Engineering an ultra-stable affinity reagent based on top 7

Protein Engineering, Design and Selection

Volumn 22, Issue 5, 2009, Pages 325-332

  Boschek, Curt B ^a   Apiyo, David O ^a,b   Soares, Thereza A ^a   Engelmann, Heather E ^a   Pefaur, Noah B ^a,c   Straatsma, Tjerk P ^a   Baird, Cheryl L ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b Immunoassay Business Center   (United States)

^c Department of Protein Biochemistry ^*  (United States)

Author keywords

Engineering affinity; Molecular dynamics simulations; Protein scaffold; Protein stability; Top7

Indexed keywords

AFFINITY REAGENTS; CHEMICAL DENATURATIONS; CONFORMATIONAL STABILITIES; MOLECULAR DYNAMICS SIMULATIONS; PEPTIDE SEQUENCES; PROTEIN STABILITY; SECONDARY STRUCTURES; SPECIFIC RECOGNITION; SPECIFIC SITES; STRUCTURAL COMPLEXITY; STRUCTURAL EFFECTS; THERAPEUTIC APPLICATIONS; TOP7;

AMINES; APPLICATIONS; CHEMICAL STABILITY; DYNAMICS; MOLECULAR DYNAMICS; SCAFFOLDS; STABILITY;

ANTIBODIES;

CD4 ANTIBODY; GUANIDINE; PROTEIN TOP7; SCAFFOLD PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN SYNTHESIS;

AFFINITY LABELS; AMINO ACID SEQUENCE; ANTIGENS, CD4; CARRIER PROTEINS; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; COMPUTATIONAL BIOLOGY; COMPUTER SIMULATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN ENGINEERING; SENSITIVITY AND SPECIFICITY;

EID: 65349192961     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp007     Document Type: Article

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