Preparation of genome-wide DNA fragment libraries using bisulfite in polyacrylamide gel electrophoresis slices with formamide denaturation and quality control for massively parallel sequencing by oligonucleotide ligation and detection

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 126-135

  Ranade, Swati S ^a   Chung, Christina Bormann ^a   Zon, Gerald ^a   Boyd, Victoria L ^a  

^a APPLIED BIOSYSTEMS   (United States)

Author keywords

Bisulfite; Capillary electrophoresis; DNA; Fragment library; Massively parallel sequencing by oligonucleotide ligation and detection; Polyacrylamide gel electrophoresis; Sanger sequencing; Single molecule PCR

Indexed keywords

ALKYLATION; AMIDES; CAPILLARY ELECTROPHORESIS; DNA; METHYLATION; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; QUALITY CONTROL;

BISULFITE; MASSIVELY PARALLEL SEQUENCING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SANGER SEQUENCING; SINGLE MOLECULE;

GENE ENCODING;

5 METHYLCYTOSINE; BISULFITE; CYTOSINE; DNA FRAGMENT; FORMAMIDE; THYMINE;

AMPLICON; ARTICLE; BIOINFORMATICS; CHROMOSOME MAP; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CPG ISLAND; DENATURATION; DNA LIBRARY; DNA METHYLATION; GENE AMPLIFICATION; GENE CONSTRUCT; GENOME; HIGH THROUGHPUT SCREENING; HUMAN; NUCLEOTIDE SEQUENCE; PARTICLE SIZE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITY CONTROL; RANDOMIZED CONTROLLED TRIAL; WEB BROWSER;

EID: 65849333933     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.020     Document Type: Article

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