Chapter 16 Monitoring Mitochondrial Dynamics with Photoactivateable Green Fluorescent Protein

Methods in Enzymology

Volumn 457, Issue B, 2009, Pages 289-304

  Molina, Anthony J A ^a   Shirihai, Orian S ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOTOACTIVATEABLE GREEN FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; FLUORESCENT DYE;

ASSAY; DISSOCIATION; DYNAMICS; GENE TARGETING; MEMBRANE FUSION; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; NONHUMAN; PARAMETER; PRIORITY JOURNAL; REVIEW; ADENOVIRUS; ANIMAL; ARTICLE; CELL LINE; CELL MEMBRANE POTENTIAL; CONFOCAL MICROSCOPY; GENE VECTOR; GENETICS; HUMAN; LENTIVIRINAE; METABOLISM; METHODOLOGY; PHOTOCHEMISTRY; ULTRASTRUCTURE;

ADENOVIRIDAE; ANIMALS; CELL LINE; FLUORESCENT DYES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; MEMBRANE POTENTIALS; MICROSCOPY, CONFOCAL; MITOCHONDRIA; PHOTOCHEMISTRY;

EID: 65449184157     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05016-2     Document Type: Review

References (22)

1. Bereiter-Hahn J., and Voth M. Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria. Microsc. Res. Tech. 27 (1994) 198-219
2. Chan D.C. Mitochondria: Dynamic organelles in disease, aging, and development. Cell 125 (2006) 1241-1252
3. Chen H., Detmer S.A., Ewald A.J., Griffin E.E., Fraser S.E., and Chan D.C. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160 (2003) 189-200
4. Detmer S.A., and Chan D.C. Functions and dysfunctions of mitochondrial dynamics. Nat. Rev. Mol. Cell Biol. 8 (2007) 870-879
5. Griffin E.E., Detmer S.A., and Chan D.C. Molecular mechanism of mitochondrial membrane fusion. Biochim. Biophys. Acta 1763 (2006) 482-489
6. Herzig S., and Martinou J.C. Mitochondrial dynamics: To be in good shape to survive. Curr. Mol. Med. 8 (2008) 131-137
7. Hoffmann H.P., and Avers C.J. Mitochondrion of yeast: Ultrastructural evidence for one giant, branched organelle per cell. Science 181 (1973) 749-751
8. Jeong S.Y., and Seol D.W. The role of mitochondria in apoptosis. BMB Rep. 41 (2008) 11-22
9. Karbowski M., Arnoult D., Chen H., Chan D.C., Smith C.L., and Youle R.J. Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis. J. Cell Biol. 164 (2004) 493-499
10. Legros F., Lombes A., Frachon P., and Rojo M. Mitochondrial fusion in human cells is efficient, requires the inner membrane potential, and is mediated by mitofusins. Mol. Biol. Cell 13 (2002) 4343-4354
11. Malka F., Guillery O., Cifuentes-Diaz C., Guillou E., Belenguer P., Lombes A., and Rojo M. Separate fusion of outer and inner mitochondrial membranes. EMBO Rep. 6 (2005) 853-859
12. Mattenberger Y., James D.I., and Martinou J.C. Fusion of mitochondria in mammalian cells is dependent on the mitochondrial inner membrane potential and independent of microtubules or actin. FEBS Lett. 538 (2003) 53-59
13. Meeusen S., McCaffery J.M., and Nunnari J. Mitochondrial fusion intermediates revealed in vitro. Science 305 (2004) 1747-1752
14. Mozdy A.D., and Shaw J.M. A fuzzy mitochondrial fusion apparatus comes into focus. Nat. Rev. Mol. Cell Biol. 4 (2003) 468-478
15. Nunnari J., Marshall W.F., Straight A., Murray A., Sedat J.W., and Walter P. Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Mol. Biol. Cell 8 (1997) 1233-1242
16. Partikian A., Olveczky B., Swaminathan R., Li Y., and Verkman A.S. Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J. Cell Biol. 140 (1998) 821-829
17. Patterson G.H., and Lippincott-Schwartz J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297 (2002) 1873-1877
18. Scott S.V., Cassidy-Stone A., Meeusen S.L., and Nunnari J. Staying in aerobic shape: How the structural integrity of mitochondria and mitochondrial DNA is maintained. Curr. Opin. Cell Biol. 15 (2003) 482-488
19. 2+ signaling. Biochim. Biophys. Acta 1763 (2006) 442-449
20. Twig G., Elorza A., Molina A.J., Mohamed H., Wikstrom J.D., Walzer G., Stiles L., Haigh S.E., Katz S., Las G., Alroy J., Wu M., et al. Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J. 27 (2008) 433-446
21. Twig G., Graf S.A., Wikstrom J.D., Mohamed H., Haigh S.E., Elorza A., Deutsch M., Zurgil N., Reynolds N., and Shirihai O.S. Tagging and tracking individual networks within a complex mitochondrial web with photoactivatable GFP. Am. J. Physiol. Cell Physiol. 291 (2006) C176-C184
22. Zuchner S., Mersiyanova I.V., Muglia M., Bissar-Tadmouri N., Rochelle J., Dadali E.L., Zappia M., Nelis E., Patitucci A., Senderek J., Parman Y., Evgrafov O., et al. Mutations in the mitochondrial GTPase mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A. Nat. Genet. 36 (2004) 449-451