Formamide as a denaturant for bisulfite conversion of genomic DNA: Bisulfite sequencing of the GSTPi and RARβ2 genes of 43 formalin-fixed paraffin-embedded prostate cancer specimens

Analytical Biochemistry

Volumn 392, Issue 2, 2009, Pages 117-125

  Zon, Gerald ^a   Barker, Melissa A ^a   Kaur, Pavinder ^b   Groshen, Susan ^c   Jones, Lawrence W ^b   Imam, S Ashraf ^b   Boyd, Victoria L ^a  

^a APPLIED BIOSYSTEMS   (United States)

^b HUNTINGTON MEDICAL RESEARCH INSTITUTES   (United States)

^c UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Bisulfite sequencing; Bisulfite converted DNA; DNA methylation; DNA RNA extraction; Formalin fixed paraffin embedded specimens; Prostate cancer

Indexed keywords

ALKYLATION; AMIDES; BIOPSY; DENATURATION; DNA; EXTRACTION; FORMALDEHYDE; GENE ENCODING; METHYLATION; PARAFFINS; POLYMERASE CHAIN REACTION; SODIUM HYDROXIDE; SULFONATION; TISSUE; UREA; UROLOGY;

BISULFITE-SEQUENCING; DEGREE OF RELIABILITY; DNA METHYLATION; GLUTATHIONE S-TRANSFERASES; PROSTATE CANCER CELLS; PROSTATE CANCERS; RETINOIC ACID RECEPTORS; SENSITIVITY AND SPECIFICITY;

DISEASES;

BISULFITE; FORMALDEHYDE; FORMAMIDE; GENOMIC DNA; GLUTATHIONE TRANSFERASE P1; PARAFFIN; RETINOIC ACID RECEPTOR BETA; SODIUM HYDROXIDE;

ARTICLE; CANCER CELL; CELL COUNT; CLINICAL ARTICLE; CONTROLLED STUDY; CPG ISLAND; DNA DENATURATION; DNA METHYLATION; DNA SEQUENCE; EMBEDDING; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROSTATE BIOPSY; PROSTATE CANCER; SENSITIVITY AND SPECIFICITY; SULFONATION; TISSUE CHARACTERIZATION;

EID: 67650504059     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.001     Document Type: Article

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