Steroid and protein ligand binding to cytochrome P450 46A1 as assessed by hydrogen-deuterium exchange and mass spectrometry

Biochemistry

Volumn 48, Issue 19, 2009, Pages 4150-4158

  Liao, Wei Li ^a,b   Dodder, Nathan G ^b   Mast, Natalia ^c   Pikuleva, Irina A ^c   Turko, Illarion V ^a,b,c  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ADRENODOXIN; AMIDE PROTON; CYTOCHROME P450; DEUTERATION; ENZYME ACTIVE SITES; H-D EXCHANGE; HYDROGEN-DEUTERIUM EXCHANGE; KEY ENZYMES; LIGAND INTERACTIONS; PROTEIN LIGANDS; REDOX PARTNERS; STEROID SUBSTRATE; STRUCTURAL INSIGHTS; SUBSTRATE BINDING; SUBSTRATE-FREE;

AMIDES; AMINES; BINDING ENERGY; BINDING SITES; CHOLESTEROL; DEUTERIUM; ENZYMES; HYDROGEN; LIGANDS; MASS SPECTROMETERS; MASS SPECTROMETRY; PEPTIDES; REACTION KINETICS; SPECTRUM ANALYSIS;

SUBSTRATES;

ADRENODOXIN; CHOLESTEROL; CHOLESTEROL DERIVATIVE; CYTOCHROME P450 46A1; CYTOCHROME P450 ISOENZYME; SULFATE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DEUTERIUM HYDROGEN EXCHANGE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SUBSTRATE COMPLEX; HORMONE PROTEIN COMPLEX; LIGAND BINDING; MASS SPECTROMETRY; MOLECULAR INTERACTION; OXIDATION REDUCTION REACTION; PEPTIDE MAPPING; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STEROID BINDING;

ADRENODOXIN; AMINO ACID SEQUENCE; BINDING SITES; CHOLESTEROL ESTERS; CYTOCHROME P-450 ENZYME SYSTEM; DEUTERIUM; HYDROGEN; HYDROXYCHOLESTEROLS; LIGANDS; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PEPTIDE MAPPING; PROTEIN BINDING; PROTEINS; STEROIDS; SUBSTRATE SPECIFICITY;

EID: 66049156166     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900168m     Document Type: Article

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