Hydrogen bond; NMR spectroscopy; Peptide boronic acid inhibitor; Serine protease; Tautomerism

Indexed keywords

CARBONYL DERIVATIVE; HISTIDINE; IMIDAZOLE; SERINE PROTEINASE; SUBTILISIN;

ACID BASE BALANCE; AQUEOUS SOLUTION; ARTICLE; BACILLUS SUBTILIS; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL BOND; CHEMICAL REACTION; COMPLEX FORMATION; COMPUTER SIMULATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; HYDROGEN BOND; KINETICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; TAUTOMERIC SHIFT; TITRIMETRY;

ACID-BASE EQUILIBRIUM; BACILLUS SUBTILIS; HISTIDINE; HYDROGEN BONDING; ISOMERISM; MAGNETIC RESONANCE SPECTROSCOPY; SUBTILISINS;

BACILLUS SUBTILIS; POSIBACTERIA;

EID: 0037379161     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0235203     Document Type: Article

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