The och1 mutant of schizosaccharomyces pombe produces galactosylated core structures of n-linked oligosaccharides

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 2, 2009, Pages 407-414

  Ohashi, Takao ^a,c,d   Ikeda, Yuka ^a   Tanaka, Naotaka ^a   Nakakita, Shin Ichi ^a   Natsuka, Shunji ^b   Giga Hama, Yuko ^c   Takegawa, Kaoru ^a,d  

^a KAGAWA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c ASAHI GLASS CO LTD   (Japan)

^d KYUSHU UNIVERSITY   (Japan)

Author keywords

1,6 mannosyltransferase; Galactosylation; Glycosylation; Och1; Schizosaccharomyces pombe

Indexed keywords

BLOT ANALYSIS; CELL SURFACES; CHAIN ELONGATIONS; CORE STRUCTURES; FISSION YEASTS; GALACTOSE RESIDUES; GALACTOSYLATION; GENE PRODUCTS; IN-VITRO; KEY ENZYMES; N-LINKED; OCH1; SCHIZOSACCHAROMYCES POMBE; STRUCTURAL HETEROGENEITIES; SUBSTRATE SPECIFICITIES; YEAST SACCHAROMYCES CEREVISIAE;

CELL MEMBRANES; ESTERIFICATION; GLYCOSYLATION; OLIGOSACCHARIDES; YEAST;

STRUCTURAL ANALYSIS;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

GALACTOSE; HYDRAZINE; HYDRAZINE DERIVATIVE; MANNOSYLTRANSFERASE; MUTANT PROTEIN; OCH1 PROTEIN, S POMBE; OLIGOSACCHARIDE; PYRIDINE; PYRIDINE DERIVATIVE; SCHIZOSACCHAROMYCES POMBE PROTEIN;

AMINATION; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CYTOLOGY; ENDOPLASMIC RETICULUM; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MUTATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SCHIZOSACCHAROMYCES;

AMINATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENDOPLASMIC RETICULUM; GALACTOSE; HYDRAZINES; MAGNETIC RESONANCE SPECTROSCOPY; MANNOSYLTRANSFERASES; MUTANT PROTEINS; MUTATION; OLIGOSACCHARIDES; PYRIDINES; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

EID: 65249107215     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.80712     Document Type: Article

References (44)

