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1
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34147118089
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How electron cryotomography is opening a new window onto prokaryotic ultrastructure
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Two years ago this review discussed ECT as an emerging technology that had just begun to reveal prokaryotic ultrastructure. Covers all relevant papers before 2007.
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Jensen G.J., and Briegel A. How electron cryotomography is opening a new window onto prokaryotic ultrastructure. Curr Opin Struct Biol 17 (2007) 260-267. Two years ago this review discussed ECT as an emerging technology that had just begun to reveal prokaryotic ultrastructure. Covers all relevant papers before 2007.
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(2007)
Curr Opin Struct Biol
, vol.17
, pp. 260-267
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Jensen, G.J.1
Briegel, A.2
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2
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14844330221
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Structural studies by electron tomography: from cells to molecules
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Lucić V., Förster F., and Baumeister W. Structural studies by electron tomography: from cells to molecules. Annu Rev Biochem 74 (2005) 833-865
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(2005)
Annu Rev Biochem
, vol.74
, pp. 833-865
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Lucić, V.1
Förster, F.2
Baumeister, W.3
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3
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20844438496
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Cryo-electron microscopy of vitreous sections
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Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.M., Norlén L.P., Richter K., Blanc N.S., Studer D., and Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J 23 (2004) 3583-3588
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(2004)
EMBO J
, vol.23
, pp. 3583-3588
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Al-Amoudi, A.1
Chang, J.J.2
Leforestier, A.3
McDowall, A.4
Salamin, L.M.5
Norlén, L.P.6
Richter, K.7
Blanc, N.S.8
Studer, D.9
Dubochet, J.10
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4
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30844471175
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Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK
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Komeili A., Li Z., Newman D.K., and Jensen G.J. Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. Science 311 (2006) 242-245
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(2006)
Science
, vol.311
, pp. 242-245
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Komeili, A.1
Li, Z.2
Newman, D.K.3
Jensen, G.J.4
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5
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36248938686
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The structure of FtsZ filaments in vivo suggests a force-generating role in cell division
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Arc-like filaments were seen for the first time at the division plane of C. crescentus cells and identified as the cell division protein FtsZ. Images of the filaments in curved and straight conformations lead to a model wherein FtsZ itself generates force.
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Li Z., Trimble M.J., Brun Y.V., and Jensen G.J. The structure of FtsZ filaments in vivo suggests a force-generating role in cell division. EMBO J 26 (2007) 4694-4708. Arc-like filaments were seen for the first time at the division plane of C. crescentus cells and identified as the cell division protein FtsZ. Images of the filaments in curved and straight conformations lead to a model wherein FtsZ itself generates force.
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(2007)
EMBO J
, vol.26
, pp. 4694-4708
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Li, Z.1
Trimble, M.J.2
Brun, Y.V.3
Jensen, G.J.4
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6
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35148886810
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Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography
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Reports the development of a technique to correlate light and electron microscopy images.
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Lucić V., Kossel A.H., Yang T., Bonhoeffer T., Baumeister W., and Sartori A. Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography. Journal of Structural Biology 160 (2007) 146-156. Reports the development of a technique to correlate light and electron microscopy images.
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(2007)
Journal of Structural Biology
, vol.160
, pp. 146-156
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Lucić, V.1
Kossel, A.H.2
Yang, T.3
Bonhoeffer, T.4
Baumeister, W.5
Sartori, A.6
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9
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45149093047
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Location and architecture of the Caulobacter crescentus chemoreceptor array
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•].
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•].
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(2008)
Mol Microbiol
, vol.69
, pp. 30-41
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Briegel, A.1
Ding, H.2
Li, Z.3
Werner, J.4
Gitai, Z.5
Dias, D.6
Jensen, R.7
Jensen, G.J.8
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10
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58249090246
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The native 3D organization of bacterial polysomes
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ECT and a template-matching were used to reveal the arrangement of 70S ribosomes in translating polysomes from E. coli-derived translation lysates. The arrangement was suggested to increase the efficiency of nascent polypeptide folding.
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Brandt F., Etchells S.A., Ortiz J.O., Elcock A.H., Hartl F.U., and Baumeister W. The native 3D organization of bacterial polysomes. Cell 136 (2009) 261-271. ECT and a template-matching were used to reveal the arrangement of 70S ribosomes in translating polysomes from E. coli-derived translation lysates. The arrangement was suggested to increase the efficiency of nascent polypeptide folding.
