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Volumn 4, Issue 6, 2009, Pages 928-938

Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; CELL PROTEIN; MESSENGER RNA; PROTEIN COL2A1; UNCLASSIFIED DRUG;

EID: 66749129094     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.63     Document Type: Article
Times cited : (47)

References (50)
  • 1
    • 0034914546 scopus 로고    scopus 로고
    • The effects of static and intermittent compression on nitric oxide production in articular cartilage explants
    • Fermor, B. et al. The effects of static and intermittent compression on nitric oxide production in articular cartilage explants. J. Orthop. Res. 19, 729-737 (2001).
    • (2001) J. Orthop. Res , vol.19 , pp. 729-737
    • Fermor, B.1
  • 2
    • 70350613981 scopus 로고    scopus 로고
    • Prostaglandin E2 synthesis in cartilage explants under compression: MPGES-1 is a mechanosensitive gene
    • Gosset, M. et al. Prostaglandin E2 synthesis in cartilage explants under compression: MPGES-1 is a mechanosensitive gene. Arthritis Res. Ther. 8, R135 (2006).
    • (2006) Arthritis Res. Ther , vol.8
    • Gosset, M.1
  • 3
    • 33646184317 scopus 로고    scopus 로고
    • Mechanical compressive loading stimulates the activity of proximal region of human COL2A1 gene promoter in transfected chondrocytes
    • Xie, J., Han, Z.Y. & Matsuda, T. Mechanical compressive loading stimulates the activity of proximal region of human COL2A1 gene promoter in transfected chondrocytes. Biochem. Biophys. Res. Commun. 344, 1192-1199 (2006).
    • (2006) Biochem. Biophys. Res. Commun , vol.344 , pp. 1192-1199
    • Xie, J.1    Han, Z.Y.2    Matsuda, T.3
  • 4
    • 0031890531 scopus 로고    scopus 로고
    • Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants
    • Quinn, T.M. et al. Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants. J. Cell Sci. 111 (Part 5): 573-583 (1998).
    • (1998) J. Cell Sci , vol.111 , Issue.PART 5 , pp. 573-583
    • Quinn, T.M.1
  • 5
    • 2442531856 scopus 로고    scopus 로고
    • Mechanical compression of cartilage explants induces multiple time-dependent gene expression patterns and involves intracellular calcium and cyclic AMP
    • Fitzgerald, J.B. et al. Mechanical compression of cartilage explants induces multiple time-dependent gene expression patterns and involves intracellular calcium and cyclic AMP. J. Biol. Chem. 279, 19502-19511 (2004).
    • (2004) J. Biol. Chem , vol.279 , pp. 19502-19511
    • Fitzgerald, J.B.1
  • 6
    • 0024730015 scopus 로고
    • Biosynthetic response of cartilage explants to dynamic compression
    • Sah, R.L. et al. Biosynthetic response of cartilage explants to dynamic compression. J. Orthop. Res. 7, 619-636 (1989).
    • (1989) J. Orthop. Res , vol.7 , pp. 619-636
    • Sah, R.L.1
  • 7
    • 0026928502 scopus 로고
    • Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro
    • Parkkinen, J.J., Lammi, M.J., Helminen, H.J. & Tammi, M. Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro. J. Orthop. Res. 10, 610-620 (1992).
    • (1992) J. Orthop. Res , vol.10 , pp. 610-620
    • Parkkinen, J.J.1    Lammi, M.J.2    Helminen, H.J.3    Tammi, M.4
  • 8
    • 0037766027 scopus 로고    scopus 로고
    • Proteoglycan metabolism and viability of articular cartilage explants as modulated by the frequency of intermittent loading
    • Sauerland, K., Raiss, R.X. & Steinmeyer, J. Proteoglycan metabolism and viability of articular cartilage explants as modulated by the frequency of intermittent loading. Osteoarthr. Cartil. 11, 343-350 (2003).
    • (2003) Osteoarthr. Cartil , vol.11 , pp. 343-350
    • Sauerland, K.1    Raiss, R.X.2    Steinmeyer, J.3
  • 9
    • 33847611088 scopus 로고    scopus 로고
    • Cyclic compression loaded on cartilage explants enhances the production of reactive oxygen species
    • Tomiyama, T. et al. Cyclic compression loaded on cartilage explants enhances the production of reactive oxygen species. J. Rheumatol. 34, 556-562 (2007).
