An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli

Journal of Biomolecular NMR

Volumn 11, Issue 1, 1998, Pages 97-102

  Cai, Mengli ^a   Huang, Ying ^a   Sakaguchi, Kazuyasu ^b   Clore, G Marius ^a   Gronenborn, Angela M ^a   Craigie, Robert ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Heteronuclear NMR; Isotope labeling; Protein expression

Indexed keywords

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1;

CARBON; INTEGRASE; NITROGEN; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; COST BENEFIT ANALYSIS; ESCHERICHIA COLI; GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POINT MUTATION;

CARBON ISOTOPES; COST-BENEFIT ANALYSIS; ESCHERICHIA COLI; HIV INTEGRASE; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; POINT MUTATION; RECOMBINANT PROTEINS;

EID: 0031627465     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008222131470     Document Type: Article

References (8)

1. Bax, A. and Grzesiek, S. (1993) Acc. Chem. Res., 26, 131-138.
2. Cai, M., Zheng, R., Caffrey, M., Craigie, R., Clore, G.M. and Gronenborn, A.M. (1997) Nat. Struct. Biol., 4, 567-577.
3. Clore, G.M. and Gronenborn, A.M. (1991a) Science, 252, 1390-1399.
4. Clore, G.M. and Gronenborn, A.M. (1991b) Prog. Nucl. Magn. Reson. Spectrosc., 23, 43-92.
5. Muchmore, D.C., McIntosh, L.P., Russel, C.B., Anderson, D.E. and Dahlquist, F.W. (1989) Methods Enzymol., 177, 44-73.
6. Reilly, D. and Fairbrother, W.J. (1994) J. Biomol. NMR, 4, 459-462.
7. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorf, J.W. (1990) Methods Enzymol., 185, 60-89.
8. Venters, R.A., Calderone, T.L., Spicer, L.D. and Fierke, C.A. (1991) Biochemistry, 30, 4491-1194.