Efficient sequential gene regulation via FLP- and Cre-recombinase using adenovirus vector in mammalian cells including mouse ES cells

Microbiology and Immunology

Volumn 50, Issue 10, 2006, Pages 831-843

  Kondo, Saki ^a   Takahashi, Yuzuka ^a   Shiozawa, Seiji ^a   Ichise, Hirotake ^a   Yoshida, Nobuaki ^a   Kanegae, Yumi ^a   Saito, Izumu ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Adenovirus vector; Cre loxP system; FLP FRT system; Gene regulation

Indexed keywords

CRE RECOMBINASE; RECOMBINASE;

ADENOVIRUS VECTOR; ANIMAL CELL; ARTICLE; CHROMOSOME ANALYSIS; CONTROLLED STUDY; CYTOTOXICITY; EMBRYONIC STEM CELL; GENE CASSETTE; GENE CONSTRUCT; GENE CONTROL; GENE SEQUENCE; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; QUALITATIVE ANALYSIS; SOUTHERN BLOTTING; VIRUS INFECTION;

ADENOVIRIDAE; MAMMALIA;

EID: 33750360812     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2006.tb03850.x     Document Type: Article

References (47)

1. Aladjem, M.I., Rodewald, L.W., Kolman, J.L., and Wahl, G.M. 1998. Genetic dissection of a mammalian replicator in the human beta-globin locus. Science 281: 1005-1009.
2. Andrews, B.J., Proteau, G.A., Beatty, L.G., and Sadowski, P.D. 1985. The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences. Cell 40: 795-803.
3. Anton, M., and Graham, F.L. 1995. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J. Virol. 69: 4600-4606.
4. Baba, Y., Nakano, M., Yamada, Y., Saito, I., and Kanegae, Y. 2005. Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. Microbiol. Immunol. 49: 559-570.
5. Barinaga, M. 1994. Knockout mice: round two. Science 265: 26-28.
6. Broach, J.R., Guarascio, V.R., and Jayaram, M. 1982. Recombination within the yeast plasmid 2mu circle is site-specific. Cell 29: 227-234.
7. Buchholz, F., Angrand, P.O., and Stewart, A.F. 1998. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat. Biotechnol. 16: 657-662.
8. DeMayo, F.J., and Tsai, S.Y. 2001. Targeted gene regulation and gene ablation. Trends Endocrinol. Metab. 12: 348-353.
9. Fukushige, S., and Sauer, B. 1992. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 89: 7905-7909.
10. Gossen, M., and Bujard, H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. U.S.A. 89: 5547-5551.
11. Gossen, M., Freundlieb, S., Bender, G., Muller, G., Hillen, W., and Bujard, H. 1995. Transcriptional activation by tetracyclines in mammalian cells. Science 268: 1766-1769.
12. Graham, F.L., Smiley, J., Russell, W.C., and Nairn, R. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-74.
13. Gu, H., Zou, Y.R., and Rajewsky, K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 73: 1155-1164.
14. Jayaram, M. 1997. The cis-trans paradox of integrase. Science 276: 49-51.
15. Kaartinen, V., and Nagy, A. 2001. Removal of the floxed neo gene from a conditional knockout allele by the adenoviral Cre recombinase in vivo. Genesis 31: 126-129.
16. Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., and Saito, I. 1995. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23: 3816-3821.
17. Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., and Saito, I. 1996. Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene 181: 207-212.
18. Kawabata, K., Sakurai, F., Yamaguchi, T., Hayakawa, T., and Mizuguchi, H. 2005. Efficient gene transfer into mouse embryonic stem cells with adenovirus vectors. Mol. Ther. 12: 547-554.
19. Kim, D.W., Uetsuki, T., Kaziro, Y., Yamaguchi, N., and Sugano, S. 1990. Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene 91: 217-223.
20. Kondo, S., Okuda, A., Sato, H., Tachikawa, N., Terashima, M., Kanegae, Y., and Saito, I. 2003. Simultaneous on/off regulation of transgenes located on a mammalian chromosome with Cre-expressing adenovirus and a mutant loxP. Nucleic Acids Res. 31: e76.
21. Lakso, M., Pichel, J.G., Gorman, J.R., Sauer, B., Okamoto, Y., Lee, E., Alt, F.W., and Westphal, H. 1996. Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc. Natl. Acad. Sci. U.S.A. 93: 5860-5865.
22. Lakso, M., Sauer, B., Mosinger, B., Jr., Lee, E.J., Manning, R.W., Yu, S.H., Mulder, K.L., and Westphal, H. 1992. Targeted oncogene activation by site-specific recombination in transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 89: 6232-6236.
23. Lewandoski, M. 2001. Conditional control of gene expression in the mouse. Nat. Rev. Genet. 2: 743-755.