    • (2007) J. Rheumatol , vol.34 , pp. 556-562
    • Tomiyama, T.1
  • 10
    • 0032810715 scopus 로고    scopus 로고
    • Cyclic compression of articular cartilage explants is associated with progressive consolidation and altered expression pattern of extracellular matrix proteins
    • Wong, M., Siegrist, M. & Cao, X. Cyclic compression of articular cartilage explants is associated with progressive consolidation and altered expression pattern of extracellular matrix proteins. Matrix Biol. 18, 391-399 (1999).
    • (1999) Matrix Biol , vol.18 , pp. 391-399
    • Wong, M.1    Siegrist, M.2    Cao, X.3
  • 11
    • 0028969166 scopus 로고
    • Mechanical compression modulates matrix biosynthesis in chondrocyte/ agarose culture
    • Buschmann, M.D., Gluzband, Y.A., Grodzinsky, A.J. & Hunziker, E.B. Mechanical compression modulates matrix biosynthesis in chondrocyte/ agarose culture. J. Cell Sci. 108 (Part 4): 1497-1508 (1995).
    • (1995) J. Cell Sci , vol.108 , Issue.PART 4 , pp. 1497-1508
    • Buschmann, M.D.1    Gluzband, Y.A.2    Grodzinsky, A.J.3    Hunziker, E.B.4
  • 12
    • 33747117890 scopus 로고    scopus 로고
    • Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures
    • Kelly, T.A. et al. Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J. Biomech. 39, 1489-1497 (2006).
    • (2006) J. Biomech , vol.39 , pp. 1489-1497
    • Kelly, T.A.1
  • 13
    • 0142103961 scopus 로고    scopus 로고
    • Hydrostatic pressure modulates proteoglycan metabolism in chondrocytes seeded in agarose
    • Toyoda, T. et al. Hydrostatic pressure modulates proteoglycan metabolism in chondrocytes seeded in agarose. Arthritis Rheum. 48, 2865-2872 (2003).
    • (2003) Arthritis Rheum , vol.48 , pp. 2865-2872
    • Toyoda, T.1
  • 14
    • 0042360401 scopus 로고    scopus 로고
    • Synergistic action of growth factors and dynamic loading for articular cartilage tissue engineering
    • Mauck, R.L. et al. Synergistic action of growth factors and dynamic loading for articular cartilage tissue engineering. Tissue Eng. 9, 597-611 (2003).
    • (2003) Tissue Eng , vol.9 , pp. 597-611
    • Mauck, R.L.1
  • 15
    • 0043063935 scopus 로고    scopus 로고
    • Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression
    • Chowdhury, T.T., Bader, D.L., Shelton, J.C. & Lee, D.A. Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression. Arch. Biochem. Biophys. 417, 105-111 (2003).
    • (2003) Arch. Biochem. Biophys , vol.417 , pp. 105-111
    • Chowdhury, T.T.1    Bader, D.L.2    Shelton, J.C.3    Lee, D.A.4
  • 16
    • 0020369710 scopus 로고
    • Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
    • Benya, P.D. & Shaffer, J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30, 215-224 (1982).
    • (1982) Cell , vol.30 , pp. 215-224
    • Benya, P.D.1    Shaffer, J.D.2
  • 17
    • 0025336262 scopus 로고
    • Resting chondrocytes in culture survive without growth factors, but are sensitive to toxic oxygen metabolites
    • Tschan, T. et al. Resting chondrocytes in culture survive without growth factors, but are sensitive to toxic oxygen metabolites. J. Cell Biol. 111, 257-260 (1990).
    • (1990) J. Cell Biol , vol.111 , pp. 257-260
    • Tschan, T.1
  • 18
    • 0029160803 scopus 로고
    • Analysis of collagen synthesis and assembly in culture by immortalized mouse chondrocytes in the presence or absence of alpha 1(IX) collagen chains
    • Mallein-Gerin, F. et al. Analysis of collagen synthesis and assembly in culture by immortalized mouse chondrocytes in the presence or absence of alpha 1(IX) collagen chains. Exp. Cell. Res. 219 257-265 (1995).
    • (1995) Exp. Cell. Res , vol.219 , pp. 257-265
    • Mallein-Gerin, F.1
  • 19
    • 52949100643 scopus 로고    scopus 로고
    • Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression
    • Bougault, C., Paumier, A., Aubert-Foucher, E. & Mallein-Gerin, F. Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression. BMC Biotechnol. 8, 71 (2008).
    • (2008) BMC Biotechnol , vol.8 , pp. 71
    • Bougault, C.1    Paumier, A.2    Aubert-Foucher, E.3    Mallein-Gerin, F.4
  • 20
    • 41149124110 scopus 로고    scopus 로고
    • Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation
    • Barlic, A. et al. Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation. J. Orthop. Res. 9, 9 (2008).
