Sticky-end PCR: New method for subcloning

BioTechniques

Volumn 25, Issue 2, 1998, Pages 206-208

  Zeng, Gang ^a,b  

^a GEORGETOWN UNIVERSITY   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; LIGASE; OLIGODEOXYRIBONUCLEOTIDE; PLASMID DNA; PLASMID VECTOR;

ARTICLE; GENE THERAPY; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION SITE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0031828101     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98252bm05     Document Type: Article

References (4)

1. Delidow, B.C. 1993. Molecular cloning of polymerase chain reaction fragments with cohesive ends, p. 217-228. In B.A.White (Ed.), Methods in Molecular Biology: PCR Protocols-Current Methods and Applications. Vol. 15. Humana Press. Totowa.
2. Mole, S.E., R.D. Iggo and D.P. Lane. 1989. Using the polymerase chain reaction to modify expression plasmids for epitope mapping. Nucleic Acids Res. 17:3319.
3. Shuldiner, A.R., K. Tanner, L.A. Scott, C.A. Moore and J. Roth. 1991. Ligase-free subcloning: a versatile method to subclone polymerase chain reaction (PCR) products in a single day. Anal. Biochem. 194:9-15.
4. Temesgen, B. and K. Eschrich. 1996. Simplified method for ligase-free cloning of PCR products. BioTechniques 21:828-832.