Tagging and tracking individual networks within a complex mitochondrial web with photoactivatable GFP

American Journal of Physiology - Cell Physiology

Volumn 291, Issue 1, 2006, Pages

  Twig, Gilad ^a   Graf, Solomon A ^a   Wikstrom, Jakob D ^a   Mohamed, Hibo ^a   Haigh, Sarah E ^a   Elorza, Alvaro ^a   Deutsch, Motti ^b   Zurgil, Naomi ^b   Reynolds, Nicole ^a   Shirihai, Orian S ^a,c  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b BAR ILAN UNIVERSITY   (Israel)

^c TUFTS UNIVERSITY   (United States)

Author keywords

Fission; Fusion; Green fluorescent protein; Heterogeneity; Membrane potential; Tetramethylrhodamine ethyl ester perchlorate

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE POTENTIAL; CONFOCAL MICROSCOPY; FIBROBLAST; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MITOCHONDRIAL MEMBRANE; NONHUMAN; PHOTOACTIVATION; PRIORITY JOURNAL;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; GREEN FLUORESCENT PROTEINS; HUMANS; K562 CELLS; MEMBRANE POTENTIALS; MICROSCOPY, CONFOCAL; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; STAINING AND LABELING;

EID: 33745714433     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00348.2005     Document Type: Article

References (32)

1. Amchenkova AA, Bakeeva LE, Chentsov YS, Skulachev VP, and Zorov DB. Coupling membranes as energy-transmitting cables. I. Filamentous mitochondria in fibroblasts and mitochondrial clusters in cardiomyocytes. J Cell Biol 107: 481-495, 1988.
2. Aon MA, Cortassa S, and O'Rourke B. Percolation and criticality in a mitochondrial network. Proc Natl Acad Sci USA 101: 4447-4452, 2004.
3. Bakeeva LE, Chentsov Y, and Skulachev VP. Mitochondrial framework (reticulum mitochondriale) in rat diaphragm muscle. Biochim Biophys Acta 501: 349-369, 1978.
4. Bereiter-Hahn J. Behavior of mitochondria in the living cell. Int Rev Cytol 122: 1-63, 1990.
5. Chen H, Chomyn A, and Chan DC. Disruption of fusion results in mitochondrial heterogeneity and dysfunction. J Biol Chem 280: 26185-26192, 2005.
6. Collins TJ, Berridge MJ, Lipp P, and Bootman MD. Mitochondria are morphologically and functionally heterogeneous within cells. EMBO J 21: 1616-1627, 2002.
7. Collins TJ and Bootman MD. Mitochondria are morphologically heterogeneous within cells. J Exp Biol 206: 1993-2000, 2003.
8. De Giorgi F, Lartigue L, and Ichas F. Electrical coupling and plasticity of the mitochondrial network. Cell Calcium 28: 365-370, 2000.
9. Diaz G, Falchi AM, Gremo F, Isola R, and Diana A. Homogeneous longitudinal profiles and synchronous fluctuations of mitochondrial transmembrane potential. FEBS Lett 475: 218-224, 2000.
10. Frey TG and Mannella CA. The internal structure of mitochondria. Trends Biochem Sci 25: 319-324, 2000.
11. Frey TG, Renken CW, and Perkins GA. Insight into mitochondrial structure and function from electron tomography. Biochim Biophys Acta 1555: 196-203, 2002.
12. Graf SA, Haigh SE, Corson ED, and Shirihai OS. Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). J Biol Chem 279: 42954-42963, 2004.
13. Jakobs S, Martini N, Schauss AC, Egner A, Westermann B, and Hell SW. Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p. J Cell Sci 116: 2005-2014, 2003.
14. Karbowski M, Arnoult D, Chen H, Chan DC, Smith CL, and Youle RJ. Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis. J Cell Biol 164: 493-499, 2004.
15. Kroemer G and Reed JC. Mitochondrial control of cell death. Nat Med 6: 513-519, 2000.
16. Lee YJ, Jeong SY, Karbowski M, Smith CL, and Youle RJ. Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis. Mol Biol Cell 15: 5001-5011, 2004.
17. Malka F, Guillery O, Cifuentes-Diaz C, Guillou E, Belenguer P, Lombes A, and Rojo M. Separate fusion of outer and inner mitochondrial membranes. EMBO Rep 6: 853-859, 2005.
18. Meeusen S, McCaffery JM, and Nunnari J. Mitochondrial fusion intermediates revealed in vitro. Science 305: 1747-1752, 2004.
19. Nicholls DG and Budd SL. Mitochondria and neuronal survival. Physiol Rev 80: 315-360, 2000.
20. Nicholls DG and Ward MW. Mitochondrial membrane potential and neuronal glutamate excitotoxicity: mortality and millivolts. Trends Neurosci 23: 166-174, 2000.
21. O'Reilly CM, Fogarty KE, Drummond RM, Tuft RA, and Walsh JV Jr. Quantitative analysis of spontaneous mitochondrial depolarizations. Biophys J 85: 3350-3357, 2003.
22. Partikian A, Olveczky B, Swaminathan R, Li Y, and Verkman AS. Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J Cell Biol 140: 821-829, 1998.
23. Pozzan T and Rizzuto R. High tide of calcium in mitochondria. Nat Cell Biol 2: E25-E27, 2000.
24. Scaduto RC Jr and Grotyohann LW. Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J 76: 469-477, 1999.
25. Skulachev VP. Mitochondrial filaments and clusters as intracellular power-transmitting cables. Trends Biochem Sci 26: 23-29, 2001.
26. Smiley ST, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith TW, Steele GD Jr, and Chen LB. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc Natl Acad Sci USA 88: 3671-3675, 1991.
27. Sugioka R, Shimizu S, and Tsujimoto Y. Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis. J Biol Chem 279: 52726-52734, 2004.
28. Sunray M, Zurgil N, Shafran Y, and Deutsch M. Determination of individual cell Michaelis-Menten constants. Cytometry 47: 8-16, 2002.
29. 2+-mediated apoptosis. Mol Cell 16: 59-68, 2004.
30. Tandler B and Hoppel CL. Possible division of cardiac mitochondria. Anat Rec 173: 309-323, 1972.
31. Westermann B. Merging mitochondria matters: cellular role and molecular machinery of mitochondrial fusion. EMBO Rep 3: 527-531, 2002.
32. Zurgil N, Solodeev I, Gilburd B, Shafran Y, Afrimzon E, Avtalion R, Shoenfeld Y, and Deutsch M. Monitoring the apoptotic process induced by oxidized low-density lipoprotein in jurkat T-lymphoblast and U937 monocytic human cell lines. Cell Biochem Biophys 40: 97-113, 2004.