    • (2008) J. Orthop. Res , vol.9 , pp. 9
    • Barlic, A.1
  • 21
    • 25444440612 scopus 로고    scopus 로고
    • Temporal expression patterns and corresponding protein inductions of early responsive genes in rabbit bone marrow-derived mesenchymal stem cells under cyclic compressive loading
    • Huang, C.Y., Reuben, P.M. & Cheung, H.S. Temporal expression patterns and corresponding protein inductions of early responsive genes in rabbit bone marrow-derived mesenchymal stem cells under cyclic compressive loading. Stem Cells 23, 1113-1121 (2005).
    • (2005) Stem Cells , vol.23 , pp. 1113-1121
    • Huang, C.Y.1    Reuben, P.M.2    Cheung, H.S.3
  • 22
    • 33845412455 scopus 로고    scopus 로고
    • Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading
    • Mauck, R.L., Byers, B.A., Yuan, X. & Tuan, R.S. Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading. Biomech. Model. Mechanobiol. 6, 113-125 (2007).
    • (2007) Biomech. Model. Mechanobiol , vol.6 , pp. 113-125
    • Mauck, R.L.1    Byers, B.A.2    Yuan, X.3    Tuan, R.S.4
  • 23
    • 33744724912 scopus 로고    scopus 로고
    • The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage
    • Guilak, F. et al. The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage. Ann. NY Acad. Sci. 1068, 498-512 (2006).
    • (2006) Ann. NY Acad. Sci , vol.1068 , pp. 498-512
    • Guilak, F.1
  • 24
    • 49149126749 scopus 로고    scopus 로고
    • Tensile properties of engineered cartilage formed from chondrocyte- and MSC-laden hydrogels
    • Huang, A.H., Yeger-Mckeever, M., Stein, A. & Mauck, R.L. Tensile properties of engineered cartilage formed from chondrocyte- and MSC-laden hydrogels. Osteoarthr. Cartil. 16, 1074-1082 (2008).
    • (2008) Osteoarthr. Cartil , vol.16 , pp. 1074-1082
    • Huang, A.H.1    Yeger-Mckeever, M.2    Stein, A.3    Mauck, R.L.4
  • 25
    • 56349171203 scopus 로고    scopus 로고
    • Influence of cartilaginous matrix accumulation on viscoelastic response of chondrocyte/agarose constructs under dynamic compressive and shear loading
    • Miyata, S., Tateishi, T. & Ushida, T. Influence of cartilaginous matrix accumulation on viscoelastic response of chondrocyte/agarose constructs under dynamic compressive and shear loading. J. Biomech. Eng. 130, 051016 (2008).
    • (2008) J. Biomech. Eng , vol.130 , pp. 051016
    • Miyata, S.1    Tateishi, T.2    Ushida, T.3
  • 26
    • 0031731809 scopus 로고    scopus 로고
    • The influence of elaborated pericellular matrix on the deformation of isolated articular chondrocytes cultured in agarose
    • Knight, M.M., Lee, D.A. & Bader, D.L. The influence of elaborated pericellular matrix on the deformation of isolated articular chondrocytes cultured in agarose. Biochim. Biophys. Acta 1405 67-77 (1998).
    • (1998) Biochim. Biophys. Acta , vol.1405 , pp. 67-77
    • Knight, M.M.1    Lee, D.A.2    Bader, D.L.3
  • 27
    • 52449090856 scopus 로고    scopus 로고
    • Mechanical stress and prostaglandin E(2) synthesis in cartilage
    • Gosset, M. et al. Mechanical stress and prostaglandin E(2) synthesis in cartilage. Biorheology 45, 301-320 (2008).
    • (2008) Biorheology , vol.45 , pp. 301-320
    • Gosset, M.1
  • 28
    • 0031105663 scopus 로고    scopus 로고
    • Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose
    • Lee, D.A. & Bader, D.L. Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose. J. Orthop. Res. 15, 181-188 (1997).
    • (1997) J. Orthop. Res , vol.15 , pp. 181-188
    • Lee, D.A.1    Bader, D.L.2
  • 29
    • 0034333746 scopus 로고    scopus 로고
    • A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants
    • Frank, E.H. et al. A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants. J. Biomech. 33, 1523-1527 (2000).
    • (2000) J. Biomech , vol.33 , pp. 1523-1527
    • Frank, E.H.1
  • 30
    • 0034210117 scopus 로고    scopus 로고
    • Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels
    • Mauck, R.L. et al. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J. Biomech. Eng. 122, 252-260 (2000).
