Withaferin A induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes

BioFactors

Volumn 33, Issue 2, 2008, Pages 137-148

  Park, Hea Jin ^b,d   Rayalam, Srujana ^b   Della Fera, Mary Anne ^b   Ambati, Suresh ^b   Yang, Jeong Yeh ^b   Baile, Clifton A ^a,b,c  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c UNIVERSITY OF GEORGIA   (United States)

^d UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

AP2; Bcl2 family; ERK1 2; PPAR ; Withania somnifera

Indexed keywords

CCAAT ENHANCER BINDING PROTEIN ALPHA; FATTY ACID BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PROTEIN BAX; PROTEIN BCL 2; SINGLE STRANDED DNA; WITHAFERIN A;

ADIPOGENESIS; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL MATURATION; CELL VIABILITY; CONTROLLED STUDY; DOSE RESPONSE; DRUG MECHANISM; DRUG STRUCTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; LIPID STORAGE; NONHUMAN; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; ANGIOGENESIS INHIBITORS; ANIMALS; APOPTOSIS; CCAAT-ENHANCER-BINDING PROTEIN-ALPHA; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ERGOSTEROL; MICE; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; PHOSPHORYLATION; PPAR GAMMA; TIME FACTORS;

WITHANIA SOMNIFERA;

EID: 65949085789     PISSN: 09516433     EISSN: None     Source Type: Journal    
DOI: 10.1002/biof.5520330206     Document Type: Article

References (33)

