Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex

RNA

Volumn 15, Issue 7, 2009, Pages 1241-1250

  Schwer, Beate ^a   Schneider, Susanne ^a   Pei, Yi ^b   Aronova, Anna ^a   Shuman, Stewart ^b  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

Elongation checkpoint; mRNA capping; Transcription elongation

Indexed keywords

AMINO ACID; HETERODIMER; HYBRID PROTEIN; MESSENGER RNA PRECURSOR; PROTEIN SPT4; PROTEIN SPT5; RNA POLYMERASE II; SCHIZOSACCHAROMYCES POMBE PROTEIN; SERINE; TRYPSIN; UNCLASSIFIED DRUG; ZINC;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; FUNGAL GENE; FUNGUS GROWTH; GENETIC ANALYSIS; GENETIC COMPLEMENTATION; IN VIVO STUDY; MISSENSE MUTATION; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RNA PROCESSING; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SPT4 GENE; SPT5 GENE; TRANSCRIPTION REGULATION; TWO HYBRID SYSTEM;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CHROMOSOMAL PROTEINS, NON-HISTONE; DIMERIZATION; IMMUNOPRECIPITATION; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTIONAL ELONGATION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; SCHIZOSACCHAROMYCES POMBE;

EID: 67649373354     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.1572709     Document Type: Article

References (48)

