Fluorescence complementation via EF-hand interactions

Journal of Biotechnology

Volumn 142, Issue 3-4, 2009, Pages 205-213

  Chen, Ning ^a   Ye, Yiming ^b   Zou, Jin ^a   Li, Shunyi ^a   Wang, Siming ^a   Martin, Amy ^b   Wohlhueter, Robert ^b   Yang, Jenny J ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

Calcium dependent; EF hand; EGFP; EGFP fluorescent fragment; Fluorescence complementation; Protein protein interactions

Indexed keywords

CALBINDIN; CALCIUM IONOPHORE; CLEAVAGE SITES; COMPLEMENTATION; EF-HAND; EF-HAND MOTIF; EGFP; EGFP FLUORESCENT FRAGMENT; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENCE ENHANCEMENT; FLUORESCENCE SIGNALS; FLUORESCENT FRAGMENTS; FLUORESCENT PROTEIN; GREEN FLUORESCENCE; HELA CELL; INTRACELLULAR CALCIUM; IONOMYCIN; LIVING CELL; NOVEL APPLICATIONS; PHYSIOLOGICAL CONDITION; PLASMID DNA; PROTEIN-PROTEIN INTERACTIONS; REASSEMBLY; SIGNALLING PATHWAYS; TARGET PROTEINS;

AMINES; AMINO ACIDS; CALCIUM; CALCIUM ALLOYS; CHROMOPHORES; MOLECULAR BIOLOGY; MOLECULAR RECOGNITION; NUCLEIC ACIDS; ORGANIC ACIDS; PROTEINS;

FLUORESCENCE;

CALBINDIN; CALCIUM; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT DYE; IONOMYCIN; PLASMID DNA; TRYPSIN;

AMINO ACID COMPOSITION; ARTICLE; CHROMATOPHORE; CONTROLLED STUDY; DIMERIZATION; FLUORESCENCE ANALYSIS; GENETIC COMPLEMENTATION; GENETIC TRANSFECTION; HELA CELL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; CALCIUM SIGNALING; CLONING, MOLECULAR; EF HAND MOTIFS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; KINETICS; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECOMBINANT FUSION PROTEINS; TRYPSIN;

EID: 67650064608     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.05.007     Document Type: Article

References (54)