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(2009)
Cell
, vol.136
, pp. 261-271
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Brandt, F.1
Etchells, S.A.2
Ortiz, J.O.3
Elcock, A.H.4
Hartl, F.U.5
Baumeister, W.6
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11
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58849121358
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Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation
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Vitreous sectioning and ECT were used to show that the ParM filaments responsible for segregating R1-derived plasmids are arranged in small bundles in the periphery of the nucleoid.
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Salje J., Zuber B., and Lowe J. Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation. Science 323 (2009) 509-512. Vitreous sectioning and ECT were used to show that the ParM filaments responsible for segregating R1-derived plasmids are arranged in small bundles in the periphery of the nucleoid.
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(2009)
Science
, vol.323
, pp. 509-512
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Salje, J.1
Zuber, B.2
Lowe, J.3
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14
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0031926887
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Electron tomography of ice-embedded prokaryotic cells
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Grimm R., Singh H., Rachel R., Typke D., Zillig W., and Baumeister W. Electron tomography of ice-embedded prokaryotic cells. Biophys J 74 (1998) 1031-1042
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(1998)
Biophys J
, vol.74
, pp. 1031-1042
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Grimm, R.1
Singh, H.2
Rachel, R.3
Typke, D.4
Zillig, W.5
Baumeister, W.6
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15
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39149141843
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The bacterial cytoskeleton
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Pogliano J. The bacterial cytoskeleton. Curr Opin Cell Biol 20 (2008) 19-27
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(2008)
Curr Opin Cell Biol
, vol.20
, pp. 19-27
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Pogliano, J.1
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16
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0026059127
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FtsZ ring structure associated with division in Escherichia coli
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Bi E.F., and Lutkenhaus J. FtsZ ring structure associated with division in Escherichia coli. Nature 354 (1991) 161-164
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(1991)
Nature
, vol.354
, pp. 161-164
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Bi, E.F.1
Lutkenhaus, J.2
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17
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0029918758
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Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis
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Levin P.A., and Losick R. Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis. Genes Dev 10 (1996) 478-488
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(1996)
Genes Dev
, vol.10
, pp. 478-488
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Levin, P.A.1
Losick, R.2
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18
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0030829601
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FtsZ, a tubulin homologue in prokaryote cell division
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Erickson H.P. FtsZ, a tubulin homologue in prokaryote cell division. Trends Cell Biol 7 (1997) 362-367
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(1997)
Trends Cell Biol
, vol.7
, pp. 362-367
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Erickson, H.P.1
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19
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41549137007
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Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography
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ECT revealed the 3D architecture and cell shape deformation of predator Bdellovibrio bacteriovorus cells.
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Borgnia M.J., Subramaniam S., and Milne J.L. Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography. J Bacteriol 190 (2008) 2588-2596. ECT revealed the 3D architecture and cell shape deformation of predator Bdellovibrio bacteriovorus cells.
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(2008)
J Bacteriol
, vol.190
, pp. 2588-2596
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Borgnia, M.J.1
Subramaniam, S.2
Milne, J.L.3
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20
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45549101208
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Native cellular architecture of Treponema denticola revealed by cryo-electron tomography
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ECT revealed the native cellular architecture of the pathogenic spirochete Treponema denticola.
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Izard J., Hsieh C.E., Limberger R.J., Mannella C.A., and Marko M. Native cellular architecture of Treponema denticola revealed by cryo-electron tomography. J Struct Biol 163 (2008) 10-17. ECT revealed the native cellular architecture of the pathogenic spirochete Treponema denticola.
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(2008)
J Struct Biol
, vol.163
, pp. 10-17
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Izard, J.1
Hsieh, C.E.2
Limberger, R.J.3
Mannella, C.A.4
Marko, M.5
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21
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53849119130
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Chemoreceptors in Caulobacter crescentus: trimers of receptor dimers in a partially ordered hexagonally packed array
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•], but emphasized that the arrays were not perfectly hexagonal.
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•], but emphasized that the arrays were not perfectly hexagonal.
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(2008)
J Bacteriol
, vol.190
, pp. 6805-6810
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Khursigara, C.1
Wu, X.2
Subramaniam, S.3
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22
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57749083521
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Molecular organization of Gram-negative peptidoglycan
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ECT of Gram-negative sacculi revealed that the glycan strands lie in a single layer parallel to the cell membranes, roughly perpendicular to the long axis of the cell.
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Gan L., Chen S., and Jensen G.J. Molecular organization of Gram-negative peptidoglycan. Proc Natl Acad Sci U S A 105 (2008) 18953-18957. ECT of Gram-negative sacculi revealed that the glycan strands lie in a single layer parallel to the cell membranes, roughly perpendicular to the long axis of the cell.
