Forskolin decreases phosphorylation of histone H2AX in human cells induced by ionizing radiation

Radiation Research

Volumn 171, Issue 4, 2009, Pages 419-424

  Solovjeva, L V ^a   Pleskach, N M ^a   Firsanov, D V ^a   Svetlova, M P ^a   Serikov, V B ^b   Tomilin, N V ^a  

^a INSTITUTE OF CYTOLOGY   (Russian Federation)

^b CHILDREN S HOSPITAL OAKLAND RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FORSKOLIN; HISTONE H2AX; CYCLINE; H2AFX PROTEIN, HUMAN; HISTONE;

ARTICLE; CELL NUCLEUS; CELL SURVIVAL; CHROMOSOME ABERRATION; CONTROLLED STUDY; EMBRYO; FIBROBLAST; FLUORESCENCE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IONIZING RADIATION; IRRADIATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RADIATION INJURY; X IRRADIATION; ANIMAL; CHEMISTRY; CHROMATIN; CRICETULUS; HAMSTER; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; RADIATION EXPOSURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

COLEUS;

ANIMALS; CELL NUCLEUS; CELL SURVIVAL; CHROMATIN; CHROMOSOME ABERRATIONS; CRICETINAE; CRICETULUS; FORSKOLIN; HISTONES; HUMANS; PHOSPHORYLATION; PROLIFERATING CELL NUCLEAR ANTIGEN; PROTEIN STRUCTURE, TERTIARY; RADIATION, IONIZING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 66149141077     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR1587.1     Document Type: Article

References (28)

