Measurement of the unstained biological sample by a novel scanning electron generation X-ray microscope based on SEM

Biochemical and Biophysical Research Communications

Volumn 385, Issue 4, 2009, Pages 624-629

  Ogura, Toshihiko ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Biological sample; Indirect secondary electron contrast; Monte Carlo simulation; Scanning electron microscopy; Silicon nitride film; Titanium coating; X ray microscopy

Indexed keywords

SILICON NITRIDE; TITANIUM;

ADSORPTION; ARTICLE; CONTROLLED STUDY; ELECTRON BEAM; FILM; MONTE CARLO METHOD; NONHUMAN; PRIORITY JOURNAL; ROENTGEN MICROSCOPY; SACCHAROMYCES CEREVISIAE; SCANNING ELECTRON MICROSCOPY;

MICROSCOPY, ELECTRON, SCANNING; MONTE CARLO METHOD; SACCHAROMYCES CEREVISIAE; SILICON COMPOUNDS; TITANIUM; X-RAY FILM; X-RAYS;

EID: 67349193233     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.05.107     Document Type: Article

References (22)

1. Kirz J., Jacobsen C., and Howells M. Soft X-ray microscopes and their biological applications. Q. Rev. Biophys. 28 (1995) 33-130
2. Jacobsen C., Kirz J., and Williams S. Resolution in soft X-ray microscopes. Ultramicroscopy 47 (1992) 55-79
3. Jacobsen C. Soft X-ray microscopy. Trends Cell Biol. 9 (1999) 44-47
4. Sayre D., Kirz J., Feder R., Kim D.M., and Spiller E. Transmission microscopy of unmodified biological materials: comparative radiation dosages with electrons and ultrasoft X-ray photons. Ultramicroscopy 2 (1977) 337-349
5. Chao W., Harteneck B.D., Liddle J.A., Anderson E.H., and Attwood D.T. Soft X-ray microscopy at a spatial resolution better than 15 nm. Nature 435 (2005) 1210-1213
6. Schneider G. Cryo X-ray microscopy with high spatial resolution in amplitude and phase contrast. Ultramicroscopy 75 (1998) 85-104
7. Larabell C.A., and Le Gros M.A. X-ray tomography generates 3-D reconstructions of the yeast, Sacharomyces cerevisiae, at 60-nm resolution. Mol. Biol. Cell 15 (2004) 957-962
8. Williams S., Jacobsen C., Kirz J., Maser J., Wirick S., Zhang X., Ade H., and Rivers M. Instrumentation developments in scanning soft X-ray microscopy at the NSLS. Rev. Sci. Instrum. 66 (1995) 1271-1275
9. Zhang X., Balhorn R., Mazrimas J., and Kirz J. Mapping and measuring DNA to protein ratios in mammalian sperm head by XANES imaging. J. Struct. Biol. 116 (1996) 335-344
10. Miao J., Charalambous P., Kirz J., and Sayre D. Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens. Nature 400 (1999) 342-344
11. Shapiro D., Thibault P., Beetz T., Elser V., Howells M., Jacobsen C., Kirz J., Lima E., Miao H., Neiman A.M., and Sayre D. Biological imaging by soft X-ray diffraction microscopy. Proc. Natl. Acad. Sci. USA 102 (2005) 15343-15346
12. Song C., Jiang H., Mancuso A., Amirbekian B., Peng L., Sun R., Shah S.S., Zhou Z.H., Ishikawa T., and Miao J. Quantitative imaging of single, unstained viruses with coherent X rays. Phys. Rev. Lett. 101 (2008) 158101
13. Mayo S.C., Davis T.J., Gureyev T.E., Miller P.R., Paganin D., Pogany A., Stevenson A.W., and Wilkins S.W. X-ray phase-contrast microscopy and microtomography. Opt. Express 11 (2003) 2289-2302
14. Tomie T., Shimizu H., Majima T., Yamada M., Kanayama T., Kondo H., Yano M., and Ono M. Three-dimensional readout of flash X-ray images of living sperm in water by atomic-force microscopy. Science 252 (1991) 691-693
15. Ogura T. A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy. Biochem. Biophys. Res. Commun. 377 (2008) 79-84
16. Ogura T. Analyzing indirect secondary electron contrast of unstained bacteriophage T4 based on SEM images and Monte Carlo simulations. Biochem. Biophys. Res. Commun. 380 (2009) 254-259
17. Gullikson E.M., Korde R., Canfield L.R., and Vest R.E. Stable silicon photodiodes for absolute intensity measurements in the VUV and soft X-ray regions. J. Electron Spectrosc. Relat. Phenom. 80 (1996) 313-316
18. Drouin D., Couture A.R., Joly D., Tastet X., Aimez V., and Gauvin R. CASINO V2.42 - a fast and easy-to-use modeling tool for scanning electron microscopy and microanalysis users. Scanning 29 (2007) 92-101
19. 3 by low pressure chemical vapor deposition: kinetics, thermodynamics, composition and structure. Thin Solid Films 213 (1992) 182-191
20. Funsten H.O., Suszcynsky D.M., Ritzau S.M., and Korde R. Response of 100% internal quantum efficiency silicon photodiodes to 200 eV-40 keV electrons. IEEE Trans. Nucl. Sci. 44 (1997) 2561-2565
21. Grella L., Lorusso G., Niemi T., and Adler D.L. Simulations of SEM imaging and charging. Nucl. Instrum. Methods Phys. Res. A 519 (2004) 242-250
22. Henke B.L., Gullikson E.M., and Davis J.C. X-ray interactions: photoabsorption, scattering, transmission, and reflection at E = 50-30,000 eV, Z = 1-92. Atom. Data Nucl. Data Tables 54 (1993) 181-342