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(2008)
Proc Natl Acad Sci U S A
, vol.105
, pp. 18953-18957
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Gan, L.1
Chen, S.2
Jensen, G.J.3
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23
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39849105856
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Novel ultrastructures of Treponema primitia and their implications for motility
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ECT of a recently cultured termide gut spirochete Treponema primitia revealed rich subcellular structures. These results, together with the in situ structure of the flagellar motor from the same species reported in [24], support the 'rolling cylinder' model of spirochete motility
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Murphy G.E., Matson E.G., Leadbetter J.R., Berg H.C., and Jensen G.J. Novel ultrastructures of Treponema primitia and their implications for motility. Mol Microbiol 67 (2008) 1184-1195. ECT of a recently cultured termide gut spirochete Treponema primitia revealed rich subcellular structures. These results, together with the in situ structure of the flagellar motor from the same species reported in [24], support the 'rolling cylinder' model of spirochete motility
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(2008)
Mol Microbiol
, vol.67
, pp. 1184-1195
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Murphy, G.E.1
Matson, E.G.2
Leadbetter, J.R.3
Berg, H.C.4
Jensen, G.J.5
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24
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33748295677
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In situ structure of the complete Treponema primitia flagellar motor
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Murphy G.E., Leadbetter J.R., and Jensen G.J. In situ structure of the complete Treponema primitia flagellar motor. Nature 442 (2006) 1062-1064
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(2006)
Nature
, vol.442
, pp. 1062-1064
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Murphy, G.E.1
Leadbetter, J.R.2
Jensen, G.J.3
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25
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0017123749
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How spirochetes may swim
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Berg H.C. How spirochetes may swim. J Theor Biol 56 (1976) 269-273
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(1976)
J Theor Biol
, vol.56
, pp. 269-273
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Berg, H.C.1
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26
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0035138176
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Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects
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Izard J., Samsonoff W.A., and Limberger R.J. Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects. J Bacteriol 183 (2001) 1078-1084
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(2001)
J Bacteriol
, vol.183
, pp. 1078-1084
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Izard, J.1
Samsonoff, W.A.2
Limberger, R.J.3
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27
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58649114723
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The flat ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology
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ECT of the Lyme disease spirochete Borrelia burgdorferi revealed that the periplasmic flagella form a right-handed ribbon rather than a bundle, as was previously suggested by traditional EM.
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Charon N.W., Goldstein S.F., Marko M., Hsieh C., Gebhardt L.L., Motaleb M.A., Wolgemuth C.W., Limberger R.J., and Rowe N. The flat ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology. J Bacteriol 191 (2009) 600-607. ECT of the Lyme disease spirochete Borrelia burgdorferi revealed that the periplasmic flagella form a right-handed ribbon rather than a bundle, as was previously suggested by traditional EM.
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(2009)
J Bacteriol
, vol.191
, pp. 600-607
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Charon, N.W.1
Goldstein, S.F.2
Marko, M.3
Hsieh, C.4
Gebhardt, L.L.5
Motaleb, M.A.6
Wolgemuth, C.W.7
Limberger, R.J.8
Rowe, N.9
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28
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0034718538
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Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions
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Motaleb M.A., Corum L., Bono J.L., Elias A.F., Rosa P., Samuels D.S., and Charon N.W. Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions. Proc Natl Acad Sci U S A 97 (2000) 10899-10904
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(2000)
Proc Natl Acad Sci U S A
, vol.97
, pp. 10899-10904
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Motaleb, M.A.1
Corum, L.2
Bono, J.L.3
Elias, A.F.4
Rosa, P.5
Samuels, D.S.6
Charon, N.W.7
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29
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35048829921
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Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography
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ECT and genetic analysis revealed tufts of ∼5-nm-wide cell surface filaments that appeared to be anchored to the inner surface of the outer membrane in wild-type cells. These filaments are absent in nonmotile gldF mutant cells, suggesting that these filaments are involved in gliding motility.
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Liu J., McBride M.J., and Subramaniam S. Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. J Bacteriol 189 (2007) 7503-7506. ECT and genetic analysis revealed tufts of ∼5-nm-wide cell surface filaments that appeared to be anchored to the inner surface of the outer membrane in wild-type cells. These filaments are absent in nonmotile gldF mutant cells, suggesting that these filaments are involved in gliding motility.