1. E. P. Rogakou, D. R. Pilch, A. H. Orr, V. S. Ivanova and W. M. Bonner, DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem. 273, 5858-5868 (1998).
2. E. P. Rogakou, C. Boon, C. Redon and W. M. Bonner, Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
3. N. V. Tomilin, L. V. Solovjeva, M. P. Svetlova, N. M. Pleskach, I. A. Zalenskaya, P. M. Yau and E. M. Bradbury, Visualization of focal nuclear sites of DNA repair synthesis induced by bleomycin in human cells. Radiat. Res. 156, 347-354 (2001).
4. T. Ikura, S. Tashiro, A. Kakino, H. Shima, N. Jacob, R. Amunugama, K. Yoder, S. Izumi, I. Kuraoka and K. Kamiya, DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics. Mol. Cell Biol. 27, 7028-7040 (2007).
5. S. Jha, E. Shibata and A. Dutta, Human Rvb1/Tip49 is required for the histone acetyltransferase activity of Tip60/NuA4 and for the downregulation of phosphorylation on H2AX after DNA damage. Mol. Cell. Biol. 28, 2690-2700 (2008).
6. I. B. Nazarov, A. N. Smirnova, R. I. Krutilina, M. P. Svetlova, L. V. Solovjeva, A. A. Nikiforov, S. L. Oei, I. A. Zalenskaya, P. M. Yau and N. V. Tomilin, Dephosphorylation of histone gamma-H2AX during repair of DNA double-strand breaks in mammalian cells and its inhibition by calyculin A. Radiat. Res. 160, 309-317 (2003).
7. M. Svetlova, L. Solovjeva, K. Nishi, I. Nazarov, J. Siino and N. Tomilin, Elimination of radiation-induced gamma-H2AX foci in mammalian nucleus can occur by histone exchange. Biochem. Biophys. Res. Commun. 358, 650-654 (2007).
8. D. Chowdhury, M. C. Keogh, H. Ishii, C. L. Peterson, S. Buratowski and J. Lieberman, Gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Mol. Cell 20, 801-809 (2005).
9. J. H. Ahn, T. McAvoy, S. V. Rakhilin, A. Nishi, P. Greengard and A. C. Nairn, Protein kinase A activates protein phosphatase 2A by phosphorylation of the B56delta subunit. Proc. Natl. Acad. Sci. USA 104, 2979-2984 (2007).
10. A. Laurenza, E. M. Sutkowski and K. B. Seamon, Forskolin: A specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action? Trends Prot. Sci. 101, 442-446 (1989).
11. K. B. Seamon, W. Padgett and W. Daly, Forskolin: Unique diterpene activator of adenylate cyclases in membranes and in intact cells. Proc. Natl. Acad. Sci. USA 78, 3363-3367 (1981).
12. M. S. Feschenko, E. Stevenson, A. C. Nairn and K. J. Sweadner, A novel cAMP-stimulated pathway in protein phosphatase 2A activation. J. Pharmacol. Exp. Ther. 302, 11-118 (2002).
13. D. Perrpotti and P. Neviani, Protein phosphatase 2A (PP2A), a drugable tumor suppressor in Ph1(+) leukemias. Cancer Metastasis Rev. 27, 159-168 (2008).
14. P. Neviani, R. Santhanam, R. Trotta, M. Notari, B. W. Blaser, S. Liu, H. Mao, J. S. Chang, A. Galietta and D. Perrotti, The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein. Cancer Cell 8, 355-368 (2005).
15. N. P. Shanware, A. T. Trinh, L. M. Williams and R. S. Tibbetts, Coregulated ataxia telangiectasia-mutated and casein kinase sites modulate cAMP-response element-binding protein-coactivator interactions in response to DNA damage. J. Biol. Chem. 282, 6283-6291 (2007).
16. C. J. Bakkenist and M. B. Kastan, DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506 (2003).
17. R. S. Maser, K. J. Monsen, B. E. Nelms and J. H. Petrini, hMre11 and hRad50 nuclear foci are induced during the normal cellular response to DNA double-strand breaks. Mol. Cell. Biol. 17, 6087-6096 (1997).
18. J. H. Lee and T. T. Paull, Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex. Science 304, 93-96 (2004).
19. C. Lukas, F. Melander, M. Stucki, J. Falck, S. Bekker-Jensen, M. Goldberg, Y. Lerenthal, S. P. Jackson, J. Bartek and J. Lukas, Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention. EMBO J. 23, 2674-2683 (2004).
20. S. Bekker-Jensen, C. Lukas, R. Kitagawa, F. Melander, M. B. Kastan, J. Bartek and J. Lukas, Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. J. Cell. Biol. 173, 195-206 (2006).
21. Y. Sun, Y. Xu, K. Roy and B. D. Price, DNA damage-induced acetylation of lysine 3016 of ATM activates ATM kinase activity. Mol. Cell. Biol. 27, 8502-8509 (2007).
22. E. W. Englander and S. H. Wilson, DNA damage response of cloned DNA beta-polymerase promoter is blocked in mutant cell lines deficient in protein kinase A. Nucleic Acids Res. 20, 5527-5531 (1992).
23. C. Vens, I. Hofland and A. C. Begg, Involvement of DNA polymerase beta in repair of ionizing radiation damage as measured by in vitro plasmid assays. Radiat Res. 168, 281-291 (2007).
24. G. Taucher-Scholz and G. Kraft, Influence of radiation quality on the yield of DNA strand breaks in SV40 DNA irradiated in solution. Radiat Res. 151, 595-604 (1999).
25. X. Tang, Z. G. Hui, X. L. Cui, R. Garg, M. B. Kastan and B. Xu, A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol. Cell. Biol. 28, 2559-2566 (2008).
26. T. Kusch, L. Florens, W. H. Macdonald, S. K. Swanson, R. L. Glaser, J. R. Yates, S. M. Abmayr, M. P. Washburn and J. L. Workman, Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions. Science 306, 2084-2087 (2004).
27. K. Heo, H. Kim, S. H. Choi, J. Choi, K. Kim, J. Gu, M. R. Lieber, A. S. Yang and W. An, FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol. Cell 30, 86-97 (2008).
28. J. S. Siino, I. B. Nazarov, M. P. Svetlova, L. V. Solovjeva, R. H. Adamson, I. A. Zalenskaya, P. M. Yau, E. M. Bradbury and N. V. Tomilin, Photobleaching of GFP-labeled H2AX in chromatin: H2AX has low diffusional mobility in the nucleus. Biochem. Biophys. Res. Commun. 297, 1318-1323 (2002).