24. Li, X.K., Fujino, M., Guo, L., Okuyama, T., Funeshima, N., Hashimoto, M., Okabe, K., Yaginuma, H., Mikoshiba, K., Enosawa, S., Amemiya, H., and Suzuki, S. 2000. Inhibition of Fas-mediated fulminant hepatitis in CrmA gene-transfected mice. Biochem. Biophys. Res. Commun. 273: 101-109.
25. Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., and Saito, I. 1996. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. U.S.A. 93: 1320-1324.
26. Nakano, M., Odaka, K., Ishimura, M., Kondo, S., Tachikawa, N., Chiba, J., Kanegae, Y., and Saito, I. 2001. Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus. Nucleic Acids Res. 29: E40.
27. Niwa, H., Yamamura, K., and Miyazaki, J. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193-199.
28. O'Gorman, S., Fox, D.T., and Wahl, G.M. 1991. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251: 1351-1355.
29. Okuyama, T., Fujino, M., Li, X.K., Funeshima, N., Kosuga, M., Saito, I., Suzuki, S., and Yamada, M. 1998. Efficient Fas-ligand gene expression in rodent liver after intravenous injection of a recombinant adenovirus by the use of a Cre-mediated switching system. Gene Ther. 5: 1047-1053.
30. Pfeifer, A., Brandon, E.P., Kootstra, N., Gage, F.H., and Verma, I.M. 2001. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Proc. Natl. Acad. Sci. U.S.A. 98: 11450-11455.
31. Rohlmann, A., Gotthardt, M., Willnow, T.E., Hammer, R.E., and Herz, J. 1996. Sustained somatic gene inactivation by viral transfer of Cre recombinase. Nat. Biotechnol. 14: 1562-1565.
32. Saito, I., Groves, R., Giulotto, E., Rolfe, M., and Stark, G.R. 1989. Evolution and stability of chromosomal DNA coamplified with the CAD gene. Mol. Cell. Biol. 9: 2445-2452.
33. Saito, I., Oya, Y., Yamamoto, K., Yuasa, T., and Shimojo, H. 1985. Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome. J. Virol. 54: 711-719.
34. Sambrook, J., and Russell, D.W. 2001. Molecular cloning, 3rd ed, Cold Spring Harbor Laboratory, New York.
35. Sauer, B., and Henderson, N. 1988. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl. Acad. Sci. U.S.A. 85: 5166-5170.
36. Schaft, J., Ashery-Padan, R., van der Hoeven, F., Gruss, P., and Stewart, A.F. 2001. Efficient FLP recombination in mouse ES cells and oocytes. Genesis 31: 6-10.
37. Senecoff, J.F., Bruckner, R.C., and Cox, M.M. 1985. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site. Proc. Natl. Acad. Sci. U.S. A. 82: 7270-7274.
38. Shibata, H., Toyama, K., Shioya, H., Ito, M., Hirota, M., Hasegawa,, S., Matsumoto, H., Takano, H., Akiyama, T., Toyoshima, K., Kanamaru, R., Kanegae, Y., Saito, I., Nakamura, Y., Shiba, K., and Noda, T. 1997. Rapid colorectal adenoma formation initiated by conditional targeting of the Ape gene. Science 278: 120-123.
39. Shintani, Y., Yotsuyanagi, H., Moriya, K., Fujie, H., Tsutsumi, T., Kanegae, Y., Kimura, S., Saito, I., and Koike, K. 1999. Induction of apoptosis after switch-on of the hepatitis B virus X gene mediated by the Cre/loxP recombination system. J. Gen. Virol. 80 (Pt 12): 3257-3265.
40. Shui, J.W., and Tan, T.H. 2004. Germline transmission and efficient DNA recombination in mouse embryonic stem cells mediated by adenoviral-Cre transduction. Genesis 39: 217-223.
41. Silver, D.P., and Livingston, D.M. 2001. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol. Cell 8: 233-243.
42. Sternberg, N., and Hamilton, D. 1981. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J. Mol. Biol. 150: 467-486.
43. van der Neut, R. 1997. Targeted gene disruption: applications in neurobiology. J. Neurosci. Methods 71: 19-27.
44. Wakita, T., Taya, C., Katsume, A., Kato, J., Yonekawa, H., Kanegae, Y., Saito, I., Hayashi, Y., Koike, M., and Kohara, M. 1998. Efficient conditional transgene expression in hepatitis C virus cDNA transgenic mice mediated by the Cre/loxP system. J. Biol. Chem. 273: 9001-9006.
45. Xu, X., Li, C., Garrett-Beal, L., Larson, D., Wynshaw-Boris, A., and Deng, C.X. 2001. Direct removal in the mouse of a floxed neo gene from a three-loxP conditional knockout allele by two novel approaches. Genesis 30: 1-6.
46. Zhang, H.G., Bilbao, G., Zhou, T., Contreras, J.L., Gomez-Navarro, J., Feng, M., Saito, I., Mountz, J.D., and Curiel, D.T. 1998. Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression. J. Virol. 72: 2483-2490.
47. Zhu, Z., Zheng, T., Lee, C.G., Homer, R.J., and Elias, J.A. 2002. Tetracycline-controlled transcriptional regulation systems: advances and application in transgenic animal modeling. Semin. Cell Dev. Biol. 13: 121-128.