1. Bush, D. A., Horeisberger, M., Horman, I., and Wursch, P., The wall structure of Schizosacchatomyces pombe. J. Gen. Mico-biol, 81, 199-206 (1974).
2. Ballou, C. E., Ballou, L., and Ball, G., Schizosaccharomyces pombe glycosylation mutant with altered cell surface properties. Proc. Natl. Acad. Sci USA, 91, 9327-9331 (1994).
3. Gemmill, T. R., and Trimble, R. B., Schizosaccharomyces pombe produces novel pyruvate-containing JV-linked oligosaccharides. J. Biol. Chem., 271, 25945-25949 (1996).
4. Gemmill, T. R., and Trimble, R. B., All pyruvated galactose in Schizosaccharomyces pombe JV-glycans is present in the terminal disaccharide, 4,6-[(R)-(l-carboxyehylidine)]-Galβ1,3Galαl-. Glycobiology, 8, 1087-1095 (1998).
5. Andreishcheva, E. N., Kunkel, J. P., Gemmill, T. R., and Trimble, R. B., Five genes involved in biosyntehsis of the pyruvylated Galβ1,3-epitope in Schizosaccharomyces pombe JV-linked glycans. J. Biol. Chem., 279, 35644-35655 (2004).
6. Ballou, C. E., Isolation, characterization and properties of Saccharomyces cerevisiae mnn mutants with non-conditional protein glycosylation defects. Methods Enzymol., 185, 440-470 (1990).
7. Lussier, M., Sdica, A. M., Bussereau, M., Jacquet, M., and Bussey, H., The Ktrlp, Ktr3p, and Kre2p/Mntlp mannosyl-transferases paticipate in the elaboration of yeast O- and JV-linked carbohydrate chains. J. Biol. Chem., 272, 15527-15531 (1997).
8. Lussier, M., Sdicu, A. M., Winnett, E., Vo, D. H., Sheraton, J., Dusterhoft, A., Sorms, R. K., and Bussey, H., Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism. Yeast, 13, 267-274 (1997).
9. Ziegler, F. D., Gemmil, T. R., and Trimble, R. B., Glycoprotein synthesis in yeast. J. Biol. Chem., 269, 12527-12535 (1994).
10. Ballou, L., and Ballou, C. E., Schizosaccharomyces pombe mutants are defective in glycoprotein galactosylation. Proc. Natl. Acad. Sci USA, 92, 2790-2794 (1995).
11. 9GlcNAc isomers: role of the Golgi GMA12 galactosyltransferase in core glycans galactosylation. Glycobioloby, 9, 497-505 (1999).
12. Nakayama, K., Nagasu, T., Shinma, Y., Kuromitsu, J., and Jigami, Y., OCH1 encodes a novel membrane bound manno-syltransferase: outer chain elongation of asparagines-linked oligosaccharides. EMBO J., 11, 2511-2519 (1992).
13. + encodes a-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides. FEBS Lett., 489, 75-80 (2001).
14. Kitajima, T., Chiba, Y., and Jigami, Y., Saccharomyces cerevisiae a 1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule. FEBS J., 273, 5074-5085 (2006).
15. Nakayama, K., Nakanishi-Shindo, Y., Tanaka, A., Haga-Toda, Y., and Jigami, Y., Substrate specificity of a-1,6-mannosyltransferase that initiates JV-linked mannose outer chain elongation in Saccharomyces cerevisiae. FEBS Lett., 412, 547-550 (1997).
16. Murakami, K., and Sugio, N., Japan Patent JP 1997000261-A1 (Jan. 7, 1997).
17. Stephanie, B.-V., Boisramé, A., and Beckerich, J.-M., Identification and characterization of two a-l,6-mannosyltransferases, Anllp and Ochlp, in the yeast Yarrowia lipolytica. Microbiology, 150, 2185-2195 (2004).
18. Kuroda, K., Kobayashi, K., Tsumura, H., Komeda, T., Chiba, Y., and Jigami, Y., Production of Man5GlcNAc2-type sugar chain by the methylotrophic yeast Ogataea minuta. FEMS Yeast Res., 6, 1052-1062 (2006).
19. Kim, M. K., Kim, E. J., Kim, J.-Y., Park, J.-S., Oh, D.-B., Shimma, Y.-L, Chiba, Y., Jigami, Y., Rhee, S. K., and Kang, H. A., Functional characterization of the Hansenula polymorpha HOC1, OCH1, and OCR1 genes as members of the yeast OCH1 mannosyltransferase family involved in protein glycosylation. J. Biol. Chem., 281, 6261-6271 (2006).
20. Bates, S., Hughes, H. B., Munro, C. A., Thomas, W. P. H., MacCallum, D. M., Bertram, G., Atrih, A., Ferguson, M. A. J., Brown, A. J. P., Odds, F. C, and Gow, N. A. R., Outer chain JV-glycans are required for cell wall integrity and virulence of Candida albicans. J. Biol. Chem., 281, 90-98 (2006).
21. Nakanishi-Shindo, Y., Nakayama, K., Tanaka, A., Toda, Y., and Jigami, Y., Structure of the JV-linked oligosaccharides that show the complete loss of a-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1 alg3 mutants of Saccharomyces cerevisiae. J. Biol. Chem., 268, 26338-26345 (1993).
