Short-hairpin-RNA-mediated silencing of fucosyltransferase 8 in Chinese-hamster ovary cells for the production of antibodies with enhanced antibody immune effector function

Biotechnology and Applied Biochemistry

Volumn 53, Issue 1, 2009, Pages 31-37

  Beuger, Vincent ^a   Künkele, Klaus Peter ^a   Koll, Hans ^a   Gärtner, Achim ^a   Bähner, Monica ^a   Burtscher, Helmut ^a   Klein, Christian ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Antibody; Chinese hamster ovary cells (CHO cells); Electrospray ionization MS; ESI MS; Fucosyltransferase 8 (Fut8); Gene silencing; Immune effector function; Short hairpin RNA (shRNA)

Indexed keywords

CHINESE-HAMSTER OVARY CELLS (CHO CELLS); ELECTROSPRAY-IONIZATION MS; ESI-MS; FUCOSYLTRANSFERASE 8 (FUT8); GENE SILENCING; IMMUNE EFFECTOR FUNCTION; SHORT-HAIRPIN RNA (SHRNA);

CELL MEMBRANES; ELECTROSPRAY IONIZATION; GENETIC ENGINEERING; IONIZATION; MEATS; NUCLEIC ACIDS; RNA;

ANTIBODIES;

AGGLUTININ; ANTIBODY; FUCOSYLTRANSFERASE 8; GLYCOSYLTRANSFERASE; MESSENGER RNA; NERVE GROWTH FACTOR RECEPTOR; SHORT HAIRPIN RNA; SOMATOMEDIN C RECEPTOR ANTIBODY; UNCLASSIFIED DRUG; FUCOSYLTRANSFERASE; GLYCOPROTEIN 6 ALPHA L FUCOSYLTRANSFERASE; GLYCOPROTEIN 6-ALPHA-L-FUCOSYLTRANSFERASE; RECOMBINANT PROTEIN; SMALL INTERFERING RNA; SOMATOMEDIN C RECEPTOR;

ANIMAL CELL; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ANTIBODY PRODUCTION; ARTICLE; CELL CLONE; CHO CELL; CONTROLLED STUDY; FUCOSYLATION; GENE EXPRESSION; GENE SILENCING; NONHUMAN; WILD TYPE; ANIMAL; CRICETULUS; GENETICS; HAMSTER; IMMUNOLOGY; METABOLISM; MOLECULAR CLONING; RNA INTERFERENCE;

CRICETULUS GRISEUS; LENS CULINARIS;

ANIMALS; ANTIBODIES; ANTIBODY FORMATION; ANTIBODY-DEPENDENT CELL CYTOTOXICITY; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; FUCOSYLTRANSFERASES; GENE EXPRESSION; RECEPTOR, IGF TYPE 1; RECOMBINANT PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 65649154135     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20080220     Document Type: Article

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