1. Andrulis ED, Guzman E, Döring P, Werner J, Lis JT. 2000. High-resolution localization of Drosophila Spt5 Spt6 at heat shock genes in vivo: Roles in promoter proximal pausing and transcription elongation. Genes & Dev 14: 2635-2649. (Pubitemid 30786715)
2. Basrai MA, Kingsbury J, Koshland D, Spencer F, Hieter P. 1996. Faithful chromosome transmission requires Spt4p, a putative regulator of chromatin structure in Saccharomyces cerevisiae. Mol Cell Biol 16: 2838-2847. (Pubitemid 26161288)
3. Chiu YL, Ho CK, Saha N, Schwer B, Shuman S, Rana TM. 2002. Tat stimulates cotranscriptional capping of HIV mRNA. Mol Cell 10: 585-597. (Pubitemid 35284175)
4. Cho E, Takagi T, Moore CR, Buratowski S. 1997. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxyl-terminal domain. Genes & Dev 11: 3319-3326. (Pubitemid 28023956)
5. Fabrega C, Shen V, Shuman S, Lima CD. 2003. Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II. Mol Cell 11: 1549-1561. (Pubitemid 36776541)
6. Forsburg SL. 1993. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res 21: 2955-2956. (Pubitemid 23220463)
7. Gao L, Gross DS. 2008. Sir2 silences gene transcription by targeting the transition between RNA polymerase II initiation and elongation. Mol Cell Biol 28: 3979-3994. (Pubitemid 351812984)
8. Goldstein AL, McCusker JH. 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553. (Pubitemid 29472165)
9. Guiguen A, Soutourine J, Dewez M, Tafforeau L, Dieu M, Raes M, Vandenhaute J, Werner M, Hermand D. 2007. Recruitment of P-TEFb (Cdk9-Pch1) to chromatin by the cap-methyl transferase Pcm1 in fission yeast. EMBO J 26: 1552-1559. (Pubitemid 46480946)
10. Güldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524. (Pubitemid 26237149)
11. Guo S, Yamaguchi Y, Schilbach S, Wada T, Lee J, Goddard A, French D, Handa H, Rosenthal A. 2000. A regulator of transcription elongation controls vertebrate neuronal development. Nature 408: 366-369.
12. Guo M, Xu F, Yamada J, Egelhofer T, Gao Y, Kartzog GA, Teng M, Niu L. 2008. Core structure of the yeast Spt4-Spt5 complex: A conserved module for regulation of transcription elongation. Structure 16: 1649-1658.
13. Guthrie C, Fink G. 1991. Guide to yeast genetics and molecular biology. Academic Press, San Diego, CA.
14. Hartzog GA, Basrai MA, Ricupero-Hovasse SL, Hieter P, Winston F. 1996. Identification and analysis of a functional human homolog of the SPT4 gene of Saccharomyces cerevisiae. Mol Cell Biol 16: 2848-2856. (Pubitemid 26161289)
15. Hartzog GA, Wada T, Handa H, Winston F. 1998. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes & Dev 12: 357-369. (Pubitemid 28084264)
16. Hikkel I, Gbelska Y, Subik J. 1998. Identification and functional analysis of a Kluyveromyces lactis homologue of the SPT4 gene of Saccharomyces cerevisiae. Curr Genet 34: 375-378. (Pubitemid 29004642)
17. Ho CK, Shuman S. 1999. Distinct roles for CTD Ser2 and Ser5 phosphorylation in the recruitment and allosteric activation of mammalian capping enzyme. Mol Cell 3: 405-411. (Pubitemid 29290681)
18. Ivanov D, Kwak YT, Guo J, Gaynor RB. 2000. Domains in the Spt5 protein that modulate its transcriptional regulatory properties. Mol Cell Biol 20: 2970-2983. (Pubitemid 30215010)
19. Jennings BH, Shah S, Yamaguchi Y, Seki M, Phillips RG, Handa H, Ish-Horowicz D. 2004. Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila. Curr Biol 14: 1680-1684. (Pubitemid 39239367)
20. Kim D, Inukai N, Yamada T, Furuya A, Sata H, Yamaguchi Y, Wada T, Handa H. 2003. Structure-function analysis of human Spt4: Evidence that hSpt4 and hSp5 exert their roles in transcriptional elongation as parts of the DSIF complex. Genes Cells 8: 371-378. (Pubitemid 36504012)
21. Lehman K, Schwer B, Ho CK, Rouzankina I, Shuman S. 1999. A conserved domain of yeast RNA triphosphatase flanking the catalytic core regulates self-association and interaction with the guanylyltransferase component of the mRNA capping apparatus. J Biol Chem 274: 22668-22678. (Pubitemid 129529288)
22. Lindstrom DL, Squazzo SL, Mister N, Burckin TA, Wachter KC, Emigh CA, McCleery JA, Yates JR, Hartzog GA. 2003. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23: 1368-1378. (Pubitemid 36177045)
23. Malone EA, Fassler JS, Winston F. 1993. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae. Mol Gen Genet 237: 449-459. (Pubitemid 23080518)
24. Mandal SS, Chu C, Wada T, Handa H, Shatkin AJ, Reinberg D. 2004. Functional interaction of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II. Proc Natl Acad Sci 101: 7572-7577. (Pubitemid 38658070)
25. Mason PB, Struhl K. 2005. Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo. Mol Cell 17: 831-840. (Pubitemid 40386944)
26. McCracken S, Fong N, Rosonina E, Yankulov K, Brothers G, Siderovski D, Hessel A, Foster S, Shuman S, Bentley DL. 1997. 5′ Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated C-terminal domain of RNA polymerase II. Genes & Dev 11: 3306-3318. (Pubitemid 28023955)
27. Pei Y, Shuman S. 2002. Interactions between fission yeast mRNA capping enzymes and elongation factor Spt5. J Biol Chem 277: 19639-19648. (Pubitemid 34967478)
28. Pei Y, Shuman S. 2003. Characterization of the Schizosaccharomyces pombe Cdk9/Pch1 protein kinase: Spt5 phosphorylation, autophosphorylation and mutational analysis. J Biol Chem 278: 43346-43356. (Pubitemid 37345956)
29. Pei Y, Hausmann S, Ho CK, Schwer B, Shuman S. 2001. The length, phosphorylation state, and primary structure of the RNA polymerase II carboxyl-terminal domain dictate interactions with mRNA capping enzymes. J Biol Chem 276: 28075-28082. (Pubitemid 37391507)
30. Pei Y, Schwer B, Shuman S. 2003. Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control. J Biol Chem 278: 7180-7188. (Pubitemid 36800717)
31. Pei Y, Du H, Singer J, St. Amour C, Granitto S, Shuman S, Fisher RP. 2006. Cdk9 of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the cap methyltransferase Pcm1 in vivo. Mol Cell Biol 26: 777-788. (Pubitemid 43146475)
32. Ponting CP. 2002. Novel domains and orthologues of eukaryotic transcription elongation factors. Nucleic Acids Res 30: 3643-3652. (Pubitemid 35021228)
33. Qui H, Hu C, Wong CM, Hinnebusch AG. 2006. The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II. Mol Cell Biol 26: 3135-3148.
34. Renner DB, Yamaguchi Y, Wada T, Handa H, Price DH. 2001. A highly purified RNA polymerase II elongation control system. J Biol Chem 276: 42601-42609. (Pubitemid 37371176)
35. Rondón AG, García-Rubio M, González-Barrera S, Aguilera A. 2003. Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22: 612-620. (Pubitemid 36193605)
36. Schiestl RH, Gietz RD. 1989. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet 16: 339-346. (Pubitemid 20034019)
37. Schneider DA, French SL, Osheim YN, Bailey AO, Lu V, Dodd J, Yates JR, Beyer AL, Nomura M. 2006. RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci 103: 12707-12712. (Pubitemid 44298625)
38. Shim EY, Walker AK, Shi Y, Blackwell KT. 2002. CDK-9/cyclin T (PTEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes & Dev 16: 2135-2146. (Pubitemid 34898932)
39. Sims RJ, Belotserkovskaya R, Reinberg D. 2004. Elongation by RNA polymerase II: The short and long of it. Genes & Dev 18: 2437-2468. (Pubitemid 39362887)
40. Swanson MS, Malone EA, Winston F. 1991. SPT5, and essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat. Mol Cell Biol 11: 3009-3019. (Pubitemid 21895686)
41. Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, et al. 1998a. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes & Dev 12: 343-356. (Pubitemid 28084263)
42. Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H. 1998b. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro. EMBO J 17: 7395-7403. (Pubitemid 29002707)
43. Wen Y, Shatkin AJ. 1999. Transcription elongation factor hSpt5 stimulates mRNA capping. Genes & Dev 13: 1774-1779. (Pubitemid 29353045)
44. Wenzel S, Schweimer K, Rösch P, Wöhlri BM. 2008. The small hSpt4 subnit of the human transcription elongation factor DSIF is a Zn-finger protein with α/β-type topology. Biochem Biophys Res Commun 370: 414-418.
45. Wu CH, Yamaguchi Y, Benjamin LR, Horvat-Gordon M, Washinky J, Enerly E, Larsson J, Lambertsson A, Handa H, Gilmour D. 2003. NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes & Dev 17: 1402-1414. (Pubitemid 36666009)
46. Yamada T, Yamaguchi Y, Inukai N, Okamoto S, Mura T, Handa H. 2006. P-TEFb-mediated phosphorylation of hSpt5 C-terminal repeats is critical for processive transcription elongation. Mol Cell 21: 227-237. (Pubitemid 43099938)
47. Yamaguchi Y, Wada T, Watanabe D, Takagi T, Hasegawa J, Handa H. 1999. Structure and function of the human transcription elongation factor DSIF. J Biol Chem 274: 8085-8092. (Pubitemid 129518205)
48. Yue Z, Maldonado E, Pillutla R, Cho H, Reinberg D, Shatkin AJ. 1997. Mammalian capping enzyme complements mutant S. cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II. Proc Natl Acad Sci 94: 12898-12903. (Pubitemid 27518448)