Sticky PCR: A PCR-based protocol for targeted protein engineering

Biotechnology Journal

Volumn 4, Issue 4, 2009, Pages 544-553

  Yamabhai, Montarop ^a  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

Author keywords

Cloning; Mutagenesis; PCR; Protein engineering

Indexed keywords

CLONING VECTORS; COHESIVE ENDS; DELETION MUTATIONS; DNA FRAGMENTS; DOMAIN SWAPPING; EPIDERMAL GROWTH FACTOR RECEPTORS; MEMBRANE LOCALIZATIONS; NOVEL METHODS; OLIGONUCLEOTIDE PRIMERS; PCR; POLYMERASE CHAIN REACTIONS; PROTEIN ENGINEERING; RESTRICTION ENDONUCLEASE;

AMPLIFICATION; CLONING; DNA; ENGINEERING; GENES; MUTAGENESIS; NUCLEIC ACIDS; OLIGONUCLEOTIDES;

BIOCHEMICAL ENGINEERING;

DNA FRAGMENT; ENDONUCLEASE;

ARTICLE; BIOTECHNOLOGICAL PROCEDURES; BIOTECHNOLOGICAL PRODUCTION; DNA VECTOR; GENE AMPLIFICATION; GENE CONSTRUCT; MOLECULAR CLONING; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING;

BASE SEQUENCE; CLONING, MOLECULAR; DNA; DNA PRIMERS; DNA RESTRICTION ENZYMES; DNA, RECOMBINANT; GENE DELETION; GENETIC VECTORS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY;

EID: 65549144980     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800198     Document Type: Article

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