1. G.C. Chang, S.L. Hsu, J.R. Tsai, W.J. Wu, C.Y. Chen and G.T. Sheu, Extracellular signal-regulated kinase activation and Bcl-2 downregulation mediate apoptosis after gemcitabine treatment partly via a p53-independent pathway, Eur J Pharmacol 502 (2004), 169-183.
2. G.W. Commons, B. Halperin and C.C. Chang, Large-volume liposuction: a review of 631 consecutive cases over 12 years, Plast Reconstr Surg 108 (2001), 1753-1763, discussion 1764-1757.
3. O.S. Frankfurt, Immunoassay for single-stranded DNA in apoptotic cells, Methods Mol Biol 282 (2004), 85-101.
4. S. Ghatan, S. Larner, Y. Kinoshita, M. Hetman, L. Patel, Z. Xia, R.J. Youle and R.S. Morrison, p38 MAP kinase mediates bax translocation in nitric oxide-induced apoptosis in neurons, J Cell Biol 150 (2000), 335-347.
5. F.M. Gregoire, Adipocyte differentiation: from fibroblast to endocrine cell, Exp Biol Med (Maywood) 226 (2001), 997-1002.
6. F.M. Gregoire, C.M. Smas and H.S. Sul, Understanding adipocyte differentiation, Physiol Rev 78 (1998), 783-809.
7. S.S. Grewal, R.D. York and P.J. Stork, Extracellular-signal-regulated kinase signalling in neurons, Curr Opin Neurobiol 9 (1999), 544-553.
8. A. Harmon and J. Harp, Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis, Am J Physiol Cell Physiol 280 (2001), C807-813.
9. N. Hemati, S.E. Ross, R.L. Erickson, G.E. Groblewski and O.A. MacDougald, Signaling pathways through which insulin regulates CCAAT/enhancer binding protein alpha (C/EBPalpha) phosphorylation and gene expression in 3T3-L1 adipocytes. Correlation with GLUT4 gene expression, J Biol Chem 272 (1997), 25913-25919.
10. C. Huang, Y. Zhang, Z. Gong, X. Sheng, Z. Li, W. Zhang and Y. Qin, Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway, Biochem Biophys Res Commun 348 (2006), 571-578.
11. N. Joza, G. Kroemer and J.M. Penninger, Genetic analysis of the mammalian cell death machinery, Trends Genet 18 (2002), 142-149.
12. M. Kaileh, W. Vanden Berghe, A. Heyerick, J. Horion, J. Piette, C. Libert, D. De Keukeleire, T. Essawi and G. Haegeman, Withaferin a strongly elicits IkappaB kinase beta hyperphosphorylation concomitant with potent inhibition of its kinase activity, J Biol Chem 282 (2007), 4253-4264.
13. H.K. Kim, C. Nelson-Dooley, M.A. Della-Fera, J.Y. Yang, W. Zhang, J. Duan, D.L. Hartzell, M.W. Hamrick and C.A. Baile, Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice, J Nutr 136 (2006), 409-414.
14. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970), 680-685.
15. F. Malik, A. Kumar, S. Bhushan, S. Khan, A. Bhatia, K.A. Suri, G.N. Qazi and J. Singh, Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic cell death of human myeloid leukemia HL-60 cells by a dietary compound withaferin A with concomitant protection by N-acetyl cysteine, Apoptosis 12 (2007), 2115-2133.
16. L.C. Mishra, B.B. Singh and S. Dagenais, Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review, Altern Med Rev 5 (2000), 334-346.
17. S. Mohr, T.S. McCormick and E.G. Lapetina, Macrophages resistant to endogenously generated nitric oxide-mediated apoptosis are hypersensitive to exogenously added nitric oxide donors: dichotomous apoptotic response independent of caspase 3 and reversal by the mitogen-activated protein kinase kinase (MEK) inhibitor PD 098059, Proc Natl Acad Sci USA 95 (1998), 5045-5050.
18. H.S. Moon, H.G. Lee, Y.J. Choi, T.G. Kim and C.S. Cho, Proposed mechanisms of (-)-epigallocatechin-3-gallate for anti-obesity, Chem Biol Interact (2007).
19. J.D. Paulauskis and H.S. Sul, Cloning and expression of mouse fatty acid synthase and other specific mRNAs. Developmental and hormonal regulation in 3T3-L1 cells, J Biol Chem 263 (1988), 7049-7054.
20. J.B. Prins and S. O'Rahilly, Regulation of adipose cell number in man, Clin Sci (Lond) 92 (1997), 3-11.
21. J.B. Prins, N.I. Walker, C.M. Winterford and D.P. Cameron, Human adipocyte apoptosis occurs in malignancy, Biochem Biophys Res Commun 205 (1994), 625-630.
22. D.A. Roncari, D.C. Lau and S. Kindler, Exaggerated replication in culture of adipocyte precursors from massively obese persons, Metabolism 30 (1981), 425-427.
23. E.D. Rosen, C.H. Hsu, X. Wang, S. Sakai, M.W. Freeman, F.J. Gonzalez and B.M. Spiegelman, C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway, Genes Dev 16 (2002), 22-26.
24. N. Sen, B. Banerjee, B.B. Das, A. Ganguly, T. Sen, S. Pramanik, S. Mukhopadhyay and H.K. Majumder, Apoptosis is induced in leishmanial cells by a novel protein kinase inhibitor withaferin A and is facilitated by apoptotic topoisomerase I-DNA complex, Cell Death Differ 14 (2007), 358-367.
25. B.M. Spiegelman, M. Frank and H. Green, Molecular cloning of mRNA from 3T3 adipocytes. Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation-dependent proteins during adipocyte development, J Biol Chem 258 (1983), 10083-10089.
26. S. Srinivasan, R.S. Ranga, R. Burikhanov, S.S. Han and D. Chendil, Par-4-dependent apoptosis by the dietary compound withaferin A in prostate cancer cells, Cancer Res 67 (2007), 246-253.
27. A. Suryawan and C.Y. Hu, Effect of serum on differentiation of porcine adipose stromal-vascular cells in primary culture, Comp Biochem Physiol Comp Physiol 105 (1993), 485-492.
28. M. Winters, Ancient medicine, modern use: Withania somnifera and its potential role in integrative oncology, Altern Med Rev 11 (2006), 269-277.
29. H. Yang, G. Shi and Q.P. Dou, The tumor proteasome is a primary target for the natural anticancer compound Withaferin A isolated from Indian winter cherry, Mol Pharmacol 71 (2007), 426-437.
30. J.Y. Yang, M.A. Della-Fera and C.A. Baile, Esculetin induces mitochondria-mediated apoptosis in 3T3-L1 adipocytes, Apoptosis 11 (2006), 1371-1378.
31. J.Y. Yang, M.A. Della-Fera and C.A. Baile, Guggulsterone inhibits adipocyte differentiation and induces apoptosis in 3T3-L1 cells, Obesity (Silver Spring) 16 (2008), 16-22.
32. J.Y. Yang, M.A. Della-Fera, C. Nelson-Dooley and C.A. Baile, Molecular mechanisms of apoptosis induced by ajoene in 3T3-L1 adipocytes, Obesity (Silver Spring) 14 (2006), 388-397.
33. J.Y. Yang, M.A. Della-Fera, S. Rayalam and C.A. Baile, Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis, Apoptosis 12 (2007), 1953-1963.