1. Akemann W., Raj C.D., and Knopfel T. Functional characterization of permuted enhanced green fluorescent proteins comprising varying linker peptides. Photochem. Photobiol. 74 (2001) 356-363
2. Battistutta R., Negro A., and Zanotti G. Crystal structure and refolding properties of the mutant F99s/M153t/V163a of the green fluorescent protein. Proteins 41 (2000) 429-437
3. Belmont A.S. Visualizing chromosome dynamics with Gfp. Trends Cell Biol. 11 (2001) 250-257
4. Berggard T., Julenius K., Ogard A., Drakenberg T., and Linse S. Fragment complementation studies of protein stabilization by hydrophobic core residues. Biochemistry 40 (2001) 1257-1264
5. Brejc K., Sixma T.K., Kitts P.A., Kain S.R., Tsien R.Y., Ormo M., and Remington S.J. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 2306-2311
6. Cabantous S., and Waldo G.S. In vivo and in vitro protein solubility assays using split Gfp. Nat. Methods 3 (2006) 845-854
7. Chalfie M. Green fluorescent protein. Photochem. Photobiol. 62 (1995) 651-656
8. Chen J.J., Hong Y., Rustamzadeh E., Baleja J.D., and Androphy E.J. Identification of an alpha helical motif sufficient for association with Papillomavirus E6. J. Biol. Chem. 273 (1998) 13537-13544
9. Chiang C.F., Okou D.T., Griffin T.B., Verret C.R., and Williams M.N. Green fluorescent protein rendered susceptible to proteolysis: positions for protease-sensitive insertions. Arch. Biochem. Biophys. 394 (2001) 229-235
10. Cody C.W., Prasher D.C., Westler W.M., Prendergast F.G., and Ward W.W. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry 32 (1993) 1212-1218
11. Delbaere L.T., Vandonselaar M., and Reid R.E. Crystallization of a synthetic analog of calcium-binding site Iii of rabbit skeletal troponin C. J. Biol. Chem. 264 (1989) 10261-10263
12. Demidov V.V., Dokholyan N.V., Witte-Hoffmann C., Chalasani P., Yiu H.W., Ding F., Yu Y., Cantor C.R., and Broude N.E. Fast complementation of split fluorescent protein triggered by DNA hybridization. Proc. Natl. Acad. Sci. U.S.A. 103 (2006) 2052-2056
13. Dopf J., and Horiagon T.M. Deletion mapping of the Aequorea victoria green fluorescent protein. Gene 173 (1996) 39-44
14. Enoki S., Saeki K., Maki K., and Kuwajima K. Acid denaturation and refolding of green fluorescent protein. Biochemistry 43 (2004) 14238-14248
15. Flores-Ramirez G., Rivera M., Morales-Pablos A., Osuna J., Soberon X., and Gaytan P. The effect of amino acid deletions and substitutions in the longest loop of Gfp. BMC Chem. Biol. 7 (2007) 1
16. Heim R., Prasher D.C., and Tsien R.Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 12501-12504
17. Hu C.D., and Kerppola T.K. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat. Biotechnol. 21 (2003) 539-545
18. Hynes T.R., Tang L., Mervine S.M., Sabo J.L., Yost E.A., Devreotes P.N., and Berlot C.H. Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. J. Biol. Chem. 279 (2004) 30279-30286
19. Jaren O.R., Kranz J.K., Sorensen B.R., Wand A.J., and Shea M.A. Calcium-induced conformational switching of Paramecium calmodulin provides evidence for domain coupling. Biochemistry 41 (2002) 14158-14166
20. Jeong J., Kim S.K., Ahn J., Park K., Jeong E.J., Kim M., and Chung B.H. Monitoring of conformational change in maltose binding protein using split green fluorescent protein. Biochem. Biophys. Res. Commun. 339 (2006) 647-651
21. Julenius K., Robblee J., Thulin E., Finn B.E., Fairman R., and Linse S. Coupling of ligand binding and dimerization of helix-loop-helix peptides: spectroscopic and sedimentation analyses of calbindin D9k Ef-hands. Proteins 47 (2002) 323-333
22. Kay L.E., Forman-Kay J.D., McCubbin W.D., and Kay C.M. Solution structure of a polypeptide dimer comprising the fourth Ca(2+)-binding site of troponin C by nuclear magnetic resonance spectroscopy. Biochemistry 30 (1991) 4323-4333
23. Kim H.K., and Kaang B.K. Truncated green fluorescent protein mutants and their expression in Aplysia neurons. Brain Res. Bull. 47 (1998) 35-41
24. Li X., Zhang G., Ngo N., Zhao X., Kain S.R., and Huang C.C. Deletions of the Aequorea victoria green fluorescent protein define the minimal domain required for fluorescence. J. Biol. Chem. 272 (1997) 28545-28549
25. Linse S., and Chazin W.J. Quantitative measurements of the cooperativity in an Ef-hand protein with sequential calcium binding. Protein Sci. 4 (1995) 1038-1044