22. Vervecken, W., Kaigorodov, V., Callewaert, N., Geysens, S., Vusser, K. D., and Contreras, R., In vivo synthesis of mammalian-like, hybrid-type JV-glycans in Pichia pastoris. Appl. Environ. Microbiol., 70, 2639-2646 (2004).
23. Song, Y., Choi, M. H., Park, J.-N., Kim, M. W., Kim, E. J., Kang, H. A., and Kim, J.-Y., Engineering of the yeast Yarrowia lipolytica for the production of glycoproeins lacking the outer-chain mannose residues of JV-glycans. Appl. Environ. Microbiol., 73, 4446-4454 (2007).
24. Giga-Hama, Y., Tohda, H., Takegawa, K., and Kumagai, H., Schizosaccharomyces pombe minimum genome factory. Bio-technol Appl. Biochem., 46, 147-155 (2007).
25. Alfa, C, Fantes, P., Hyams, J., Mcleod, M., and Warbrick, E., "Experiments with Fission Yeast: A Laboratory Manual," Cold Spring Harbor Laboratory Press, New York (2003).
26. Takegawa, K., Tanaka, N., Tabuchi, M., and Iwahara, S., Isolation and characterization of a glycosylation mutant from Schizosaccharomyces pombe. Biosci. Biotechnol Biochem., 60, 1156-1159 (1996).
27. + gene encodes an UDP-galactose transpoter homologue required for protein galactosylation. Biochem. Biophys. Res. Commun., 232, 121-125 (1997).
28. Tanaka, N., and Takegawa, K., Functional characterization of Gmslp/UDP-galactose transporter in Schizosaccharomyces pombe. Yeast, 18, 745-757 (2001).
29. Tanaka, N., Konomi, M., Osumi, M., and Takegawa, K., Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity. Yeast, 18, 903-914 (2001).
30. Okazaki, K., Okazaki, N., Kume, K., Jinno, S., Tanaka, K., and Okayama, H., High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res., 18, 6485-6489 (1990).
31. Morita, T., and Takegawa, K., A simple and efficient procedure for transformation of Schizosaccharomyces pombe. Yeast, 21, 613-617 (2004).
32. Peat, S., Whelan, W. J., and Edwards, T. E., Polysaccharides of baker's yeast. Part IV. Mannan. J. Chem. Soc, 29-34 (1961).
33. Hase, S., Ikenaka, T., and Matsushima, Y., Structure analysis of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound. Biochem. Biophys. Res. Commun., 85, 257-263 (1978).
34. Hase, S., High-performance liquid chromatography of pyridy-laminated saccharides. Methods Enzymol, 230, 225-237 (1994).
35. 1-3 O-linked oligosaccharides. Glycobiology, 9, 507-515 (1999).
36. + gene products from Schizosaccharomyces pombe. Eur. J. Biochem., 257, 630-637 (1998).
37. Ikeda, Y., Ohashi, T., Tanaka, N., and Takegawa, K., Identification and characterization of a gene required for α1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe. FEMS Yeast Res., 9, 115-125 (2009).
38. Cohen, R., and Ballou, C. E., Linkage and sequence analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopy. Biochemistry, 19, 4353-4358 (1980).
39. Movsichoff, F., Castro, O. A., and Parodi, A., Characterization of Schizosaccharomyces pombe ER α-mannosidase: a reevalua-tion of the role of the enzyme on ER-associated degradation. Mol. Biol. Cell, 16, 4714-4724 (2005).
40. Li, Y.-T., and Li, S. C, α-Galactosidase from figs. Methods Enzymol., 28, 714-720 (1972).
41. Choi, B.-K., Bobrowicz, P., Davidson, R. C, Hamilton, S. R., Kung, D. H., Li, H., Miele, R. G., Nett, J. H., Wildt, S., and Gerngross, T. U., Use of combinatorial genetic libraries to humanize JV-linked glycosylation in the yeast Pichia pastoris. Proc. Natl. Acad. Sci. USA, 100, 5022-5027 (2003).
42. Hamilton, S. R., Bobrowicz, P., Bobrowicz, B., Davidson, R. C, Li, H., Mitchell, T., Nett, J. H., Rausch, S., Stadheim, T. A., Wischnewski, H., Wildt, S., and Gerngross, T. U., Production of complex human glycoprotein in yeast. Science, 301, 1244-1246 (2003).
43. Hamilton, S. R., Davidson, R. C, Sethuraman, N., Nett, J. H., Jiang, Y., Rios, S., Bobrowicz, P., Stadheim, T. A., Li, H., Choi, B.-K., Hopkins, D., Wischnewski, H., Roser, J., Mitchell, T., Strawbridge, R. R., Hooped, J., Wildt, S., and Gerngross, T. U., Humanization of yeast to produce complex terminally sialylated glycoproeins. Science, 313, 1441-1443 (2006).
44. Oh, D.-B., Park, J.-S., Kim, M. W., Cheon, S. A., Kim, E. J., Moon, H. Y., Kwon, O., Rhee, S. K., and Kang, H. A., Glycoengineering of the methylotrophic yeast Hansenula poly-morpha for the production of glycoproteins with trimannosyl core JV-glycan by blocking core oligosaccharide assembly. Biotechnol. J., 3, 659-668 (2008).