Positive selection of FLP-mediated unequal sister chromatid exchange products in mammalian cells

Molecular and Cellular Biology

Volumn 17, Issue 2, 1997, Pages 857-861

  Aladjem, Mirit I ^a   Brody, Linnea L ^a   O'Gorman, Stephen ^a   Wahl, Geoffrey M ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLASTICIDIN S; RECOMBINASE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; CHROMOSOME STRUCTURE; EXCISION REPAIR; GENE DOSAGE; GENE FUNCTION; GENETIC RECOMBINATION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; SEQUENCE ANALYSIS; SISTER CHROMATID EXCHANGE;

EID: 0031053576     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.2.857     Document Type: Article

References (14)

1. Dale, E. C., and D. W. Ow. 1991. Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88: 10558-10562.
2. Fukushige, S., and B. Sauer. 1992. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 89:7905-7909.
3. Golic, K. 1991. Site-specific recombination between homologous chromosomes in Drosophila. Science 252:958-961.
4. Golic, K. G. 1994. Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase. Genetics 137:551-563.
5. Gu, H., J. D. Marth, P. C. Orban, H. Mossmann, and K. Rajewsky. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
6. Izumi, M., H. Miyazawa, T. Kamakura, I. Yamaguchi, T. Endo, and F. Hanaoka. 1991. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp. Cell Res. 197:229-233.
7. Kelly, R. E., and G. M. Wahl. Unpublished data.
8. Lloyd, A. M., and R. W. Davis. 1994. Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol. Gen. Genet. 242:653-657.
9. Nolan, G. P., S. Fiering, A. Nicolas, and L. A. Herzenberg. 1988. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proc. Natl. Acad. Sci. USA 85:2603-2607.
10. O'Gorman, S., D. T. Fox, and G. M. Wahl. 1991. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251:1351-1355.
11. Ramirez-Solis, R., P. Liu, and A. Bradley. 1995. Chromosome engineering in mice. Nature 378:720-724.
12. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
13. Shimizu, N., and G. M. Wahl. 1996. Micronucleation provides a rapid method for purifying amplified DNA lacking functional centromere. Nat. Genet. 12:65-71.
14. Smith, A. J., S. M. De, A. B. Kwabi, P. A. Heppell, H. Impey, and P. Rabbitts. 1995. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nat. Genet. 9:376-385.