Impact of clarification strategy on chromatographic separations: Pre-processing of cell homogenates

Biotechnology and Bioengineering

Volumn 100, Issue 5, 2008, Pages 941-949

  Bracewell, D G ^a   Boychyn, M ^a   Baldascini, H ^a   Storey, S A ^a   Bulmer, M ^b   More, J ^b   Hoare, M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b BIO PRODUCTS LABORATORY   (United Kingdom)

Author keywords

Centrifugation; Chromatography; Clarification; Filtration; Fouling; Hydrophobic interaction; Lipids

Indexed keywords

AGGLOMERATION; CENTRIFUGATION; CENTRIFUGES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CLARIFICATION; CLARIFIERS; COLUMNS (STRUCTURAL); HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; LIPIDS; LIQUID CHROMATOGRAPHY; ORGANIC COMPOUNDS; ORGANIC SOLVENTS; POROUS MATERIALS; SEPARATION; SUSPENSIONS (COMPONENTS);

ALCOHOL DEHYDROGENASE; BIO PROCESSES; CELL HOMOGENATES; CHROMATOGRAPHIC SEPARATIONS; COLUMN CAPACITY; FOULING; HYDROPHOBIC INTERACTION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; INTERACTIVE EFFECTS; INTRACELLULAR PROTEINS; PRE-PROCESSING; SACCHAROMYCES CEREVISIAE; SOLID-LIQUID SEPARATION; TARGET MOLECULES;

FILTRATION;

ALCOHOL DEHYDROGENASE; CELL PROTEIN; ERGOSTEROL; LIPID; ORGANIC SOLVENT; PHOSPHATIDYLCHOLINE; CELL EXTRACT;

ARTICLE; BIOPROCESS; CELL AGGREGATION; CELL FREE SYSTEM; CENTRIFUGATION; CHROMATOGRAPHY; FILTRATION; MEDICAL RESEARCH; NONHUMAN; SACCHAROMYCES CEREVISIAE; SEPARATION TECHNIQUE; SUPERNATANT; YEAST; CHEMISTRY; LABORATORY DIAGNOSIS; METHODOLOGY;

SACCHAROMYCES CEREVISIAE;

CELL EXTRACTS; CHROMATOGRAPHY; SPECIMEN HANDLING;

EID: 48449088099     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21823     Document Type: Article

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