LNA flow-FISH: A flow cytometry-fluorescence in situ hybridization method to detect messenger RNA using locked nucleic acid probes

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 109-114

  Robertson, Kelly L ^a   Thach, Dzung C ^b  

^a NAVAL RESEARCH LABORATORY   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Flow cytometry; Fluorescence in situ hybridization (FISH); Locked nucleic acid (LNA); messenger RNA (mRNA) detection; Actin

Indexed keywords

AMIDES; FISH; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; OLIGONUCLEOTIDES; PROBES; PROTEINS;

DETECTION AND QUANTIFICATIONS; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE SIGNALS; LOCKED NUCLEIC ACID; MESSENGER RNAS (MRNA); MODIFIED OLIGONUCLEOTIDES; OPTIMAL CONDITIONS; PERMEABILIZATION;

RNA;

BETA ACTIN; BUFFER; FORMAMIDE; LOCKED NUCLEIC ACID; MESSENGER RNA; PHYCOERYTHRIN; PROTEINASE K; STREPTAVIDIN; TELOMERASE;

ARTICLE; BIOTINYLATION; CELL PERMEABILIZATION; CELL SUSPENSION; CONTROLLED STUDY; ENZYME ANALYSIS; EPITHELIUM CELL; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE IDENTIFICATION; GENE TARGETING; HUMAN; HUMAN CELL; NUCLEIC ACID PROBE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; PROTEIN BINDING; RNA BINDING; SIGNAL TRANSDUCTION; STAINING; TEMPERATURE;

EID: 67349155946     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.026     Document Type: Article

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