26. Lippincott-Schwartz J., and Patterson G.H. Development and use of fluorescent protein markers in living cells. Science 300 (2003) 87-91
27. Magliery T.J., Wilson C.G., Pan W., Mishler D., Ghosh I., Hamilton A.D., and Regan L. Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J. Am. Chem. Soc. 127 (2005) 146-157
28. 2+ based on green fluorescent proteins and calmodulin. Nature 388 (1997) 882-887
29. Mizuno T., Murao K., Tanabe Y., Oda M., and Tanaka T. Metal-ion-dependent Gfp emission in vivo by combining a circularly permutated green fluorescent protein with an engineered metal-ion-binding coiled-coil. J. Am. Chem. Soc. 129 (2007) 11378-11383
30. 2+. Proc. Natl. Acad. Sci. U.S.A. 98 (2001) 3197-3202
31. Niwa H., Inouye S., Hirano T., Matsuno T., Kojima S., Kubota M., Ohashi M., and Tsuji F.I. Chemical nature of the light emitter of the Aquorea green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 13617-13622
32. Ormo M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., and Remington S.J. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273 (1996) 1392-1395
33. Ozawa T., Nogami S., Sato M., Ohya Y., and Umezawa Y. A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. Anal. Chem. 72 (2000) 5151-5157
34. Periasamy A., and Day R.N. Visualizing protein interactions in living cells using digitized Gfp imaging and Fret microscopy. Methods Cell Biol. 58 (1999) 293-314
35. Perozzo M.A., Ward K.B., Thompson R.B., and Ward W.W. X-ray diffraction and time-resolved fluorescence analyses of Aequorea green fluorescent protein crystals. J. Biol. Chem. 263 (1988) 7713-7716
36. Prendergast F.G., and Mann K.G. Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskalea. Biochemistry 17 (1978) 3448-3453
37. Reid B.G., and Flynn G.C. Chromophore formation in green fluorescent protein. Biochemistry 36 (1997) 6786-6791
38. Revett S.P., King G., Shabanowitz J., Hunt D.F., Hartman K.L., Laue T.M., and Nelson D.J. Characterization of a helix-loop-helix (Ef hand) motif of silver hake Parvalbumin Isoform B. Protein Sci. 6 (1997) 2397-2408
39. Shaw G.S., Hodges R.S., and Sykes B.D. Calcium-induced peptide association to form an intact protein domain: 1 h Nmr structural evidence. Science 249 (1990) 280-283
40. Shimomura O. Structure of the chromophore of Aequorea green fluorescent protein. FEBS Lett. 104 (1979) 220-222
41. Shimomura O. The discovery of aequorin and green fluorescent protein. J. Microsc. 217 (2005) 1-15
42. Taniuchi H., Parr G.R., and Juillerat M.A. Complementation in folding and fragment exchange. Methods Enzymol. 131 (1986) 185-217
43. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67 (1998) 509-544
44. VanScyoc W.S., Newman R.A., Sorensen B.R., and Shea M.A. Calcium binding to calmodulin mutants having domain-specific effects on the regulation of ion channels. Biochemistry 45 (2006) 14311-14324
45. Ward W.W., and Bokman S.H. Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein. Biochemistry 21 (1982) 4535-4540
46. Wilson C.G., Magliery T.J., and Regan L. Detecting protein-protein interactions with Gfp-fragment reassembly. Nat. Methods 1 (2004) 255-262
47. Wood T.I., Barondeau D.P., Hitomi C., Kassmann C.J., Tainer J.A., and Getzoff E.D. Defining the role of Arginine 96 in green fluorescent protein fluorophore biosynthesis. Biochemistry 44 (2005) 16211-16220
48. Wouters F.S., Verveer P.J., and Bastiaens P.I. Imaging biochemistry inside cells. Trends Cell Biol. 11 (2001) 203-211
49. Yang F., Moss L.G., and Phillips Jr. G.N. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14 (1996) 1246-1251
50. Ye Y., Lee H.W., Yang W., Shealy S., and Yang J.J. Probing site-specific calmodulin calcium and lanthanide affinity by grafting. J. Am. Chem. Soc. 127 (2005) 3743-3750
51. Zhang J., Campbell R.E., Ting A.Y., and Tsien R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3 (2002) 906-918
52. Zhou Y., Yang W., Kirberger M., Lee H.W., Ayalasomayajula G., and Yang J.J. Prediction of Ef-hand calcium-binding proteins and analysis of bacterial Ef-hand proteins. Proteins 65 (2006) 643-655
53. Zimmer M. Green fluorescent protein (Gfp): applications, structure, and related photophysical behavior. Chem. Rev. 102 (2002) 759-781
54. 2+ concentrations. Biochemistry 46 (2007) 12275-12288