    • (2000) J. Biomech. Eng , vol.122 , pp. 252-260
    • Mauck, R.L.1
  • 31
    • 0034927281 scopus 로고    scopus 로고
    • Influence of thermal history on the structural and mechanical properties of agarose gels
    • Aymard, P. et al. Influence of thermal history on the structural and mechanical properties of agarose gels. Biopolymers 59, 131-144 (2001).
    • (2001) Biopolymers , vol.59 , pp. 131-144
    • Aymard, P.1
  • 32
    • 10944266990 scopus 로고    scopus 로고
    • Dynamic mechanical properties of agarose gels modeled by a fractional derivative model
    • Chen, Q., Suki, B. & An, K.N. Dynamic mechanical properties of agarose gels modeled by a fractional derivative model. J. Biomech. Eng. 126, 666-671 (2004).
    • (2004) J. Biomech. Eng , vol.126 , pp. 666-671
    • Chen, Q.1    Suki, B.2    An, K.N.3
  • 33
    • 0033745159 scopus 로고    scopus 로고
    • Dynamic pressure transmission through agarose gels
    • Saris, D.B. et al. Dynamic pressure transmission through agarose gels. Tissue Eng. 6, 531-537 (2000).
    • (2000) Tissue Eng , vol.6 , pp. 531-537
    • Saris, D.B.1
  • 34
    • 34248663955 scopus 로고    scopus 로고
    • The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on
    • Chomczynski, P. & Sacchi, N. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat. Protoc. 1, 581-585 (2006).
    • (2006) Nat. Protoc , vol.1 , pp. 581-585
    • Chomczynski, P.1    Sacchi, N.2
  • 35
    • 33645277626 scopus 로고    scopus 로고
    • Tips for extracting total RNA from chondrocytes cultured in agarose gel using a silica-based membrane kit
    • Mio, K., Kirkham, J. & Bonass, W.A. Tips for extracting total RNA from chondrocytes cultured in agarose gel using a silica-based membrane kit. Anal. Biochem. 351, 314-316 (2006).
    • (2006) Anal. Biochem , vol.351 , pp. 314-316
    • Mio, K.1    Kirkham, J.2    Bonass, W.A.3
  • 36
    • 0031742193 scopus 로고    scopus 로고
    • Terminal differentiation of chondrocytes is arrested at distinct stages identified by their expression repertoire of marker genes
    • Szuts, V. et al. Terminal differentiation of chondrocytes is arrested at distinct stages identified by their expression repertoire of marker genes. Matrix Biol. 17, 435-448 (1998).
    • (1998) Matrix Biol , vol.17 , pp. 435-448
    • Szuts, V.1
  • 37
    • 0036145817 scopus 로고    scopus 로고
    • A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage
    • Hoemann, C.D., Sun, J., Chrzanowski, V. & Buschmann, M.D. A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage. Anal. Biochem. 300, 1-10 (2002).
    • (2002) Anal. Biochem , vol.300 , pp. 1-10
    • Hoemann, C.D.1    Sun, J.2    Chrzanowski, V.3    Buschmann, M.D.4
  • 38
    • 39149110297 scopus 로고    scopus 로고
    • Detecting protein-protein interactions by Far western blotting
    • Wu, Y., Li, Q. & Chen, X.Z. Detecting protein-protein interactions by Far western blotting. Nat. Protoc. 2, 3278-3284 (2007).
    • (2007) Nat. Protoc , vol.2 , pp. 3278-3284
    • Wu, Y.1    Li, Q.2    Chen, X.Z.3
  • 39
    • 0034282674 scopus 로고    scopus 로고
    • Regulation of human COL2A1 gene expression in chondrocytes. Identification of C-Krox-responsive elements and modulation by phenotype alteration
    • Ghayor, C. et al. Regulation of human COL2A1 gene expression in chondrocytes. Identification of C-Krox-responsive elements and modulation by phenotype alteration. J. Biol. Chem. 275, 27421-27438 (2000).
    • (2000) J. Biol. Chem , vol.275 , pp. 27421-27438
    • Ghayor, C.1
  • 40
    • 0028071586 scopus 로고
    • Characterization of primary cultures of chondrocytes from type II collagen/beta-galactosidase transgenic mice
    • Lefebvre, V. et al. Characterization of primary cultures of chondrocytes from type II collagen/beta-galactosidase transgenic mice. Matrix Biol. 14, 329-335 (1994).