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(2007)
J Bacteriol
, vol.189
, pp. 7503-7506
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Liu, J.1
McBride, M.J.2
Subramaniam, S.3
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30
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33745057437
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Inch by inch, row by row
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Weis R.M. Inch by inch, row by row. Nat Struct Mol Biol 13 (2006) 382-384
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(2006)
Nat Struct Mol Biol
, vol.13
, pp. 382-384
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Weis, R.M.1
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31
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34247261989
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Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy
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This paper used ECT to directly visualize chemotaxis receptor arrays in wild-type E. coli and mutant strains over-expressing the serine chemoreceptor Tsr. Combined with biochemical data, the authors demonstrated that the signaling molecules CheA and CheW are required for functional chemotaxis arrays.
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Zhang P., Khursigara C.M., Hartnell L.M., and Subramaniam S. Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy. Proc Natl Acad Sci U S A 104 (2007) 3777-3781. This paper used ECT to directly visualize chemotaxis receptor arrays in wild-type E. coli and mutant strains over-expressing the serine chemoreceptor Tsr. Combined with biochemical data, the authors demonstrated that the signaling molecules CheA and CheW are required for functional chemotaxis arrays.
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(2007)
Proc Natl Acad Sci U S A
, vol.104
, pp. 3777-3781
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Zhang, P.1
Khursigara, C.M.2
Hartnell, L.M.3
Subramaniam, S.4
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32
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55949115430
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Role of HAMP domains in chemotaxis signaling by bacterial chemoreceptors
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ECT and 3D image averaging revealed two distinct conformations of the HAMP domain of Tsr chemoreceptors, suggesting a possible molecular mechanism in chemotaxis signaling.
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Khursigara C.M., Wu X., Zhang P., Lefman J., and Subramaniam S. Role of HAMP domains in chemotaxis signaling by bacterial chemoreceptors. Proc Natl Acad Sci U S A 105 (2008) 16555-16560. ECT and 3D image averaging revealed two distinct conformations of the HAMP domain of Tsr chemoreceptors, suggesting a possible molecular mechanism in chemotaxis signaling.
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(2008)
Proc Natl Acad Sci U S A
, vol.105
, pp. 16555-16560
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Khursigara, C.M.1
Wu, X.2
Zhang, P.3
Lefman, J.4
Subramaniam, S.5
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33
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49649111573
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Protein-based organelles in bacteria: carboxysomes and related microcompartments
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Yeates T.O., Kerfeld C.A., Heinhorst S., Cannon G.C., and Shively J.M. Protein-based organelles in bacteria: carboxysomes and related microcompartments. Nat Rev Microbiol 6 (2008) 681-691
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(2008)
Nat Rev Microbiol
, vol.6
, pp. 681-691
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Yeates, T.O.1
Kerfeld, C.A.2
Heinhorst, S.3
Cannon, G.C.4
Shively, J.M.5
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36
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34250338914
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Cryo-electron tomography reveals the comparative three-dimensional architecture of Prochlorococcus, a globally important marine cyanobacterium
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This paper applied whole-cell ECT, ECT of vitreous sections, and comparative genomic analysis to characterize two closely related strains of cyanobacterium Prochlorococcus.
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Ting C.S., Hsieh C., Sundararaman S., Mannella C., and Marko M. Cryo-electron tomography reveals the comparative three-dimensional architecture of Prochlorococcus, a globally important marine cyanobacterium. J Bacteriol 189 (2007) 4485-4493. This paper applied whole-cell ECT, ECT of vitreous sections, and comparative genomic analysis to characterize two closely related strains of cyanobacterium Prochlorococcus.
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(2007)
J Bacteriol
, vol.189
, pp. 4485-4493
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Ting, C.S.1
Hsieh, C.2
Sundararaman, S.3
Mannella, C.4
Marko, M.5
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37
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40649085591
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Structural analysis of photosynthetic membranes by cryo-electron tomography of intact Rhodopseudomonas viridis cells
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ECT of the purple photosynthetic bacterium Rhodopseudomonas viridis revealed closely packed, flattened photosynthetic membranes connected to the inner membrane by tunnel-like invaginations.
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Konorty M., Kahana N., Linaroudis A., Minsky A., and Medalia O. Structural analysis of photosynthetic membranes by cryo-electron tomography of intact Rhodopseudomonas viridis cells. J Struct Biol 161 (2008) 393-400. ECT of the purple photosynthetic bacterium Rhodopseudomonas viridis revealed closely packed, flattened photosynthetic membranes connected to the inner membrane by tunnel-like invaginations.