    • (1994) Matrix Biol , vol.14 , pp. 329-335
    • Lefebvre, V.1
  • 41
    • 0037072760 scopus 로고    scopus 로고
    • Functions of transforming growth factor-beta family type I receptors and Smad proteins in the hypertrophic maturation and osteoblastic differentiation of chondrocytes
    • Valcourt, U. et al. Functions of transforming growth factor-beta family type I receptors and Smad proteins in the hypertrophic maturation and osteoblastic differentiation of chondrocytes. J. Biol. Chem. 277, 33545-33558 (2002).
    • (2002) J. Biol. Chem , vol.277 , pp. 33545-33558
    • Valcourt, U.1
  • 42
    • 50349096522 scopus 로고    scopus 로고
    • Primary culture and phenotyping of murine chondrocytes
    • Gosset, M., Berenbaum, F., Thirion, S. & Jacques, C. Primary culture and phenotyping of murine chondrocytes. Nat. Protoc. 3, 1253-1260 (2008).
    • (2008) Nat. Protoc , vol.3 , pp. 1253-1260
    • Gosset, M.1    Berenbaum, F.2    Thirion, S.3    Jacques, C.4
  • 43
    • 14044269575 scopus 로고    scopus 로고
    • Immature murine articular chondrocytes in primary culture: A new tool for investigating cartilage
    • Salvat, C. et al. Immature murine articular chondrocytes in primary culture: A new tool for investigating cartilage. Osteoarthr. Cartil. 13, 243-249 (2005).
    • (2005) Osteoarthr. Cartil , vol.13 , pp. 243-249
    • Salvat, C.1
  • 44
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl, M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic. Acids. Res. 29, e45 (2001).
    • (2001) Nucleic. Acids. Res , vol.29
    • Pfaffl, M.W.1
  • 45
    • 66749163888 scopus 로고    scopus 로고
    • Sambrook, J., Fritsch, E.F. & Maniatis, T. Detection and analysis of proteins expressed from cloned genes. In Molecular Cloning: A Laboratory Manual 2nd edn 18.19-18.74 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
    • Sambrook, J., Fritsch, E.F. & Maniatis, T. Detection and analysis of proteins expressed from cloned genes. In Molecular Cloning: A Laboratory Manual 2nd edn 18.19-18.74 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
  • 46
    • 23644459677 scopus 로고    scopus 로고
    • Mechanical injury of cartilage explants causes specific time-dependent changes in chondrocyte gene expression
    • Lee, J.H., Fitzgerald, J.B., Dimicco, M.A. & Grodzinsky, A.J. Mechanical injury of cartilage explants causes specific time-dependent changes in chondrocyte gene expression. Arthritis Rheum. 52, 2386-2395 (2005).
    • (2005) Arthritis Rheum , vol.52 , pp. 2386-2395
    • Lee, J.H.1    Fitzgerald, J.B.2    Dimicco, M.A.3    Grodzinsky, A.J.4
  • 47
    • 0037334522 scopus 로고    scopus 로고
    • Microenvironment regulation of extracellular signal-regulated kinase activity in chondrocytes: Effects of culture configuration, interleukin-1, and compressive stress
    • Li, K.W., Wang, A.S. & Sah, R.L. Microenvironment regulation of extracellular signal-regulated kinase activity in chondrocytes: Effects of culture configuration, interleukin-1, and compressive stress. Arthritis Rheum. 48, 689-699 (2003).
    • (2003) Arthritis Rheum , vol.48 , pp. 689-699
    • Li, K.W.1    Wang, A.S.2    Sah, R.L.3
  • 48
    • 0345803943 scopus 로고    scopus 로고
    • Mechanical regulation of mitogen-activated protein kinase signaling in articular cartilage
    • Fanning, P.J. et al. Mechanical regulation of mitogen-activated protein kinase signaling in articular cartilage. J. Biol. Chem. 278, 50940-50948 (2003).
    • (2003) J. Biol. Chem , vol.278 , pp. 50940-50948
    • Fanning, P.J.1
  • 49
    • 33847643776 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RTPCR
    • Nolan, T., Hands, R.E. & Bustin, S.A. Quantification of mRNA using real-time RTPCR. Nat. Protoc. 1, 1559-1582 (2006).
    • (2006) Nat. Protoc , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 50
    • 0027952445 scopus 로고
    • Monoclonal antibody to the aminotelopeptide of type II collagen: Loss of the epitope after stromelysin digestion
    • Mayne, R. et al. Monoclonal antibody to the aminotelopeptide of type II collagen: Loss of the epitope after stromelysin digestion. Connect. Tissue Res. 31, 11-21 (1994).
    • (1994) Connect. Tissue Res , vol.31 , pp. 11-21
    • Mayne, R.1


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