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(2008)
J Struct Biol
, vol.161
, pp. 393-400
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Konorty, M.1
Kahana, N.2
Linaroudis, A.3
Minsky, A.4
Medalia, O.5
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38
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62649088069
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Chromatin organization and radio-resistance in the bacterium Gemmata obscuriglobus
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ECT of frozen-hydrated sections of Gemmata obscuriglobus (a member of the phylum Planctomycetes) revealed a network of double-membrane compartments that probably enclose packed chromatin, an organization speculated to increase the radiation tolerance of this species.
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Lieber A., Leis A., Kushmaro A., Minsky A., and Medalia O. Chromatin organization and radio-resistance in the bacterium Gemmata obscuriglobus. J Bacteriol 191 (2009) 1439-1445. ECT of frozen-hydrated sections of Gemmata obscuriglobus (a member of the phylum Planctomycetes) revealed a network of double-membrane compartments that probably enclose packed chromatin, an organization speculated to increase the radiation tolerance of this species.
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(2009)
J Bacteriol
, vol.191
, pp. 1439-1445
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Lieber, A.1
Leis, A.2
Kushmaro, A.3
Minsky, A.4
Medalia, O.5
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39
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49749094746
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Ignicoccus hospitalis and Nanoarchaeum equitans: ultrastructure, cell-cell interaction, and 3D reconstruction from serial sections of freeze-substituted cells and by electron cryotomography
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This paper describes the 3D ultrastructure of Ignicoccus hospitalis and Nanoarchaeum equitans and their cell-cell interaction.
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Junglas B., Briegel A., Burghardt T., Walther P., Wirth R., Huber H., and Rachel R. Ignicoccus hospitalis and Nanoarchaeum equitans: ultrastructure, cell-cell interaction, and 3D reconstruction from serial sections of freeze-substituted cells and by electron cryotomography. Arch Microbiol 190 (2008) 395-408. This paper describes the 3D ultrastructure of Ignicoccus hospitalis and Nanoarchaeum equitans and their cell-cell interaction.
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(2008)
Arch Microbiol
, vol.190
, pp. 395-408
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Junglas, B.1
Briegel, A.2
Burghardt, T.3
Walther, P.4
Wirth, R.5
Huber, H.6
Rachel, R.7
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40
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58549108582
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Three-dimensional analysis of the structure and ecology of a novel, ultra-small archaeon
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This paper describes the 3D ultrastructure of an uncultivated, ultra-small archaeon in acid mine drainage (AMD) biofilm samples.
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Comolli L.R., Baker B.J., Downing K.H., Siegerist C.E., and Banfield J.F. Three-dimensional analysis of the structure and ecology of a novel, ultra-small archaeon. ISME J 3 (2009) 159-167. This paper describes the 3D ultrastructure of an uncultivated, ultra-small archaeon in acid mine drainage (AMD) biofilm samples.
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(2009)
ISME J
, vol.3
, pp. 159-167
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Comolli, L.R.1
Baker, B.J.2
Downing, K.H.3
Siegerist, C.E.4
Banfield, J.F.5
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41
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33847214237
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Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain
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Burns D.G., Janssen P.H., Itoh T., Kamekura M., Li Z., Jensen G., Rodríguez-Valera F., Bolhuis H., and Dyall-Smith M.L. Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain. Int J Syst Evol Microbiol 57 (2007) 387-392
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(2007)
Int J Syst Evol Microbiol
, vol.57
, pp. 387-392
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Burns, D.G.1
Janssen, P.H.2
Itoh, T.3
Kamekura, M.4
Li, Z.5
Jensen, G.6
Rodríguez-Valera, F.7
Bolhuis, H.8
Dyall-Smith, M.L.9
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42
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37449006022
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Tetrasphaera remsis sp. nov., isolated from the regenerative enclosed life support module simulator (REMS) air system.
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Osman S., Moissl C., Hosoya N., Briegel A., Mayilraj S., Satomi M., and Venkateswaran K. Tetrasphaera remsis sp. nov., isolated from the regenerative enclosed life support module simulator (REMS) air system. Int J Syst Evol Microbiol 57 (2007) 2749-2753
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(2007)
Int J Syst Evol Microbiol
, vol.57
, pp. 2749-2753
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Osman, S.1
Moissl, C.2
Hosoya, N.3
Briegel, A.4
Mayilraj, S.5
Satomi, M.6
Venkateswaran, K.7
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43
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51549102573
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A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter
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Ebersbach G., Briegel A., Jensen G.J., and Jacobs-Wagner C. A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter. Cell 134 (2008) 956-968
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(2008)
Cell
, vol.134
, pp. 956-968
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Ebersbach, G.1
Briegel, A.2
Jensen, G.J.3
Jacobs-Wagner, C.4
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44
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51549087758
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